Anti-Tocilizumab ELISA Kit

Note: Anti-Tocilizumab ELISA kit ab282911 is intended for Research Use Only, not for use in diagnostic procedures

Anti-Tocilizumab ELISA kit is a sandwich ELISA assay for the qualitative detection of antibodies against Tocilizumab with high specificity and sensitivity in serum and plasma samples. Controls and samples are incubated in the microtiter plate coated with the drug Tocilizumab. After incubation, the wells are washed. Then, HRP conjugated probe is added and binds to Tocilizumab antibodies captured by the drug Tocilizumab on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Tocilizumab antibodies in the sample or controls. The results can be evaluated with using cut-off value.

Cross Reactivity: Tocilizumab infusion camouflages/masks the presence of antibody to Tocilizumab in serum/plasma samples. Therefore, blood sampling time is critical for detection of anti-drug-antibodies. It is convenient to obtain blood sample just before the infusion or at least 2 weeks after the infusion of Tocilizumab.

This product is manufactured by BioVision, an Abcam company and was previously called E4859 BioSim™ anti-Tocilizumab (Human) ELISA Kit. E4859-100 is the same size as the 1 x 96 tests size of ab282911.