AB186038

SMAD3 (pS423/S425) ELISA Kit

SimpleStep
What is this?

Be the first to review this product! Submit a review

(5 Publications)

SMAD3 (pS423/S425) ELISA Kit is a single-wash 90-min Simplestep used to quantify SMAD3 (pS423/S425) with a sensitivity of 10 µg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations

View Alternative Names

MADH3, SMAD3, SMAD family member 3, SMAD 3, hSMAD3, JV15-2, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3

7 Images

ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

ELISA

Supplier Data

ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

sELISA

Supplier Data

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Example of a typical SMAD3 (pS423/S425) control lysate dilution series in 1X Cell Extraction Buffer PTR. The SMAD3 lysate dilution series was prepared as described. Raw data values are shown in the table.

sELISA

Supplier Data

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of SMAD3 (pS423/S425) are normalized and plotted.

sELISA

Supplier Data

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Example of a typical SMAD3 (pS423/S425) control lysate dilution series in 1X Cell Extraction Buffer PTR.

The SMAD3 lysate dilution series was prepared as described. Raw data values are shown in the table.

Kit Control lysates are provided at a concentration that give consistent signal between different lots. Lysates are produced and formulated by signal intensity to be consistent to within 30% of the previous lot. As such, Control lysates are not provided with a protein concentration.

sELISA

Supplier Data

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

SMAD3 (pS423/S425) phosphorylation in response to TGF-beta treatment.

Induction of SMAD3 (pS423/S425) phosphorylation in HeLa cells in response to TGF-β treatment. HeLa cells were cultured in 96-well tissue culture plates, serum-starved and treated (60 min) with a dose-range of TGF-β before cell lysis. Data from quadruplicate measurements of SMAD3 (pS423/S425) are plotted.

sELISA

Supplier Data

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

SMAD3 (pS423/S425) control lysate dilution series in 1X Cell Extraction Buffer PTR.

Raw data values are shown in the table.

sELISA

Supplier Data

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

SMAD3 (pS423/S425) phosphorylation in response to SB431542 treatment.

Inhibition of SMAD3 (pS423/S425) phosphorylation in HeLa cells in response to SB431542 treatment. HeLa cells were cultured in 96-well tissue culture plates and treated with a dose-range of SB431542 (60 min). Cells were then stimulated with TGF-β (60 min) and lysed. Data from quadruplicate measurements of SMAD3 (pS423/S425) are plotted.

Key facts

Detection method

Colorimetric

Sample types

Tissue Homogenate, Cell Lysate

Reacts with

Mouse, Human

Results type

Semi-Quantitative

Sensitivity

= 10 µg/mL

Range

10 - 500 µg/mL

Assay time

1h 30m

Assay Platform

Microplate

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

SMAD3 (pS423/S425) ELISA Kit ab186038 is a rapid single-wash 90-min Other Immunoassay to measure SMAD3 (pS423/S425) in cell lysate, tissue homogenate. This SimpleStep sensitivity is 10 µg/mL.

How the assay works

SMAD3 (pS423/S425) SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

SMAD3 (pS423/S425) ELISA Kit ab186038 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "A431", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Intra", "sample": "A431 Extract", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab186038-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"SMAD3 (pS423/S425) Capture Antibody", "number":"AB186038-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Wash Buffer PT", "number":"AB186038-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB186038-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB186038-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized SMAD3 (pS423/S425) Control Lysate", "number":"AB186038-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"SMAD3 (pS423/S425) Detector Antibody", "number":"AB186038-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB186038-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution", "number":"AB186038-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB186038-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB186038-CMP10", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.

Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.

style

left-column

Product protocols

style

left-column

Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

See full target information SMAD3 phospho S423

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:157 PubMed35197550

2022

Fortilin interacts with TGF-β1 and prevents TGF-β receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Decha Pinkaew,Erik Martinez-Hackert,Wei Jia,Matthew D King,Fei Miao,Nicole R Enger,Runglawan Silakit,Kota Ramana,Shi-You Chen,Ken Fujise

International journal of molecular sciences 20: PubMed31835434

2019

Betaglycan (TβRIII) is a Key Factor in TGF-β2 Signaling in Prepubertal Rat Sertoli Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pradeep K Kudipudi,Sebastian P Galuska,Raimund Dietze,Georgios Scheiner-Bobis,Kate L Loveland,Lutz Konrad

Human & experimental toxicology 39:451-463 PubMed31797699

2019

Chitinase-like protein YKL-40 regulates human bronchial epithelial cells proliferation, apoptosis, and migration through TGF-β1/Smads pathway.

Applications

Unspecified application

Species

Unspecified reactive species

R Guan,R Lin,R Jin,L Lu,X Liu,S Hu,L Sun

International journal of molecular sciences 20: PubMed31600983

2019

Syndecan-4 Inhibits the Development of Pulmonary Fibrosis by Attenuating TGF-β Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinori Tanino,Xintao Wang,Takefumi Nikaido,Kenichi Misa,Yuki Sato,Ryuichi Togawa,Takaya Kawamata,Masami Kikuchi,Charles W Frevert,Mishie Tanino,Tetsuhito Kojima,Yoko Shibata

Kidney international 95:1373-1388 PubMed30979564

2019

Mechanisms of GLP-1 receptor-independent renoprotective effects of the dipeptidyl peptidase type 4 inhibitor linagliptin in GLP-1 receptor knockout mice with 5/6 nephrectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed A Hasan,Karoline von Websky,Christoph Reichetzeder,Oleg Tsuprykov,Mohamed M S Gaballa,Jingli Guo,Shufei Zeng,Denis Delić,Harald Tammen,Thomas Klein,Burkhard Kleuser,Berthold Hocher

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

SMAD3 (pS423/S425) ELISA Kit

ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Sandwich ELISA - SMAD3 (pS423/S425) ELISA Kit (AB186038)

Key facts

Detection method

Sample types

Reacts with

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise