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AB326399

STAT3 ELISA Kit, Chemiluminescent

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STAT3 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of STAT3 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 61.73 - 45000 pg/ml with a sensitivity of 16.9pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor

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Sandwich ELISA - STAT3 ELISA Kit, Chemiluminescent (AB326399)
  • sELISA

Lab

Sandwich ELISA - STAT3 ELISA Kit, Chemiluminescent (AB326399)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - STAT3 ELISA Kit, Chemiluminescent (AB326399)
  • sELISA

Lab

Sandwich ELISA - STAT3 ELISA Kit, Chemiluminescent (AB326399)

Standard curve comparison between the Chemiluminescent STAT3 SimpleStep ELISA and colorimetric STAT3 SimpleStep ELISA (ab264629) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - STAT3 ELISA Kit, Chemiluminescent (AB326399)
  • sELISA

Lab

Sandwich ELISA - STAT3 ELISA Kit, Chemiluminescent (AB326399)

Dynamic range comparison between the Chemiluminescent STAT3 SimpleStep ELISA, colorimetric STAT3 SimpleStep ELISA (ab264629), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - STAT3 ELISA Kit, Chemiluminescent (AB326399)
  • sELISA

Supplier Data

Sandwich ELISA - STAT3 ELISA Kit, Chemiluminescent (AB326399)

Interpolated concentrations of native STAT3 in human mouse and rat 100 ug/mL HeLa cell extract 250 ug/mL A431 cell extract 50 ug/mL mouse NIH3T3 cell extract and 50 ug/mL rat C6 cell extract.

The concentrations of STAT3 were measured in duplicate and interpolated from the STAT3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean STAT3 concentration was determined to be 10028.97 pg/mL in HeLa cell extract 3677.14 pg/mL in A431 cell extract 3906.20 pg/mL in mouse NIH3T3 cell extract and 5866.53 pg/mL in rat C6 cell extract.

Key facts

Detection method

Luminescent

Sample types

Cell Lysate

Reacts with

Human, Rat, Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 16.9 pg/mL

Range

61.73 - 45000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

STAT3 ELISA Kit, Chemiluminescent (ab326399) is a rapid single-wash sandwich 55-min ELISA to measure STAT3 in Cell Lysate with a sensitivity of 16.9 pg/mL.

How the assay works

STAT3 ELISA Kit, Chemiluminescent (ab326399) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

STAT3 (ab326399) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-STAT3 antibody [EPR21767-152] - BSA and Azide free (Capture) ab252594
- Anti-STAT3 antibody [EPR21767-108] - BSA and Azide free (Detector) ab252653
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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.7" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "100 - 109 %", "average": "= 104" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326399-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326399-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB326399-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X STAT3 Capture Antibody", "number":"AB326399-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326399-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X STAT3 Detector Antibody", "number":"AB326399-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326399-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326399-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326399-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326399-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326399-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"STAT3 Lyophilized Recombinant Protein", "number":"AB326399-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed : 10688651, PubMed : 12359225, PubMed : 12873986, PubMed : 15194700, PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18242580, PubMed : 18782771, PubMed : 22306293, PubMed : 23084476, PubMed : 28262505, PubMed : 32929201, PubMed : 38404237). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18782771, PubMed : 28262505, PubMed : 32929201). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed : 12359225). Activated by IL31 through IL31RA (PubMed : 15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg) : acetylation promotes its transcription activity and cell differentiation while deacetylation and oxidation of lysine residues by LOXL3 inhibits differentiation (PubMed : 28065600, PubMed : 28262505). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed : 17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed : 18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed : 23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC3 and NFATC4, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity).
See full target information STAT3
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websiteProtocolBooklet
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Product promise

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