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Total Aflatoxin ELISA Kit is a Competitive ELISA kit for the measurement of Total Aflatoxin.

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Images

Competitive ELISA - Total Aflatoxin ELISA Kit (AB285282), expandable thumbnail

Key facts

Detection method
Colorimetric
Assay type
Competitive
Sensitivity
= 0.02 ng/mL

Reactivity data

Application
cELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

What's included?

96 Test
Components
20X Wash buffer
1 x 40 mL
Antibody Working Solution
1 x 5.5 mL
ELISA Microplate
1 x 1 Unit
HRP Conjugate
1 x 5.5 mL
Plate Sealer
3 x 1 Unit
Standard S1
1 x 1 mL
Standard S2
1 x 1 mL
Standard S3
1 x 1 mL
Standard S4
1 x 1 mL
Standard S5
1 x 1 mL
Standard S6
1 x 1 mL
Stop Solution
1 x 6 mL
Substrate Reagent A
1 x 6 mL
Substrate Reagent B
1 x 6 mL

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Total Aflatoxin ELISA Kit is a Competitive ELISA kit for the measurement of Total Aflatoxin.

Key facts

Detection method
Colorimetric
Assay type
Competitive
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.02 ng/mL

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

This Total Aflatoxin ELISA Kit (ab285282, E4747) is based on the Competitive ELISA method. It can detect Aflatoxin (AF) in samples, such as grain, peanut, formula feed, etc.

The microplate provided in this kit has been pre-coated with coupled antigen. During the reaction, AF in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AF antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development.

There is a negative correlation between the OD value of samples and the concentration of AF. The concentration of AF in the samples can be calculated by comparing the OD of the samples to the standard curve.

This product is manufactured by BioVision, an Abcam company and was previously called E4747 Total Aflatoxin ELISA Kit. E4747-100 is the same size as the 96 test size of ab285282.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Competitive ELISA - Total Aflatoxin ELISA Kit (ab285282), expandable thumbnail

    Competitive ELISA - Total Aflatoxin ELISA Kit (ab285282)

    Example of standard curve using ab285282. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com