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AB126427

STAT3 (Tyr705) In-Cell ELISA Kit

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(1 Publication)

STAT3 (Tyr705) In-Cell ELISA Kit is a Cell-based (qualitative) ELISA for the measurement of STAT3 (Tyr705) In-Cell in Human, Mouse, Rat in Cell Samples samples.

View Alternative Names

APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor

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In-Cell ELISA - STAT3 (Tyr705) In-Cell ELISA Kit (AB126427)
  • In-Cell ELISA

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In-Cell ELISA - STAT3 (Tyr705) In-Cell ELISA Kit (AB126427)

A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37°C.

In-Cell ELISA - STAT3 (Tyr705) In-Cell ELISA Kit (AB126427)
  • In-Cell ELISA

Unknown

In-Cell ELISA - STAT3 (Tyr705) In-Cell ELISA Kit (AB126427)

A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37°C.

In-Cell ELISA - STAT3 (Tyr705) In-Cell ELISA Kit (AB126427)
  • In-Cell ELISA

Lab

In-Cell ELISA - STAT3 (Tyr705) In-Cell ELISA Kit (AB126427)

A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37°C.

Western blot - STAT3 (Tyr705) In-Cell ELISA Kit (AB126427)
  • WB

Supplier Data

Western blot - STAT3 (Tyr705) In-Cell ELISA Kit (AB126427)

Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells. Phospho-Stat3 (Tyr705) and Anti-Stat3 antibodies were used in both detection assays.

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Key facts

Detection method

Colorimetric

Sample types

Adherent cells

Reacts with

Mouse, Rat, Human

Assay type

Cell-based (qualitative)

Assay time

5h 10m

Assay Platform

Microplate

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Reactivity data

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Product details

ab126427 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of STAT3 (Tyr705) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines. By determining STAT3 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.

In the STAT3 (Tyr705) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti-Phospho-STAT3 (Tyr705) or Anti-STAT3 is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-mouse IgG is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 3 (STAT3) also called p-stat3 or phospho-STAT3 is a critical transcription factor involved in various cellular processes. The molecular weight of STAT3 is approximately 92 kDa. This protein is expressed broadly across many tissues and cell types. Mechanically upon cytokine or growth factor stimulation STAT3 undergoes phosphorylation resulting in dimerization. This phosphorylated form often referred to as phospho-Stat3 or p-STAT3 translocates to the nucleus where it binds specific DNA sequences to modulate gene transcription.
Biological function summary

STAT3 is an important player in regulating cell growth and apoptosis. It functions not only as a transcription factor but also as part of a larger protein complex that operates within the cell nucleus to influence gene expression. This multifaceted role enables STAT3 to control the expression of genes related to cell proliferation survival and differentiation impacting various biological processes. These functions highlight its importance in tissue homeostasis and response to extracellular signals.

Pathways

STAT3 is actively involved in the JAK-STAT signaling pathway a principal route for many cytokines and growth factors and the MAPK pathway. STAT3 interacts with proteins such as JAK kinases which phosphorylate Stat3 and initiate the STAT3 signaling cascade. Additionally it closely associates with the protein Raf1 in the MAPK pathway linking external signals to transcriptional responses. These pathways play a significant role in immune response inflammation and growth signaling.

Researchers have implicated STAT3 in cancer and autoimmune diseases. Persistent activation of STAT3 is often observed in various cancers including breast and lung cancer promoting oncogenesis through altered gene expression. Additionally abnormal STAT3 signaling is associated with autoimmune conditions such as rheumatoid arthritis. The interplay between STAT3 and proteins like IL-6 in these diseases highlights its potential as a therapeutic target for intervention.
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Product protocols

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Target data

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed : 10688651, PubMed : 12359225, PubMed : 12873986, PubMed : 15194700, PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18242580, PubMed : 18782771, PubMed : 22306293, PubMed : 23084476, PubMed : 28262505, PubMed : 32929201, PubMed : 38404237). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18782771, PubMed : 28262505, PubMed : 32929201). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed : 12359225). Activated by IL31 through IL31RA (PubMed : 15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg) : acetylation promotes its transcription activity and cell differentiation while deacetylation and oxidation of lysine residues by LOXL3 inhibits differentiation (PubMed : 28065600, PubMed : 28262505). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed : 17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed : 18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed : 23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC3 and NFATC4, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity).
See full target information STAT3 phospho Y705
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 21:161 PubMed34078370

2021

Cryptolepine inhibits hepatocellular carcinoma growth through inhibiting interleukin-6/STAT3 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Seth A Domfeh,Patrick W Narkwa,Osbourne Quaye,Kwadwo A Kusi,Gordon A Awandare,Charles Ansah,Alimatu Salam,Mohamed Mutocheluh
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