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AB282910

Tocilizumab ELISA Kit

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(1 Publication)

Tocilizumab ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Tocilizumab in Human in Biofluids samples.
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Sandwich ELISA - Tocilizumab ELISA Kit (AB282910)
  • sELISA

Supplier Data

Sandwich ELISA - Tocilizumab ELISA Kit (AB282910)

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 10 ng/mL

Range

10 - 300 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Note: Tocilizumab ELISA kit ab282910 is intended for Research Use Only, not for use in diagnostic procedures

Tocilizumab ELISA kit is a sandwich ELISA assay for the quantification of Tocilizumab concentration in human serum or plasma with high sensitivity and reproducibility. Standards and samples (serum or plasma) are added in the microtiter plate coated with the reactant for Tocilizumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to Tocilizumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Tocilizumab in the sample or standard. Results of samples can be determined directly using the standard curve.

This assay measures only free Tocilizumab.

Cross Reactivity: Except for Tocilizumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins.

This product is manufactured by BioVision, an Abcam company and was previously called E4858 BioSim™ Tocilizumab (Human) ELISA Kit. E4858-100 is the same size as the 1 x 96 tests size of ab282910.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 30" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 30" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282910-1x96Tests", "assetComponentDetails": [ { "size":"1 x 300 µL", "name":"Tocilizumab Standard S6", "number":"AB282910-CMP13", "productcode":"" }, { "size":"1 x 300 µL", "name":"Tocilizumab Standard S5", "number":"AB282910-CMP12", "productcode":"" }, { "size":"1 x 300 µL", "name":"Tocilizumab Standard S1", "number":"AB282910-CMP08", "productcode":"" }, { "size":"1 x 300 µL", "name":"Tocilizumab Standard S2", "number":"AB282910-CMP09", "productcode":"" }, { "size":"1 x 300 µL", "name":"Tocilizumab Standard S3", "number":"AB282910-CMP10", "productcode":"" }, { "size":"1 x 300 µL", "name":"Tocilizumab Standard S4", "number":"AB282910-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Wash Buffer (20X)", "number":"AB282910-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB substrate", "number":"AB282910-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282910-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plate sealers", "number":"AB282910-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP-conjugate Probe", "number":"AB282910-CMP02", "productcode":"" }, { "size":"2 x 50 mL", "name":"Assay Buffer", "number":"AB282910-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Reactant for Tocilizumab coated microplate", "number":"AB282910-CMP04", "productcode":"" }, { "size":"1 x 300 µL", "name":"Tocilizumab Standard S7", "number":"AB282910-CMP14", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Tocilizumab
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pediatric rheumatology online journal 21:99 PubMed37700264

2023

Subcutaneous tocilizumab in the management of non-infectious uveitis in children: a brief report.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Burlo,Cristina Tumminelli,Serena Pastore,Gabriele Stocco,Debora Curci,Marianna Lucafò,Alberto Tommasini,Andrea Taddio
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com