STAT1 (pY701) + total STAT1 ELISA Kit
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(2 Publications)
- sELISA
Supplier Data
Sandwich ELISA - STAT1 (pY701) + total STAT1 ELISA Kit (AB126457)
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - STAT1 (pY701) + total STAT1 ELISA Kit (AB126457)
The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes to induce phosphorylation of STAT1. Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - STAT1 (pY701) + total STAT1 ELISA Kit (AB126457)
A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed using this phosphoELISA.
- WB
Supplier Data
Western blot - STAT1 (pY701) + total STAT1 ELISA Kit (AB126457)
A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed using Western Blot.
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- WB
Supplier Data
Western blot - STAT1 (pY701) + total STAT1 ELISA Kit (AB126457)
The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes to induce phosphorylation of STAT1. Serial dilutions of lysates were analyzed by Western blot. Immunoblots were incubated with anti-phospho-STAT1 (Tyr701).
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Reactivity data
Product details
ab126457 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated STAT1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human and Mouse phospho-STAT1 (Tyr701) and total STAT1 (help normalize the results of phospho-STAT1 from different cell lysate being compared). An anti-pan STAT1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and STAT1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho-STAT1 (Tyr701) antibody is added to detect phosphorylated STAT1. In the remaining wells, biotinylated anti-STAT1 antibody is used to detect pan STAT1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of STAT1 (Tyr701) or pan STAT1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
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Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.
Pathways
The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.
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Publications (2)
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The Journal of investigative dermatology 139:1583-1592 PubMed30703358
2019
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PLoS pathogens 11:e1005338 PubMed26709698
2015
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