AB288318

Substance P ELISA Kit

Name: Substance P ELISA Kit (ab288318) | Abcam
Brand: Abcam Limited
SKU: AB288318
Price: 730 USD
Availability: InStock

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(2 Publications)

Substance P ELISA Kit is a competitive ELISA designed to quantify Substance P with a sensitivity of 5.3 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 9.8 - 10000 pg/ml

View Alternative Names

NKA, NKNA, TAC2, TAC1, Protachykinin-1, PPT

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant, Serum

Assay type

Competitive

Sensitivity

= 5.3 pg/mL

Range

9.8 - 10000 pg/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Substance P in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Substance P in biological fluids.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Substance P antigen and a polyclonal rabbit antibody specific to Substance P. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Substance P captured in the plate.

Substance P is a undecapeptide that displays a number of biological activities. The peptide was first discovered in 1931 by von Euler and Gaddum. They reported that extracts of equine brain and intestine contained a hypotensive and spasmogenic factor. The preparation, termed preparation P, was later found to be proteinaceous. The isolation and characterization of Substance P was carried out by Leeman's group in 1970. The structure is shown below.

Substance P: H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2

Substance P is synthesized in the ribosomes as a larger protein and then enzymatically converted into the active peptide. The peptide is widely distributed in the peripheral and central nervous systems of vertebrates, where it is thought to act as a neurotransmitter. In the peripheral system, Substance P is localized in the primary sensory neurons and neurons intrinsic to the gastrointestinal tract.

Cross Reactivity

Compound	% Cross Reactivity
Substance P	100
Substance P (4-11)	>100
Substance P (7-11)	>100
Substance P (3-11)	66
Physalaemin	50
Eledoisin	<0.001
α-Neurokinin	<0.001
β-Neurokinin	<0.001
Somatostatin	<0.001
Substance P (1-4)	<0.001

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Recovery

[ { "sample": "Serum", "range": null, "average": "= 97.7" }, { "sample": "Urine", "range": null, "average": "= 105.8" }, { "sample": "Saliva", "range": null, "average": "= 108.3" }, { "sample": "Heparin Plasma", "range": null, "average": "= 109.3" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 81" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288318-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB288318-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB288318-CMP09", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB288318-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"Substance P Antibody", "number":"AB288318-CMP05", "productcode":"" }, { "size":"1 x 500 µL", "name":"Substance P Standard", "number":"AB288318-CMP07", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB288318-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB288318-CMP03", "productcode":"" }, { "size":"1 x 27 mL", "name":"Assay Buffer", "number":"AB288318-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"Substance P Alkaline Phosphatase Conjugate", "number":"AB288318-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Substance P also known as neurokinin 1 (NK1) receptor agonist is a neuropeptide with molecular mass approximately 1.3 kDa. Substance P exists mainly in the central nervous system and peripheral nervous system. This peptide performs mechanical roles by interacting with its receptor the NK1 receptor which is widespread in neurons and various tissues. It is important for processing pain signals and mood regulation. Substance P belongs to the family of tachykinins which are characterized by the common C-terminal sequence.

Biological function summary

This peptide acts as a neurotransmitter and neuromodulator in the body. Its functions are significant in the transmission of pain information into the central nervous system where it acts independently rather than being part of a larger protein complex. Additionally Substance P contributes to the regulation of inflammatory processes which impacts immune response. This peptide is not only important for pain perception but also participates in regulating emotional responses and stress.

Pathways

Substance P has a major role in the neurokinin signaling pathway. Within this pathway it interacts with other proteins such as calcitonin gene-related peptide (CGRP) enhancing its function in pain transmission and modulation. Additionally Substance P is part of the MAPK/ERK pathway where it influences cell proliferation and survival although its primary actions focus on signaling through the NK1 receptor.

Substance P is connected to conditions such as chronic pain and depression. By interacting with proteins like the NK1 receptor it is implicated in the sensation and perception of pain leading to an increased understanding of chronic pain mechanisms. Substance P also relates to inflammatory diseases with its involvement in pathways that influence inflammation and could serve as targets for therapeutic intervention in disorders like arthritis. Anti-substance therapeutics aim to modulate its effects to treat these conditions.

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Product protocols

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Target data

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.. Substance P. Is a ligand for TACR1, and triggers G protein-coupled receptor signaling via activation of phosphatidylinositol hydrolysis by phospholipase C. Substance P binding to TACR1 also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP) (By similarity). Is also a TACR3 agonist with low receptor affinity (PubMed : 37391393). Basic secretagogue neuropeptide released from the terminals of specific sensory nerves (PubMed : 30686732). Acts as a ligand for MRGPRX2 receptor in mast cells, initiating a signaling that mediates neurogenic inflammation and pain (PubMed : 30686732, PubMed : 34789875). Neurogenic inflammation includes mast cell activation, recruitment of immune cells and release of inflammatory mediators, such as cytokines and chemokines (By similarity). The inflammatory response can then activate or sensitize nociceptors, promoting pain (By similarity).. Neurokinin A. Is a ligand for TACR2, and triggers G protein-coupled receptor signaling via activation of G(q) and phosphatidylinositol hydrolysis by phospholipase C (PubMed : 35882833). Binding to TACR2 also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP).

See full target information TAC1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 18:1583908 PubMed40453756

2025

Bidirectional substance P signaling between periodontal ligament fibroblasts and sensory neurons under mechanical stress.

Applications

Unspecified application

Species

Unspecified reactive species

Judit Symmank,Lara Löffler,Ulrike Schulze-Späte,Collin Jacobs

International journal of stem cells 16:269-280 PubMed37385635

2023

Peripheral Neuron-Organoid Interaction Induces Colonic Epithelial Differentiation via Non-Synaptic Substance P Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Young Hyun Che,In Young Choi,Chan Eui Song,Chulsoon Park,Seung Kwon Lim,Jeong Hee Kim,Su Haeng Sung,Jae Hoon Park,Sun Lee,Yong Jun Kim

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Substance P ELISA Kit

Key facts

Detection method

Sample types

Assay type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise