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AB176654

STAT3 pY705 ELISA Kit

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(1 Publication)

STAT3 pY705 ELISA Kit is a single-wash 90-min Simplestep used to quantify STAT3 pY705 with a sensitivity of 30 µg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
4 Images
ELISA - STAT3 pY705 ELISA Kit (AB176654)
  • ELISA

Supplier Data

ELISA - STAT3 pY705 ELISA Kit (AB176654)
Sandwich ELISA - STAT3 pY705 ELISA Kit (AB176654)
  • sELISA

Supplier Data

Sandwich ELISA - STAT3 pY705 ELISA Kit (AB176654)

Typical cell lysate dilution series.

Example of a typical STAT3 (pY705) cell lysate dilution series. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - STAT3 pY705 ELISA Kit (AB176654)
  • sELISA

Supplier Data

Sandwich ELISA - STAT3 pY705 ELISA Kit (AB176654)

STAT3 (pY705) phosphorylation in response to EGF treatment.

Induction of STAT3 (pY705) phosphorylation in A431 cells in response to EGF treatment. A431 cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of EGF before cell lysis. Data from quadruplicate measurements of STAT3 (pY705) are plotted and compared against total STAT3 protein levels.. Comparative STAT3 (pY705) and STAT3 (Total) data also shown by Western Blot.

Sandwich ELISA - STAT3 pY705 ELISA Kit (AB176654)
  • sELISA

Supplier Data

Sandwich ELISA - STAT3 pY705 ELISA Kit (AB176654)

Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1 x Cell Extraction Buffer PTR. Data from duplicate measurements of STAT3 (pY705) are normalized and plotted.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Suspension cells, Tissue Homogenate, Adherent cells

Reacts with

Human

Results type

Semi-Quantitative

Sensitivity

= 30 µg/mL

Range

30 - 3000 µg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

STAT3 pY705 ELISA Kit ab176654 is a rapid single-wash 90-min Other Immunoassay to measure STAT3 pY705 in cell culture extracts, suspension cells, tissue homogenate. This SimpleStep sensitivity is 30 µg/mL.

How the assay works

STAT3 pY705 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

STAT3 pY705 ELISA Kit ab176654 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "A431 Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "A431 Extract", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab176654-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB176654-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"STAT3 (pY705) Capture Antibody", "number":"AB176654-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Wash Buffer PT", "number":"AB176654-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB176654-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB176654-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB176654-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"STAT3 (pY705) Detector Antibody", "number":"AB176654-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution", "number":"AB176654-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized STAT3 Control Lysate", "number":"AB176654-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB176654-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 3 (STAT3) also called p-stat3 or phospho-STAT3 is a critical transcription factor involved in various cellular processes. The molecular weight of STAT3 is approximately 92 kDa. This protein is expressed broadly across many tissues and cell types. Mechanically upon cytokine or growth factor stimulation STAT3 undergoes phosphorylation resulting in dimerization. This phosphorylated form often referred to as phospho-Stat3 or p-STAT3 translocates to the nucleus where it binds specific DNA sequences to modulate gene transcription.
Biological function summary

STAT3 is an important player in regulating cell growth and apoptosis. It functions not only as a transcription factor but also as part of a larger protein complex that operates within the cell nucleus to influence gene expression. This multifaceted role enables STAT3 to control the expression of genes related to cell proliferation survival and differentiation impacting various biological processes. These functions highlight its importance in tissue homeostasis and response to extracellular signals.

Pathways

STAT3 is actively involved in the JAK-STAT signaling pathway a principal route for many cytokines and growth factors and the MAPK pathway. STAT3 interacts with proteins such as JAK kinases which phosphorylate Stat3 and initiate the STAT3 signaling cascade. Additionally it closely associates with the protein Raf1 in the MAPK pathway linking external signals to transcriptional responses. These pathways play a significant role in immune response inflammation and growth signaling.

Researchers have implicated STAT3 in cancer and autoimmune diseases. Persistent activation of STAT3 is often observed in various cancers including breast and lung cancer promoting oncogenesis through altered gene expression. Additionally abnormal STAT3 signaling is associated with autoimmune conditions such as rheumatoid arthritis. The interplay between STAT3 and proteins like IL-6 in these diseases highlights its potential as a therapeutic target for intervention.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 8:14733 PubMed30283098

2018

NF-κB-related decrease of glioma angiogenic potential by graphite nanoparticles and graphene oxide nanoplatelets.

Applications

Unspecified application

Species

Unspecified reactive species

Mateusz Wierzbicki,Ewa Sawosz,Barbara Strojny,Sławomir Jaworski,Marta Grodzik,André Chwalibog
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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