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AB133022

Thromboxane B2 ELISA Kit

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(6 Publications)

Thromboxane B2 ELISA Kit is a competitive ELISA designed to quantify Thromboxane B2 with a sensitivity of 10.54 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 13.7 - 10000 pg/ml

View Alternative Names

T box 2, T-box protein 2, T-box transcription factor TBX2, Thromboxane B2

1 Images
Competitive ELISA - Thromboxane B2 ELISA Kit (AB133022)
  • cELISA

Supplier Data

Competitive ELISA - Thromboxane B2 ELISA Kit (AB133022)

Representative Standard Curve using ab133022.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Serum, Tissue Culture Media, Cell culture supernatant, Cell Lysate, Plasma, Platelets, Whole Blood, Liver Extract, Heart Extract

Reacts with

Human

Assay type

Competitive

Sensitivity

= 10.54 pg/mL

Range

13.7 - 10000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Thromboxane B2 (TXB2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thromboxane B2 in biological fluids.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-TXB2 antigen and a rabbit polyclonal antibody specific to TXB2. After incubation the excess reagents are washed away and pNpp substrate is added. After a short incubation the enzyme reaction is stopped and a yellow color is generated. The intensity of the yellow coloration is inversely proportional to the amount of TXB2 captured in the plate.

Thromboxane A2 (TXA2) is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a half life of only 37 seconds under physiological conditions. Thromboxane B2 (TXB2) is the stable product of the non-enzymatic hydration of TXA2, thus the production of TXA2 in vivo is typically monitored by measurement of TXB2 and its metabolite 2,3-dinor TXB2. 11-dehydro-TXB2 is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma produced by its dehydrogenation by the enzyme 11-OH-dehydrogenase.

Cross Reactivity

Compound Cross Reactivity
TXB2 100%
2,3-dinorTXB2 7.1%
11-dehydro TXB2 0.4%
6-keto-PGF1α 0.23%
PGD2 <0.1%
PGE2 <0.1%
PGF1α <0.1%
PGF2α <0.1%
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Precision

[ { "reproducibilityType": "Inter", "sample": "Low", "replicates": 8, "mean": "44 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 7.6" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 8, "mean": "371 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 3.6" }, { "reproducibilityType": "Inter", "sample": "High", "replicates": 8, "mean": "3053 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 24, "mean": "2605 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 1.6" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 24, "mean": "759 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 3.6" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 24, "mean": "1283 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 4" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 113" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 104.7" }, { "sample": "Urine", "range": null, "average": "= 102.2" }, { "sample": "Saliva", "range": null, "average": "= 98.3" }, { "sample": "Heparin Plasma", "range": null, "average": "= 111" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133022-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB133022-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB133022-CMP09", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133022-CMP08", "productcode":"" }, { "size":"1 x 500 µL", "name":"Thromboxane B2 Standard", "number":"AB133022-CMP05", "productcode":"" }, { "size":"1 x 27 mL", "name":"Assay Buffer", "number":"AB133022-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133022-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133022-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"Thromboxane B2 Alkaline Phosphatase Conjugate", "number":"AB133022-CMP07", "productcode":"" }, { "size":"1 x 5 mL", "name":"Rabbit Polyclonal Thromboxane B2 Antibody", "number":"AB133022-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TXB2 also known as Thromboxane B2 is a stable metabolite of Thromboxane A2. It has a molecular mass of about 368.45 g/mol. TXB2 acts mechanically as a product of arachidonic acid metabolism and is synthesized through the cyclooxygenase (COX) pathway. It is not active itself but serves as a reliable marker for the production of Thromboxane A2. This target is expressed in various tissues especially those involved in inflammation and hemostasis.
Biological function summary

Thromboxane B2 plays a role in platelet aggregation and vasoconstriction. It is part of the Thromboxane complex which includes other prostaglandins and their metabolites. The biological actions of TXB2 demonstrate its involvement in the modulation of blood flow and blood clot formation. As a non-active but stable marker TXB2 offers insights into the activity and levels of its more unstable precursor impacting processes related to vascular tone.

Pathways

TXB2 is part of the arachidonic acid pathway and is linked to the regulation of hemostasis. This pathway interacts with prostacyclin forming a balance between constriction and dilation of blood vessels. TXB2 emerges from the cyclooxygenase (COX) enzyme’s activity closely connected with other pathway members like prostaglandins and leukotrienes. It provides a measurement for understanding Thromboxane A2’s effects and the body's response to injury or inflammation.

TXB2 relates to cardiovascular conditions including myocardial infarction and stroke. Its involvement in platelet aggregation can contribute to the development of these conditions. TXB2 levels can serve as an indicator of imbalance in hemostatic functions. The target's connection with proteins such as COX-1 and COX-2 underlies its role in pathological processes involving thrombotic diseases. Monitoring TXB2 can help evaluate disease risk and response to anti-inflammatory or anticoagulant therapies.
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Product protocols

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Target data

See full target information TXB2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Diabetes & vascular disease research 19:14791641221131788 PubMed36357361

2022

Monitoring and recovery of hyperglycaemia-induced endothelial dysfunction with rheopheresis in diabetic lower extremity ulceration with hyperviscosity.

Applications

Unspecified application

Species

Unspecified reactive species

Kristóf Gál,Georgina Asbóth,Melinda Vass,Attila Bíró,Arnold Markovich,Judit Homoki,Gábor Fidler,Melinda Paholcsek,Zoltán Cziáky,Norbert Németh,Judit Remenyik,Pál Soltész

Pharmaceutical biology 60:175-184 PubMed35014931

2022

The antithrombosis effect of dehydroandrographolide succinate: and studies.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Yin,Shuhua Zhang,Yuxi Huang,Yuanzhu Long,Yiguo Chen,Shiyun Zhao,Aiqun Zhou,Minghua Cao,Xiaoming Yin,Daya Luo

Journal of thrombosis and haemostasis : JTH 20:705-715 PubMed34784445

2021

Variation of platelet function in clinical phenotypes of acute venous thromboembolism - Results from the GMP-VTE project.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Panova-Noeva,Bianca Wagner,Markus Nagler,Thomas Koeck,Vincent Ten Cate,Lisa Eggebrecht,Jürgen H Prochaska,Imke Meyer,Christoph Gerdes,Henri M Spronk,Karl J Lackner,Hugo Ten Cate,Kirsten Leineweber,Stefan Heitmeier,Stavros Konstantinides,Philipp S Wild

EBioMedicine 60:102978 PubMed32920367

2020

Comprehensive platelet phenotyping supports the role of platelets in the pathogenesis of acute venous thromboembolism - results from clinical observation studies.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Panova-Noeva,Bianca Wagner,Markus Nagler,Thomas Koeck,Vincent Ten Cate,Jürgen H Prochaska,Stefan Heitmeier,Imke Meyer,Christoph Gerdes,Volker Laux,Stavros Konstantinides,Henri M Spronk,Thomas Münzel,Karl J Lackner,Kirsten Leineweber,Hugo Ten Cate,Philipp S Wild

Journal of ethnopharmacology 262:113151 PubMed32736050

2020

Is it safe to take Radix Salvia Miltiorrhiza - Radix Pueraria Lobate product with warfarin and aspirin? A pilot study in healthy human subjects.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Zhang,Mengbi Yang,Nicolas James Ho,Rosina Yau Mok,Zhen Zhang,Beikang Ge,Ping Chung Leung,Kwok Pui Fung,Clara Bik San Lau,Vincent Hon Leung Lee,Zhixiu Lin,Raymond Siu Ming Wong,Zhong Zuo

The Journal of physiology 595:6623-6634 PubMed28877347

2017

Sex differences in the role of phospholipase A -dependent arachidonic acid pathway in the perivascular adipose tissue function in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulla A Ahmad,Michael D Randall,Richard E Roberts
View all publications
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