SimpleStep ELISA® Peptide Check Kit

SimpleStep ELISA^® Peptide Check Kit (ab270053) is a quick and simple immunochromatography test that allows you to confirm the successful conjugation of Capture Peptide to your desired antibody without any specialized or costly equipment. This kit has been developed exclusively for the SimpleStep ELISA® Custom ELISA Kit (ab270552) and SimpleStep ELISA® Capture Peptide Conjugation Kit (ab270690).

The key component of the kit is a nitrocellulose membrane containing a 'Test line' of Capture Antibody and 40 nm Gold-Capture Antibody conjugate for detection. When the Capture Peptide tagged antibody is mixed with the 40 nm Gold-Capture Antibody conjugate, it forms a complex that flows along the nitrocellulose membrane and binds to the Capture Antibody on the Test line. Two visible red lines on the strip confirm successful conjugation with the SimpleStep ELISA® Capture Peptide Conjugation Kit.

Benefits:

Quick and easy qualitative test to check success of conjugation with SimpleStep ELISA® Capture Peptide Conjugation Kit (ab270690)

Sensitive – range of detection is between 0.1 μg/ml and 1 μg/ml

Requires small volumes of conjugate (30 μl)

No costly equipment required

General notes:

This product is manufactured by Expedeon, an Abcam company. It was previously called CaptSure™ Peptide Check&Go!. Expedeon product code 4010-0030 is the same as the 30-test size of this product.