AB223136

Swine Haptoglobin ELISA Kit

Name: Swine Haptoglobin ELISA Kit (ab223136) | Abcam
Brand: Abcam Limited
SKU: AB223136

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(1 Publication)

Swine Haptoglobin ELISA Kit is a Sandwich ELISA for the measurement of Swine Haptoglobin in Pig in Biofluids samples.

View Alternative Names

Haptoglobin, HP

1 Images

Sandwich ELISA - Swine Haptoglobin ELISA Kit (AB223136)

sELISA

Supplier Data

Sandwich ELISA - Swine Haptoglobin ELISA Kit (AB223136)

Example of Swine Haptoglobin standard curve

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Serum, Urine

Reacts with

Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.51 ng/mL

Range

0.938 - 60 ng/mL

Assay time

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

The Swine Haptoglobin ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab223136) is designed for detection of swine haptoglobin in plasma, serum, urine, and cell culture samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures swine haptoglobin in approximately 4 hours. A polyclonal antibody specific for swine haptoglobin has been pre-coated onto a 96-well microplate with removable strips. Swine haptoglobin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for swine haptoglobin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Haptoglobin (HP, zonulin) is a plasma protein with hemoglobin-binding capacity and a plasma glycoprotein that forms a stable complex with hemoglobin to aid the recycling of heme iron.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab223136-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB223136-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB223136-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB223136-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Swine Haptoglobin Standard", "number":"AB223136-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Swine Haptoglobin Antibody", "number":"AB223136-CMP03", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB223136-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Swine Haptoglobin coated Microplate (12 x 8 wells)", "number":"AB223136-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB223136-CMP08", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB223136-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.

Biological function summary

Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.

Pathways

The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.

Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.

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Product protocols

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Target data

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).

See full target information HP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 13: PubMed40006753

2025

Outer Membrane Proteins as Vaccine Targets Against in Piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Kara L Aves,Ana H Fresno,Sajid Nisar,Mauro M Saraiva,Nicole B Goecke,Adam F Sander,Morten A Nielsen,John E Olsen,Priscila R Guerra

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Swine Haptoglobin ELISA Kit

Sandwich ELISA - Swine Haptoglobin ELISA Kit (AB223136)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise