AB126459

STAT3 (pY705) + total STAT3 ELISA Kit

Name: STAT3 (pY705) + total STAT3 ELISA Kit (ab126459) | Abcam
Brand: Abcam Limited
SKU: AB126459
Price: 780 USD
Availability: InStock

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(7 Publications)

STAT3 (pY705) + total STAT3 ELISA Kit is a semi-quantitative sandwich ELISA kit to measure STAT3 (pY705) and total STAT3 in cell lysate samples.

- Colorimetric readout - works on any standard plate reader
- Semi quantitative: positive control included
- Measure numerous cell lysates at once

View Alternative Names

APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor

3 Images

Sandwich ELISA - STAT3 (pY705) + total STAT3 ELISA Kit (AB126459)

sELISA

Supplier Data

Sandwich ELISA - STAT3 (pY705) + total STAT3 ELISA Kit (AB126459)

A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

sELISA

Supplier Data

Sandwich ELISA - STAT3 (pY705) + total STAT3 ELISA Kit (AB126459)

A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 20 min. Cell lysates were analyzed using this phosphoELISA.

Supplier Data

Western blot - STAT3 (pY705) + total STAT3 ELISA Kit (AB126459)

A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 20 min. Cell lysates were analyzed using Western Blot.

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Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse, Rat, Human

Results type

Semi-Quantitative

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

STAT3 (pY705) + total STAT3 ELISA Kit (ab126459) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated STAT3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-STAT3 (Tyr705) and total STAT3 in human, mouse and rat cell lysates (help normalize the results of phospho-STAT3 from different cell lysate being compared). A pan STAT3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and STAT3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-STAT3 (Tyr705) is used to detect phosphorylated STAT3 or biotinylated anti-total-STAT3 is used to detect total STAT3. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STAT3 (Tyr705) or total STAT3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with STAT3 (pY705 + Total) ELISA Kit (ab176666) from our SimpleStep ELISA^® range.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab126459-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB126459-CMP07", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB126459-CMP11", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Detection Antibody STAT3 (Tyr705)", "number":"AB126459-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Detection Antibody STAT3", "number":"AB126459-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB126459-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"STAT3 Microplate (12 strips x 8 wells) coated with anti-pan STAT3 antibody", "number":"AB126459-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control: lyophilized powder from A431 cell lysate", "number":"AB126459-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"80x HRP-Streptavidin Concentrate", "number":"AB126459-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB126459-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB126459-CMP10", "productcode":"" }, { "size":"1 x 25 µL", "name":"2000x HRP-conjugated anti-rabbit IgG concentrate", "number":"AB126459-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 3 (STAT3) also called p-stat3 or phospho-STAT3 is a critical transcription factor involved in various cellular processes. The molecular weight of STAT3 is approximately 92 kDa. This protein is expressed broadly across many tissues and cell types. Mechanically upon cytokine or growth factor stimulation STAT3 undergoes phosphorylation resulting in dimerization. This phosphorylated form often referred to as phospho-Stat3 or p-STAT3 translocates to the nucleus where it binds specific DNA sequences to modulate gene transcription.

Biological function summary

STAT3 is an important player in regulating cell growth and apoptosis. It functions not only as a transcription factor but also as part of a larger protein complex that operates within the cell nucleus to influence gene expression. This multifaceted role enables STAT3 to control the expression of genes related to cell proliferation survival and differentiation impacting various biological processes. These functions highlight its importance in tissue homeostasis and response to extracellular signals.

Pathways

STAT3 is actively involved in the JAK-STAT signaling pathway a principal route for many cytokines and growth factors and the MAPK pathway. STAT3 interacts with proteins such as JAK kinases which phosphorylate Stat3 and initiate the STAT3 signaling cascade. Additionally it closely associates with the protein Raf1 in the MAPK pathway linking external signals to transcriptional responses. These pathways play a significant role in immune response inflammation and growth signaling.

Researchers have implicated STAT3 in cancer and autoimmune diseases. Persistent activation of STAT3 is often observed in various cancers including breast and lung cancer promoting oncogenesis through altered gene expression. Additionally abnormal STAT3 signaling is associated with autoimmune conditions such as rheumatoid arthritis. The interplay between STAT3 and proteins like IL-6 in these diseases highlights its potential as a therapeutic target for intervention.

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Product protocols

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Target data

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed : 10688651, PubMed : 12359225, PubMed : 12873986, PubMed : 15194700, PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18242580, PubMed : 18782771, PubMed : 22306293, PubMed : 23084476, PubMed : 28262505, PubMed : 32929201, PubMed : 38404237). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18782771, PubMed : 28262505, PubMed : 32929201). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed : 12359225). Activated by IL31 through IL31RA (PubMed : 15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg) : acetylation promotes its transcription activity and cell differentiation while deacetylation and oxidation of lysine residues by LOXL3 inhibits differentiation (PubMed : 28065600, PubMed : 28262505). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed : 17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed : 18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed : 23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC3 and NFATC4, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity).

See full target information STAT3 phospho Y705

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cachexia, sarcopenia and muscle 15:2447-2459 PubMed39210624

2024

Leptin signalling altered in infantile nephropathic cystinosis-related bone disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Wai W Cheung,Ping Zhou,Ronghao Zheng,Arieh Gertler,Eduardo A Oliveira,Robert H Mak

Pharmaceutics 15: PubMed36839928

2023

Chitosan Surface-Modified PLGA Nanoparticles Loaded with Cranberry Powder Extract as a Potential Oral Delivery Platform for Targeting Colon Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mona M Mostafa,Maha M Amin,Mohamed Y Zakaria,Mohammed Abdalla Hussein,Marium M Shamaa,Shady M Abd El-Halim

Journal of biochemical and molecular toxicology 35:e22924 PubMed34605108

2021

Thymoquinone upregulates miR-125a-5p, attenuates STAT3 activation, and potentiates doxorubicin antitumor activity in murine solid Ehrlich carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hebatallah H Atteia,Manar H Arafa,Nanies S Mohammad,Dalia M Amin,Amr T Sakr

Molecular medicine reports 22:2052-2062 PubMed32582964

2020

Statin rosuvastatin inhibits apoptosis of human coronary artery endothelial cells through upregulation of the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kuijing Wang,Bo Li,Yuanyuan Xie,Nan Xia,Minghui Li,Guang Gao

The Journal of investigative dermatology 139:1583-1592 PubMed30703358

2019

Chronic Inflammation in Response to Injury: Retention of Myeloid Cells in Injured Tissue Is Driven by Myeloid Cell Intrinsic Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Tanja Torbica,Kate Wicks,Takahiro Umehara,Lale Gungordu,Salma Alrdahe,Kelly Wemyss,John R Grainger,Kimberly A Mace

Scientific reports 8:15917 PubMed30374077

2018

Lipopolysaccharide shock reveals the immune function of indoleamine 2,3-dioxygenase 2 through the regulation of IL-6/stat3 signalling.

Applications

Unspecified application

Species

Mouse

Yasuko Yamamoto,Wakana Yamasuge,Shinjiro Imai,Kazuo Kunisawa,Masato Hoshi,Hidetsugu Fujigaki,Akihiro Mouri,Toshitaka Nabeshima,Kuniaki Saito

Scientific reports 5:17567 PubMed26631552

2015

A preliminary study of ALPPS procedure in a rat model.

Applications

Unspecified application

Species

Rat

Huawen Shi,Guangchao Yang,Tongsen Zheng,Jiabei Wang,Lulu Li,Yingjian Liang,Changming Xie,Dalong Yin,Boshi Sun,Jing Sun,Huanlai Wang,Shangha Pan,Hongchi Jiang,WanYee Lau,Lianxin Liu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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STAT3 (pY705) + total STAT3 ELISA Kit

Sandwich ELISA - STAT3 (pY705) + total STAT3 ELISA Kit (AB126459)

Sandwich ELISA - STAT3 (pY705) + total STAT3 ELISA Kit (AB126459)

Western blot - STAT3 (pY705) + total STAT3 ELISA Kit (AB126459)

Key facts

Detection method

Sample types

Reacts with

Results type

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise