AB186036

SMAD1 (pS463/465) ELISA Kit

SimpleStep
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(1 Publication)

SMAD1 (pS463/465) ELISA Kit is a single-wash 90-min Simplestep used to quantify SMAD1 (pS463/S465) with a sensitivity of 10 µg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Semi-quantitative sandwich ELISA - 450 nm readout : works on any standard plate reader

6 Images

ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

ELISA

Supplier Data

ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

sELISA

Supplier Data

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of SMAD1 (pS463/S465) are normalized and plotted.

sELISA

Supplier Data

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

Comparison of total SMAD1 expression in different cell lines.

Cell line analysis for Total SMAD1 from 300 μg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).

sELISA

Supplier Data

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

SMAD1 (pS463/S465) phosphorylation in response to dorsomorphin treatment.

Inhibition of SMAD1 (pS463/S465) phosphorylation in HeLa cells in response to dorsomorphin treatment. HeLa cells were cultured in 96-well tissue culture plates and treated with a dose-range of dorsomorphin (30 min). Cells were then stimulated with BMP-4 (30 min) and lysed. Data from quadruplicate measurements of SMAD1 (pS463/S465) and SMAD1 (Total) are plotted.

sELISA

Supplier Data

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

Typical cell lysate dilution series.

Example of a typical SMAD1 (pS463/S465) cell lysate dilution series. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

SMAD1 (pS463/S465) phosphorylation in response to BMP-4 treatment.

Induction of SMAD1 (pS463/S465) phosphorylation in HeLa cells in response to BMP-4 treatment. HeLa cells were cultured in 96-well tissue culture plates, serum-starved and treated (30 min) with a dose-range of BMP-4 before cell lysis. Data from quadruplicate measurements of SMAD1 (pS463/S465) are plotted.

Key facts

Detection method

Colorimetric

Sample types

Tissue Homogenate, Cell Lysate

Reacts with

Mouse, Human

Results type

Semi-Quantitative

Sensitivity

= 10 µg/mL

Range

10 - 500 µg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

SMAD1 (pS463/465) ELISA Kit ab186036 is a rapid single-wash 90-min sandwich ELISA kit to measure SMAD1 (pS463/465) in Cell Lysates and Tissue Homogenates. This Simplestep sensitivity is 10 µg/mL.

How the assay works

SMAD1 (pS463/465) SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

SMAD1 (pS463/465) ELISA Kit ab186036 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

How other researchers are using SMAD1 (pS463/465) ELISA Kit ab186036

SMAD1 (pS463/465) ELISA Kit ab186036 has been used to measure BMP signaling in heterotopic ossification⁽¹⁾.

References:

⁽¹⁾ L. Li, et.al., 2019, PMID: 31508317

Related and Recommended products

SMAD1 (pS463/465) ELISA Kit ab186036 is used to measure SMAD1 phosphorylation in the BMP signaling pathway.

Other ELISA kits to measure the BMP signaling pathway include:

- SMAD3 (pS423/S425) ELISA Kit ab186038
- Human SMAD4 ELISA Kit ab253211
- Human BMP-4 ELISA Kit ab231930

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "C2C12", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "C2C12", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad1 also known as Mothers against decapentaplegic homolog 1 (MADH1) is a protein encoded by the SMAD1 gene in humans. It has a molecular mass of approximately 53 kDa and functions as a receptor-regulated Smad protein. Smad1 primarily transmits signals from the bone morphogenetic proteins (BMPs) a group of growth factors and cytokines. Expression of Smad1 occurs in various tissues including the lung heart and kidney. It plays an important role in mediating signaling pathways within different cellular environments.

Biological function summary

Smad1 participates in the transmission of BMP signals from the cell surface to the nucleus ensuring transcriptional regulation of target genes. In the presence of BMPs Smad1 forms complexes with Smad4 upon activation. These Smad complexes then translocate to the nucleus where they regulate gene expression. Smad1 influences cellular responses such as proliferation differentiation and apoptosis. In particular it plays a significant role in bone development and osteogenesis.

Pathways

Smad1 plays an important role within the BMP signaling pathway which is important for early development and tissue homeostasis. Within this pathway BMPs trigger the phosphorylation of Smad1 which then associates with Smad4 to propagate downstream signaling. Another associated pathway includes the TGF-beta signaling pathway where Smads like Smad2 and Smad3 show functional similarities and differences with Smad1. The interactions of Smad1 with related proteins like Smad4 highlight its significant contribution to cellular processes regulated by these pathways.

Smad1 associates with various pathological conditions. For instance aberrant Smad1 signaling has implications in cancer particularly in processes like tumor progression and metastasis. Disruptions in the BMP pathway involving Smad1 and Smad4 can influence the onset and development of disorders such as pulmonary hypertension where inappropriate cell growth and remodeling occur. Understanding Smad1's role in these contexts provides insights into potential therapeutic avenues for treatment and management of these conditions.

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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic translation 18:142-153 PubMed31508317

2019

Muscle injury promotes heterotopic ossification by stimulating local bone morphogenetic protein-7 production.

Applications

Unspecified application

Species

Unspecified reactive species

La Li,Yangzi Jiang,Hang Lin,He Shen,Jihee Sohn,Peter G Alexander,Rocky S Tuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

SMAD1 (pS463/465) ELISA Kit

ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

Sandwich ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

Key facts

Detection method

Sample types

Reacts with

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise