Swine Haptoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Swine Haptoglobin in Pig in Citrate plasma, Serum, Urine samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Pig
- Range
- 0.938 - 60 ng/mL
- Assay time
- 4h
- Sensitivity
- = 0.51 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
Alternative names
Haptoglobin, HP
What's included?
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Swine Haptoglobin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Swine Haptoglobin in Pig in Citrate plasma, Serum, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Pig
- Range
- 0.938 - 60 ng/mL
- Assay time
- 4h
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.51 ng/mL
- General recovery
- 97%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 0 | mean - | SD - | C.V. 10.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 0 | mean - | SD - | C.V. 5.4 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
The Swine Haptoglobin ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab223136) is designed for detection of swine haptoglobin in plasma, serum, urine, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures swine haptoglobin in approximately 4 hours. A polyclonal antibody specific for swine haptoglobin has been pre-coated onto a 96-well microplate with removable strips. Swine haptoglobin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for swine haptoglobin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Haptoglobin (HP, zonulin) is a plasma protein with hemoglobin-binding capacity and a plasma glycoprotein that forms a stable complex with hemoglobin to aid the recycling of heme iron.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.
- Biological function summary
Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.
- Pathways
The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.
- Associated diseases and disorders
Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.
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Sandwich ELISA - Swine Haptoglobin ELISA Kit (ab223136)
Example of Swine Haptoglobin standard curve
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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