SMN ELISA kit is a sandwich ELISA used to quantify Survival Motor Neuron protein with a sensitivity of 50 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- wide measuring range: 50 - 3200 pg/mL
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Human
- Range
- 50 - 3200 pg/mL
- Sensitivity
- = 50 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed:18984161, PubMed:9845364). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed:18984161). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:18984161). To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate (PubMed:18984161). Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits (PubMed:17178713, PubMed:21816274, PubMed:22101937). Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP (PubMed:31799625). Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development (PubMed:23063131). Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (PubMed:26700805). May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
Alternative names
SMN, SMNT, SMN2, SMNC, SMN1, Survival motor neuron protein, Component of gems 1, Gemin-1
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SMN ELISA kit is a sandwich ELISA used to quantify Survival Motor Neuron protein with a sensitivity of 50 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- wide measuring range: 50 - 3200 pg/mL
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Human
- Range
- 50 - 3200 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- = 50 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample 122 pg/mL Spike | n 20 | C.V. 3.2 |
Sample 322 pg/mL Spike | n 20 | C.V. 1.1 |
Sample 928 pg/mL Spike | n 20 | C.V. 0.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample 134 pg/mL Spike | n 12 | C.V. 11.4 |
Sample 378 pg/mL Spike | n 12 | C.V. 8.9 |
Sample 983 pg/mL Spike | n 12 | C.V. 7.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Human PBMC Lysate | Average % | Range 79 - 134 % |
Sample type Mouse brain extract | Average % | Range 83 - 112 % |
Sample type Mouse muscle extract | Average % | Range 76 - 146 % |
Sample type Mouse spinal cord extract | Average % | Range 39 - 69 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's SMN ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of SMN in Human and mouse cell and tissue extracts.
An anti-Human SMN antibody is precoated onto 96-well plates, standards or test samples are added to the wells and incubated at room temperature. The wells are washed and a polyclonal detector antibody specific to SMN is added, followed by incubation at room temperature. After further washing, a horseradish peroxidase (HRP) conjugated anti-species antibody is added to each well and incubated at room temperature. After incubation the excess reagents are washed away. TMB substrate is added to each well and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 450 nm. The intensity of the yellow coloration is directly proportional to the amount of SMN captured in the plate.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMN also known as Survival of Motor Neuron protein or Gemin 1 is an important protein involved in the assembly of small nuclear ribonucleoproteins (snRNPs). It has a molecular mass of approximately 38 kDa. SMN proteins are expressed in almost all tissues with highest levels in spinal cord and brain tissues. They play a mechanical role in Gemin complex assisting RNA processing tasks which are essential in RNA metabolism alongside other Gemin proteins.
- Biological function summary
The SMN protein operates within the SMN-Gemin complex a multi-protein assembly important for snRNP biogenesis and pre-mRNA splicing. This complex coordinates the maturation and transport of snRNPs facilitating the processing of precursor mRNAs in the nucleus. SMN and associated Gemin proteins mediate the assembly of Sm proteins onto snRNA ensuring accurate splicing mechanisms necessary for gene expression.
- Pathways
The SMN protein is notably involved in the chaperone-assisted assembly pathway related to snRNP biogenesis. It plays a central role in the spliceosome assembly process working closely with Sm proteins and other RNA-binding proteins. Importantly SMN interacts with the ribonucleoprotein complex influencing mRNA splicing which integrates into the broader cell signaling pathways such as the cholinergic agonist pathways and RNA splicing pathways that regulate neuromuscular communication.
- Associated diseases and disorders
The deficiency or malfunction of SMN protein directly relates to Spinal Muscular Atrophy (SMA) an autosomal recessive disorder. SMA is characterized by motor neuron degeneration which can be attributed to insufficient SMN protein levels leading to compromised snRNP assembly and splicing. The SMN1 gene deletion or mutation reduces the available SMN protein linking this defect with the severity of SMA. Studies suggest that altering the expression levels of the related SMN2 gene can potentially modulate disease outcomes further emphasizing the connection between SMN protein and SMA pathogenesis.
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2 product images
Sandwich ELISA - SMN ELISA kit (ab136947)
Representative Standard Curve using ab136947.
Sandwich ELISA - SMN ELISA kit (ab136947)
Typical Parallelism Graph.
A parallelism experiment was carried out to determine if the recombinant Human SMN standard accurately determines SMN concentrations in biological matrices. To assess parallelism, values for Human PBMC lysate and mouse tissue extract was obtained from a standard curve using four parameter logistic curve fitting. The observed concentration was plotted against the dilution factor. Parallelism of the curves demonstrates that the antibody binding characteristics are similar enough to allow the accurate determination of analyte levels in diluted samples.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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