STAT3 (pY705 + Total) ELISA Kit is a single-wash 90-min Simplestep used to detect STAT3 (pY705 + Total). The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader
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- International journal of molecular sciences 23:2022Prophylactic Evidence of MSCs-Derived Exosomes in Doxorubicin/Trastuzumab-Induced Cardiotoxicity: Beyond Mechanistic Target of NRG-1/Erb Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNesrine Ebrahim,Hajir A Al Saihati,Ola Mostafa,Amira Hassouna,Sameh Abdulsamea,Eman Abd El Aziz M El Gebaly,Nashwa Hassan Abo-Rayah,Dina Sabry,Mohamed El-Sherbiny,Abdelmonem G Madboly,Noha Ibrahim Hussien,Raja El Hasnaoui Saadani,Hasnaa Ali Ebrahim,Omnia A M Badr,Nehal M Elsherbiny,Rabab F SalimPubMed 35682646
- Human reproduction (Oxford, England) 35:1781-17962020Periconceptional exposure to lopinavir, but not darunavir, impairs decidualization: a potential mechanism leading to poor birth outcomes in HIV-positive pregnancies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSmriti Kala,Caroline Dunk,Sebastian Acosta,Lena SerghidesPubMed 32712670
- Journal of dairy science 101:2588-26012017Effect of 17β-estradiol on milk production, hormone secretion, and mammary gland gene expression in dairy cows.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ J Tong,I M Thompson,X Zhao,P LacassePubMed 29274969
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay time
- 1h 30m
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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STAT3 (pY705 + Total) ELISA Kit is a single-wash 90-min Simplestep used to detect STAT3 (pY705 + Total). The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay time
- 1h 30m
- Assay Platform
- Microplate
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample STAT3 (Total) | n 6 | C.V. 2.9 |
Sample STAT3 (pY705) | n 6 | C.V. 3.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample STAT3 (Total) | n 3 | C.V. 7.4 |
Sample STAT3 (pY705) | n 3 | C.V. 5.4 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's STAT3 (pY705) and STAT3 (Total) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of STAT3 (pY705) and Total STAT3 protein in Human cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
As of October 2019, this kit was reformulated with new antibodies to maintain continued long term supply.
Estimated sensitivity: Phospho-STAT3 (Tyr705): 30 μg/mL (tested with HeLa), Total STAT3: 15 μg/mL (tested with A431)Range: Phospho-STAT3 (Tyr705): 20-2000 μg/mL, Total STAT3: 30-3000 μg/mL
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Signal Transducer and Activator of Transcription 3 (STAT3) also called p-stat3 or phospho-STAT3 is a critical transcription factor involved in various cellular processes. The molecular weight of STAT3 is approximately 92 kDa. This protein is expressed broadly across many tissues and cell types. Mechanically upon cytokine or growth factor stimulation STAT3 undergoes phosphorylation resulting in dimerization. This phosphorylated form often referred to as phospho-Stat3 or p-STAT3 translocates to the nucleus where it binds specific DNA sequences to modulate gene transcription.
- Biological function summary
STAT3 is an important player in regulating cell growth and apoptosis. It functions not only as a transcription factor but also as part of a larger protein complex that operates within the cell nucleus to influence gene expression. This multifaceted role enables STAT3 to control the expression of genes related to cell proliferation survival and differentiation impacting various biological processes. These functions highlight its importance in tissue homeostasis and response to extracellular signals.
- Pathways
STAT3 is actively involved in the JAK-STAT signaling pathway a principal route for many cytokines and growth factors and the MAPK pathway. STAT3 interacts with proteins such as JAK kinases which phosphorylate Stat3 and initiate the STAT3 signaling cascade. Additionally it closely associates with the protein Raf1 in the MAPK pathway linking external signals to transcriptional responses. These pathways play a significant role in immune response inflammation and growth signaling.
- Associated diseases and disorders
Researchers have implicated STAT3 in cancer and autoimmune diseases. Persistent activation of STAT3 is often observed in various cancers including breast and lung cancer promoting oncogenesis through altered gene expression. Additionally abnormal STAT3 signaling is associated with autoimmune conditions such as rheumatoid arthritis. The interplay between STAT3 and proteins like IL-6 in these diseases highlights its potential as a therapeutic target for intervention.
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4 product images
Sandwich ELISA - STAT3 (pY705 + Total) ELISA Kit (ab176666)
Typical cell lysate dilution series.
Example of a typical STAT3 (pY705) and STAT3 (Total) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - STAT3 (pY705 + Total) ELISA Kit (ab176666)
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of STAT3 (pY705) are normalized and plotted.
Sandwich ELISA - STAT3 (pY705 + Total) ELISA Kit (ab176666)
STAT3 (pY705) phosphorylation in response to EGF treatment.
Induction of STAT3 (pY705) phosphorylation in A431 cells in response to EGF treatment. A431 cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of EGF before cell lysis. Data from quadruplicate measurements of STAT3 (pY705) are plotted and compared against total STAT3 protein levels. Comparative STAT3 (pY705) and STAT3 (Total) data also shown by Western Blot.
Sandwich ELISA - STAT3 (pY705 + Total) ELISA Kit (ab176666)
Comparison of STAT3 expression in different cell lines.
Cell line analysis for Total STAT3 from 200 μg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
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