Testosterone ELISA kit is a competitive ELISA designed to quantify human testosterone in serum, plasma (citrate, heparin), and urine with a sensitivity of 0.07 ng/mL.
- Wide dynamic range – quantifies 0.2–16 ng/mL
- Designed to measure bound and free testosterone
- High recovery rate – achieves 105.5% general recovery
- Cited in over 55 publications
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- Cells 12:2023Effect of Low Testosterone Levels on the Expression of Proliferator-Activated Receptor Alpha in Female Patients with Primary Biliary Cholangitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnieszka Kempińska-Podhorodecka,Joanna Abramczyk,Eliza Cielica,Bartosz Huła,Hanna Maciejowska,Jesus Banales,Piotr Milkiewicz,Małgorzata MilkiewiczPubMed 37759496
- Scientific reports 13:143002023Effect of exercise on the hypothalamic-pituitary-gonadal axis in a rat model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEman Y Khairy,Ola A SalamaPubMed 37653057
- Biomedicines 11:2023Effects on Bone and Muscle upon Treadmill Interval Training in Hypogonadal Male Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIoannis Stratos,Ingmar Rinas,Konrad Schröpfer,Katharina Hink,Philipp Herlyn,Mario Bäumler,Tina Histing,Sven Bruhn,Brigitte Müller-Hilke,Michael D Menger,Brigitte Vollmar,Thomas MittlmeierPubMed 37239040
- Biology of reproduction 108:945-9592023Glucagon-like peptide-1 receptor agonists decrease hyperinsulinemia and hyperandrogenemia in dehydroepiandrosterone-induced polycystic ovary syndrome mice and are associated with mitigating inflammation and inducing browning of white adipose tissue†.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYahui Zhang,Yi Lin,Guoqiang Li,Yuan Yuan,Xuejiao Wang,Na Li,Chuanhao Xiong,Yueying Yang,Yuhang Ma,Zhijian Zhang,Xiaoying DingPubMed 36930063
- iScience 25:1053002022Testosterone protects mice against zika virus infection and suppresses the inflammatory response in the brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBohan Zheng,Jiajun Sun,Haoran Luo,Ling'en Yang,Qi Li,Luping Zhang,Youhui Si,Shengbo Cao,Jing YePubMed 36304103
- Molecular medicine reports 26:2022CTBP1‑AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingxiao Wen,Xiaoqing Dou,Shuzhen Zhang,Bin Wang,Jiangyan Xu,Wenchao Zhang,Feifei WangPubMed 35656892
- Frontiers in endocrinology 13:8715482022ICA II Alleviates Testicular Torsion Injury by Dampening the Oxidative and Inflammatory Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAni Chi,Bicheng Yang,Xiaohui Cao,Zhenqing Wang,Hanchao Liu,Hao Dai,Chunhua Deng,Min ZhangPubMed 35634492
- Nature 602:112-1162022Androgens increase excitatory neurogenic potential in human brain organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIva Kelava,Ilaria Chiaradia,Laura Pellegrini,Alex T Kalinka,Madeline A LancasterPubMed 35046577
- The Journal of endocrinology 251:69-812021Prenatal androgen excess impairs beta-cell function by decreased sirtuin 3 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhou,Min Gong,Yingfei Lu,Jianquan Chen,Rong JuPubMed 34370691
- Cell reports 36:1095792021Skin exposure to UVB light induces a skin-brain-gonad axis and sexual behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoma Parikh,Eschar Sorek,Shivang Parikh,Keren Michael,Lior Bikovski,Sagi Tshori,Galit Shefer,Shira Mingelgreen,Taiba Zornitzki,Hilla Knobler,Gabriel Chodick,Mariya Mardamshina,Arjan Boonman,Noga Kronfeld-Schor,Hadas Bar-Joseph,Dalit Ben-Yosef,Hadar Amir,Mor Pavlovsky,Hagit Matz,Tom Ben-Dov,Tamar Golan,Eran Nizri,Daphna Liber,Yair Liel,Ronen Brenner,Yftach Gepner,Orit Karnieli-Miller,Rina Hemi,Ruth Shalgi,Tali Kimchi,Ruth Percik,Aron Weller,Carmit LevyPubMed 34433056
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Heparin Plasma, Serum, Urine
- Assay type
- Competitive
- Reactive species
- Human
- Range
- 0.2 - 16 ng/mL
- Sensitivity
- = 0.07 ng/mL
Product summary
Human testosterone ELISA kit (colorimetric) offers:
Wide dynamic range – quantifies 0.2–16 ng/mL
Assay platform – microplate for easy setup and reliable results
High recovery rate – achieves 105.5% general recovery
Cited in over 55 publications
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Alternative names
17b hydroxy 4 androsten 3 one, 17鑴-hydroxyandrost-4-ene-3-one
What's included?
Recommended products
Testosterone ELISA kit is a competitive ELISA designed to quantify human testosterone in serum, plasma (citrate, heparin), and urine with a sensitivity of 0.07 ng/mL.
- Wide dynamic range – quantifies 0.2–16 ng/mL
- Designed to measure bound and free testosterone
- High recovery rate – achieves 105.5% general recovery
- Cited in over 55 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Heparin Plasma, Serum, Urine
- Assay type
- Competitive
- Reactive species
- Human
- Range
- 0.2 - 16 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 0.07 ng/mL
- General recovery
- 105.5%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 48 | mean - | SD - | C.V. <= 5.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 48 | mean - | SD - | C.V. <= 10.5 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Testosterone ELISA Kit ab108666 is a competitive ELISA kit to measure human Testosterone (17β-Hydroxy-4-androstene-3-one) in serum, plasma (citrate, heparin), and urine with a sensitivity of 0.07 ng/mL.
How the assay works
Testosterone ELISA Kit ab108666 is a competitive ELISA. There are 2 types of competitive ELISAs:
- antigen down: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
- antibody down: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Testosterone ELISA Kit ab108666 protocol summary
- Add standards or samples to the wells, incubate 2 hrs
- Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
- Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
- Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
- Add 50 µl of Stop Solution per well. Read at 450 nm immediately
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Testosterone also known as 17β-hydroxyandrost-4-en-3-one is a steroid hormone with a molecular mass of approximately 288.42 Da. It is primarily synthesized in the testes but also in smaller amounts in the ovaries and adrenal glands. Mechanically testosterone operates by binding to the androgen receptor a member of the nuclear receptor family which then modulates gene expression. Its synthesis is stimulated by luteinizing hormone (LH) which is released from the anterior pituitary gland.
- Biological function summary
Testosterone plays an important role in the development of male secondary sexual characteristics such as increased muscle and bone mass and the growth of body hair. It's also part of the steroid hormone biosynthesis pathway influencing the production of estradiol through the aromatase enzyme. Testosterone contributes to the maintenance of reproductive tissues and is important for the modulation of mood and cognitive functions.
- Pathways
Testosterone is integral to the HPG (hypothalamic-pituitary-gonadal) axis where it exerts feedback control by inhibiting the secretion of gonadotropin-releasing hormone (GnRH) and LH. Moreover testosterone is essential in the androgen receptor signaling pathway. This pathway involves other proteins like dihydrotestosterone (DHT) a potent androgen converted from testosterone via the 5α-reductase enzyme.
- Associated diseases and disorders
Low levels of testosterone commonly known as 'Low T' are linked to conditions like hypogonadism and certain metabolic syndromes. Testosterone levels can influence the risk of osteoporosis as sustained low levels reduce bone density. Additionally increased or decreased testosterone levels can pose risks to cardiovascular health with the target interacting with proteins such as sex hormone-binding globulin (SHBG) in these contexts.
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2 product images
Competitive ELISA - Testosterone ELISA Kit (ab108666)
Representative Standard Curve using ab108666
Competitive ELISA - Testosterone ELISA Kit (ab108666)
Testosterone measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.
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Product protocols
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