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AB108685

Triiodothyronine (T3) ELISA Kit

4

(1 Review)

|

(4 Publications)

Triiodothyronine (T3) ELISA Kit is a competitive ELISA designed to quantify Triiodothyronine (T3) with a sensitivity of 0.05 ng/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
1 Images
Competitive ELISA - Triiodothyronine (T3) ELISA Kit (AB108685)
  • cELISA

Unknown

Competitive ELISA - Triiodothyronine (T3) ELISA Kit (AB108685)

Representative Standard Curve using ab108685

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 0.05 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.

A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 9.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 40, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108685-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"50X Wash Solution", "number":"AB108685-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108685-CMP13", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108685-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"T3 Standard 4 - 5.0 ng/mL", "number":"AB108685-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-T3 IgG Coated Microplate (12 x 8 wells)", "number":"AB108685-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108685-CMP14", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foils", "number":"AB108685-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"T3 Standard 3 - 2.5 ng/mL", "number":"AB108685-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"T3 Standard 0 - 0.0 ng/mL", "number":"AB108685-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"T3 Standard 2 - 1.0 ng/mL", "number":"AB108685-CMP08", "productcode":"" }, { "size":"1 x 12.5 mL", "name":"Conjugate Buffer", "number":"AB108685-CMP04", "productcode":"" }, { "size":"1 x 1.4 mL", "name":"11X T3-HRP Conjugate", "number":"AB108685-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"T3 Standard 5 - 7.5 ng/mL", "number":"AB108685-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"T3 Standard 1 - 0.5 ng/mL", "number":"AB108685-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Triiodothyronine (T3) also known as liothyronine is a thyroid hormone with a molecular mass of approximately 651.97 Da. It forms when enzymes called deiodinases especially in the liver and kidneys remove an iodine from thyroxine (T4). T3 is mainly expressed in cells of tissues with high metabolic activity including the heart liver and brain. In plasma T3 travels bound to proteins like thyroxine-binding globulin which helps maintain its stable concentration.
Biological function summary

T3 plays a central role in regulating metabolic processes and energy homeostasis. It acts on nearly every cell in the body influencing protein synthesis increasing basal metabolic rate and consequently affecting development growth and metabolism. T3 exerts its effects by binding to nuclear thyroid hormone receptors forming a hormone-receptor complex that regulates gene expression. This hormone engages in crosstalk with other signaling molecules linking it to broader metabolic networks.

Pathways

T3 integrates into the thyroid hormone signaling pathway influencing various metabolic and physiological functions. This pathway includes the conversion of T4 to T3 by iodothyronine deiodinases. T3 also interacts with the PI3K/Akt pathway an important signaling cascade involved in cellular survival and growth. Proteins like thyroid receptor alpha (TRα) and beta (TRβ) are vital in mediating the effects of T3 as they serve as binding sites for the hormone influencing the pathway's downstream function.

Alterations in T3 levels associate with conditions such as hyperthyroidism and hypothyroidism. Hyperthyroidism results from excess T3 leading to symptoms like increased heart rate and weight loss while hypothyroidism stems from T3 deficiency causing fatigue and weight gain. T3 plays a role in Graves’ disease an autoimmune disorder that can cause hyperthyroidism with thyroid-stimulating hormone receptor antibodies influencing the overproduction of the hormone. Dysregulation of T3 levels also impacts the cardiovascular system through its connection with proteins involved in heart function potentially contributing to heart disease.
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Product protocols

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Target data

See full target information Liothyronine
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:406 PubMed35468877

2022

Ageing related thyroid deficiency increases brain-targeted transport of liver-derived ApoE4-laden exosomes leading to cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Manman Zhang,Wenliang Gong,Dianjun Zhang,Ming Ji,Binjie Chen,Beina Chen,Xinyu Li,Yuefei Zhou,Chengyi Dong,Gehua Wen,Xiaoni Zhan,Xiafang Wu,Lulu Cui,Yuliang Feng,Siman Wang,Huiya Yuan,Enyu Xu,Maosheng Xia,Alexei Verkhratsky,Baoman Li

Human cell 33:545-558 PubMed32146707

2020

Thyroid-related hormones as potential markers of hypoxia/ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Naoto Tani,Mayumi Ishikawa,Miho Watanabe,Tomoya Ikeda,Takaki Ishikawa

PloS one 13:e0190987 PubMed29320567

2018

Myocardium of patients with dilated cardiomyopathy presents altered expression of genes involved in thyroid hormone biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Gil-Cayuela,Ana Ortega,Estefanía Tarazón,Luis Martínez-Dolz,Juan Cinca,José Ramón González-Juanatey,Francisca Lago,Esther Roselló-Lletí,Miguel Rivera,Manuel Portolés

Journal of neuroendocrinology 29: PubMed28199771

2017

Thyroid dysfunction in children with autism spectrum disorder is associated with folate receptor α autoimmune disorder.

Applications

Unspecified application

Species

Unspecified reactive species

R E Frye,R Wynne,S Rose,J Slattery,L Delhey,M Tippett,S G Kahler,S C Bennuri,S Melnyk,J M Sequeira,E V Quadros
View all publications
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websiteProtocolBooklet
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