AB126458

STAT3 (pY705) ELISA Kit

Name: STAT3 (pY705) ELISA Kit (ab126458) | Abcam
Brand: Abcam Limited
SKU: AB126458
Price: 780 USD
Availability: InStock

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(3 Publications)

STAT3 (pY705) ELISA Kit is a Semi-quantitative ELISA for the measurement of STAT3 (pY705) in Human, Mouse, Rat in Cell/Tissue Extracts samples.

View Alternative Names

APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor

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Sandwich ELISA - STAT3 (pY705) ELISA Kit (AB126458)

sELISA

Supplier Data

Sandwich ELISA - STAT3 (pY705) ELISA Kit (AB126458)

The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes. Serial dilutions of lysates were analyzed in this ELISA.

sELISA

Supplier Data

Sandwich ELISA - STAT3 (pY705) ELISA Kit (AB126458)

A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 20 min. Cell lysates were analyzed using this phosphoELISA.

sELISA

Supplier Data

Sandwich ELISA - STAT3 (pY705) ELISA Kit (AB126458)

A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

Supplier Data

Western blot - STAT3 (pY705) ELISA Kit (AB126458)

A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 20 min. Cell lysates were analyzed using Western Blot.

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Supplier Data

Western blot - STAT3 (pY705) ELISA Kit (AB126458)

The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes. Serial dilutions of lysates were analyzed by Western blot. Immunoblots were incubated with anti-phospho-STAT3 (Tyr705).

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Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse, Rat, Human

Assay type

Semi-quantitative

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

ab126458 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated STAT3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-STAT3 (Tyr705). An anti-pan STAT3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and STAT3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-STAT3 (Tyr705) antibody is used to detect phosphorylated STAT3 (Tyr705). After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STAT3 (Tyr705) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with STAT3 pY705 ELISA Kit (ab176654) from our SimpleStep ELISA^® range.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab126458-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB126458-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB126458-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB126458-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control: lyophilized powder from A431 cell lysate", "number":"AB126458-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"STAT3 Microplate (12 strips x 8 wells) coated with anti-pan STAT3 antibody", "number":"AB126458-CMP05", "productcode":"" }, { "size":"1 x 25 µL", "name":"2000x HRP-conjugated anti-rabbit IgG concentrate", "number":"AB126458-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB126458-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Detection Antibody STAT3 (Tyr705)", "number":"AB126458-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB126458-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 3 (STAT3) also called p-stat3 or phospho-STAT3 is a critical transcription factor involved in various cellular processes. The molecular weight of STAT3 is approximately 92 kDa. This protein is expressed broadly across many tissues and cell types. Mechanically upon cytokine or growth factor stimulation STAT3 undergoes phosphorylation resulting in dimerization. This phosphorylated form often referred to as phospho-Stat3 or p-STAT3 translocates to the nucleus where it binds specific DNA sequences to modulate gene transcription.

Biological function summary

STAT3 is an important player in regulating cell growth and apoptosis. It functions not only as a transcription factor but also as part of a larger protein complex that operates within the cell nucleus to influence gene expression. This multifaceted role enables STAT3 to control the expression of genes related to cell proliferation survival and differentiation impacting various biological processes. These functions highlight its importance in tissue homeostasis and response to extracellular signals.

Pathways

STAT3 is actively involved in the JAK-STAT signaling pathway a principal route for many cytokines and growth factors and the MAPK pathway. STAT3 interacts with proteins such as JAK kinases which phosphorylate Stat3 and initiate the STAT3 signaling cascade. Additionally it closely associates with the protein Raf1 in the MAPK pathway linking external signals to transcriptional responses. These pathways play a significant role in immune response inflammation and growth signaling.

Researchers have implicated STAT3 in cancer and autoimmune diseases. Persistent activation of STAT3 is often observed in various cancers including breast and lung cancer promoting oncogenesis through altered gene expression. Additionally abnormal STAT3 signaling is associated with autoimmune conditions such as rheumatoid arthritis. The interplay between STAT3 and proteins like IL-6 in these diseases highlights its potential as a therapeutic target for intervention.

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Product protocols

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Target data

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed : 10688651, PubMed : 12359225, PubMed : 12873986, PubMed : 15194700, PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18242580, PubMed : 18782771, PubMed : 22306293, PubMed : 23084476, PubMed : 28262505, PubMed : 32929201, PubMed : 38404237). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18782771, PubMed : 28262505, PubMed : 32929201). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed : 12359225). Activated by IL31 through IL31RA (PubMed : 15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg) : acetylation promotes its transcription activity and cell differentiation while deacetylation and oxidation of lysine residues by LOXL3 inhibits differentiation (PubMed : 28065600, PubMed : 28262505). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed : 17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed : 18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed : 23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC3 and NFATC4, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity).

See full target information STAT3 phospho Y705

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 34:549-563.e8 PubMed35298903

2022

Protein tyrosine phosphatase receptor δ serves as the orexigenic asprosin receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Ila Mishra,Wei Rose Xie,Juan C Bournat,Yang He,Chunmei Wang,Elizabeth Sabath Silva,Hailan Liu,Zhiqiang Ku,Yinghua Chen,Bernadette O Erokwu,Peilin Jia,Zhongming Zhao,Zhiqiang An,Chris A Flask,Yanlin He,Yong Xu,Atul R Chopra

Scientific reports 9:11628 PubMed31406162

2019

Danazol mediates collateral sensitivity via STAT3/Myc related pathway in multidrug-resistant cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Tzu Chang,Yu-Ning Teng,Kun-I Lin,Charles C N Wang,Susan L Morris-Natschke,Kuo-Hsiung Lee,Chin-Chuan Hung

JCI insight 2:e90196 PubMed28352655

2017

Panobinostat acts synergistically with ibrutinib in diffuse large B cell lymphoma cells with MyD88 L265P mutations.

Applications

ELISA

Species

Unspecified reactive species

Patrizia Mondello,Elliott J Brea,Elisa De Stanchina,Eneda Toska,Aaron Y Chang,Myles Fennell,Venkatraman Seshan,Ralph Garippa,David A Scheinberg,José Baselga,Hans-Guido Wendel,Anas Younes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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STAT3 (pY705) ELISA Kit

Sandwich ELISA - STAT3 (pY705) ELISA Kit (AB126458)

Sandwich ELISA - STAT3 (pY705) ELISA Kit (AB126458)

Sandwich ELISA - STAT3 (pY705) ELISA Kit (AB126458)

Western blot - STAT3 (pY705) ELISA Kit (AB126458)

Western blot - STAT3 (pY705) ELISA Kit (AB126458)

Key facts

Detection method

Sample types

Reacts with

Assay type

Assay time

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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