JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277088

Syntaxin 1a ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Syntaxin 1a ELISA Kit is a single-wash 90-min Simplestep used to quantify Syntaxin 1a with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

STX1, STX1A, Syntaxin-1A, Neuron-specific antigen HPC-1

2 Images
Sandwich ELISA - Syntaxin 1a ELISA Kit (AB277088)
  • sELISA

Supplier Data

Sandwich ELISA - Syntaxin 1a ELISA Kit (AB277088)

Interpolated concentrations of native Syntaxin 1a in 80 μg/mL SHSY-5Y cell extract, 20 μg/mL mouse brain tissue extract and 15 μg/mL rat brain tissue extract.

The concentrations of Syntaxin 1a were measured in duplicate and interpolated from the Syntaxin 1a standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Syntaxin 1a concentration was determined to be 3,353.80 pg/mL in SHSY-5Y cell extract, 2,463.89 pg/mL in mouse brain tissue extract and 3,289.96 pg/mL in rat brain tissue extract.

Sandwich ELISA - Syntaxin 1a ELISA Kit (AB277088)
  • sELISA

Supplier Data

Sandwich ELISA - Syntaxin 1a ELISA Kit (AB277088)

Example Syntaxin 1a standard curve in 1X Cell Extraction Buffer PTR.

The Syntaxin 1a standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Mouse, Rat, Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 36 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB233626

Mouse NSE / Neuron-specific Enolase ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (AB233626)

  • 0
  • 0
Assay Kits

AB277713

Mouse PARK7 (DJ1) ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse PARK7 (DJ1) ELISA Kit (AB277713)

  • 0
  • 0
Primary Antibodies

AB272736

Anti-Syntaxin 1a antibody [EPR23457-15]

BOND RX™ Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] (AB272736)

  • 0
  • 0
Assay Kits

AB285309

Mouse Superoxide Dismutase 1 ELISA Kit

Sandwich ELISA - Mouse Superoxide Dismutase 1 ELISA Kit (AB285309)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Syntaxin 1a ELISA kit (ab277088) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human/Mouse/Rat Syntaxin 1a protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Syntaxin 1a with 36 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Syntaxin 1a is part of the SNARE complex, which plays a critical role in neurotransmission. With its partners, SNAP25 and VAMP2, it is responsible for regulating calcium ion channels and for fusion of synaptic vesicles with the presynaptic membrane. This protein shares homology across Mammalia, and therefore is expected to cross-react with many species. The antibodies in this kit were raised against the human full-length recombinant protein.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]
style
left-column

Recovery

[ { "sample": "Tissue Extracts", "range": "111 - 116 %", "average": "= 113" }, { "sample": "Cell Lysate", "range": "88 - 104 %", "average": "= 97" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277088-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277088-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB277088-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277088-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277088-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Syntaxin 1a Lyophilized Recombinant Protein", "number":"AB277088-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Syntaxin 1a Capture Antibody", "number":"AB277088-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB277088-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277088-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277088-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Syntaxin 1a Detector Antibody", "number":"AB277088-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277088-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 1a also known as STX1A is an essential protein in cellular mechanisms involved in neurotransmitter release. This protein weighing approximately 33 kDa plays a direct role in synaptic vesicle fusion through its function as a member of the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein Receptor) complex. Syntaxin 1a is highly expressed in the brain especially in areas like the hippocampus and cerebral cortex where it facilitates rapid neurotransmission important for communication between neurons.
Biological function summary

The involvement of Syntaxin 1a in synaptic transmission is significant. It forms a complex with other proteins such as SNAP-25 and VAMP enabling the docking and fusion of synaptic vesicles with the presynaptic membrane. This action results in the release of neurotransmitters into the synaptic cleft which then bind to receptors on the postsynaptic neuron to propagate neural signals. Syntaxin 1a ensures efficient synaptic activity and is indispensable for maintaining synaptic plasticity which is critical for learning and memory.

Pathways

Syntaxin 1a is pivotal in the regulation of neurotransmitter release within the synaptic vesicle cycle pathway. This pathway includes the assembly and disassembly of the SNARE complex which are processes vital for neurotransmitter exocytosis. Syntaxin 1a engages closely with proteins like synaptotagmin acting as calcium sensors and Munc18 which helps in initiating SNARE assembly. These interactions highlight the careful orchestration of vesicle trafficking and fusion events necessary for effective synaptic communication.

Dysfunctions in Syntaxin 1a are linked to neurological conditions such as epilepsy and autism spectrum disorders. Alterations or mutations in Syntaxin 1a can lead to improper synaptic vesicle fusion and disrupted neurotransmitter release which are characteristics of these disorders. Additionally Syntaxin 1a interacts with proteins like Munc18-1 whose mutations are associated with similar synaptic transmission deficiencies further implicating the involvement of Syntaxin 1a in these pathologies.
style
left-column

Product protocols

style
left-column

Target data

Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed : 26635000). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm (PubMed : 23091057). Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (By similarity).
See full target information STX1A
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com