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AB270552

SimpleStep ELISA® Custom ELISA Kit

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(1 Publication)

SimpleStep ELISA® Custom ELISA Kit allows you to develop your own SimpleStep ELISA® using any antibody pair.

- No plate coating needed
- Possibility to measure different target on the same plate
- All components included, just add the antibody pair
- Streamline your ELISA® to a single-wash format if desired
4 Images
ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
  • ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
  • ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

Schematic representation of how the kit works

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
  • ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

SimpleStep ELISA® Custom ELISA Kit and conventional passive coating approach were compared. With ab270552, 10x less capture antibody can be used without affecting the assay performance.

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
  • ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

Other solution-phase binding ELISA formats typically use biotin to immobilize immunocomplexes. These can constrain the sample types that can be used with the assay. In contrast, ab270552 has been specifically designed to be used with most sample matrices without interference.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

SimpleStep ELISA® Custom ELISA Kit (ab270552) is the latest cutting-edge technology with a built-in system for antibody labeling. Built on Abcam’s patented CaptSureTM technology this system enables the rapid and easy development of in-house ELISAs using any antibody pair, while eliminating the need to perform plate coating procedures.

It is powered by the combination of proprietary technologies, enabling you to reduce the amount of capture antibody required, measure different targets on the same plate with different assay antibody pairs, and streamline your ELISA to a single-wash format if desired.

The capture antibody of interest is quickly and easily conjugated with the CaptSureTM Peptide supplied within the kit. The interaction between the antibody coated on the plate and the CaptSureTM Peptide immobilizes the immune-complex.

The detection antibody is similarly conjugated with HRP using the Lightning-Link® HRP conjugation reagents, also supplied in the kit. Thus, with minimal hands-on time, you can set up both of your antibodies to ensure the best performance of your ELISA.

This kit has ready-to-go assay plates, pre-coated with antibody, which are specific for the CaptSureTM Peptide.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 21:114 PubMed33596916

2021

Targeting SNHG3/miR-186-5p reverses the increased m6A level caused by platinum treatment through regulating METTL3 in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxin Zhang,Minghua Bai,Li Wang,Ning Lu,Jia Wang,Rong Yan,Manli Cui,Honglin Yan,Lingmin Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com