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AB178632

Anti-Thyroid Peroxidase ELISA Kit

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(2 Publications)

Anti-Thyroid Peroxidase ELISA Kit is an indirect ELISA for the qualitative detection of class antibodies against Thyroid Peroxidase ELISA Kit in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation

View Alternative Names

Thyroid peroxidase, TPO

1 Images
Indirect ELISA - Anti-Thyroid Peroxidase ELISA Kit (AB178632)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Thyroid Peroxidase ELISA Kit (AB178632)

Representative standard curve using ab178632

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Sensitivity

< 0.5 U/mL

Range

5 - 320 U/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Thyroid Peroxidase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to thyroid peroxidase in Human serum or plasma (citrate).

A 96-well plate has been precoated with recombinant thyroid peroxidase to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized thyroid peroxidase-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of thyroid peroxidase sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Negative Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178632-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Thyroid peroxidase Standard C - 10 U/mL", "number":"AB178632-CMP11", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroid peroxidase Standard B - 5 U/mL", "number":"AB178632-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroid peroxidase Negative Control", "number":"AB178632-CMP07", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroid peroxidase Standard A - 0 U/mL", "number":"AB178632-CMP09", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroid peroxidase Positive Control", "number":"AB178632-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroid peroxidase Standard E - 80 U/mL", "number":"AB178632-CMP13", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroid peroxidase Standard D - 20 U/mL", "number":"AB178632-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB178632-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Thyroid peroxidase Coated Microplate (12 x 8 wells)", "number":"AB178632-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Thyroid peroxidase Anti-IgG HRP Conjugate", "number":"AB178632-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB178632-CMP16", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB178632-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB178632-CMP03", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB178632-CMP02", "productcode":"" }, { "size":"1 x 2 mL", "name":"Thyroid peroxidase Standard F - 320 U/mL", "number":"AB178632-CMP14", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB178632-CMP15", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anti-Thyroid Peroxidase targets the enzyme Thyroid Peroxidase (TPO) which is essential in the synthesis of thyroid hormones. Also known as thyroperoxidase this protein has a mass of approximately 105 kDa. Thyroid Peroxidase is mainly expressed in the thyroid gland where it catalyzes the iodination of tyrosine residues in thyroglobulin a critical step in the production of the hormones thyroxine (T4) and triiodothyronine (T3).
Biological function summary

Thyroid Peroxidase facilitates the organification of iodine converting iodide ions into reactive iodine intermediates. It operates in conjunction with hydrogen peroxide provided by the enzyme Dual Oxidase (DUOX) forming a vital enzymatic complex within the apical membrane of thyroid follicular cells. This complex is necessary for the proper iodination of tyrosyl residues and subsequent coupling reactions that form T4 and T3 which regulate metabolism and normal growth.

Pathways

Thyroid Peroxidase plays an important role in the thyroid hormone synthesis pathway which is important for metabolic control and regulation of various physiological processes. It is closely linked with Thyroglobulin which serves as the substrate for iodination. Additionally it is part of the pathway that involves the Hypothalamus-Pituitary-Thyroid (HPT) axis maintaining homeostasis of thyroid hormones throughout the body via feedback mechanisms that involve proteins such as Thyrotropin-Releasing Hormone (TRH) and Thyroid-Stimulating Hormone (TSH).

Anti-Thyroid Peroxidase antibodies are markers of autoimmune thyroid diseases such as Hashimoto's thyroiditis and Graves' disease. These conditions cause the immune system to target Thyroid Peroxidase resulting in hypothyroidism or hyperthyroidism respectively. In these disorders the presence of Anti-Thyroglobulin can also be significant as it may indicate a concurrent autoimmune response impacting thyroid function.
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Product protocols

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Target data

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
See full target information Thyroid peroxidase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational autoimmunity 4:100132 PubMed34816112

2021

Multiple autoimmunity and epitope spreading in monozygotic twins.

Applications

Unspecified application

Species

Unspecified reactive species

Esperanza Avalos-Díaz,Elena Pérez-Pérez,Julio Granados,Deyanira Pacheco-Tovar,Juan-José Bollain-Y-Goytia-de-la-Rosa,Rafael Herrera-Esparza

Oncology letters 10:1875-1881 PubMed26622768

2015

/ mutational status in familial non-medullary thyroid carcinomas: A retrospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Landriscina,Maria Iole Natalicchio,Olga Lamacchia,Antonella Conserva,Annamaria Piscazzi,Anna Ciampolillo,Matteo Zingrillo,Antonio Pennella,Pantaleo Bufo,Giulia Vita,Raffaele Antonetti,Eugenio Maiorano,Francesco Giorgino,Mauro Cignarelli
View all publications
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