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AB133032

Urinary Prostacyclin ELISA kit

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(1 Publication)

Urinary Prostacyclin ELISA kit is a Competitive ELISA for the measurement of Urinary Prostacyclin in Human in Biofluids, Cell Culture Media samples.
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Competitive ELISA - Urinary Prostacyclin ELISA kit (AB133032)
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Supplier Data

Competitive ELISA - Urinary Prostacyclin ELISA kit (AB133032)

Representative Standard Curve using ab133032

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture supernatant, Tissue Culture Media

Reacts with

Human

Assay type

Competitive

Sensitivity

= 6.58 pg/mL

Range

7.81 - 2000 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human Urinary Prostacyclin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostacyclin breakdown products in urine. The kit measures the two major breakdown products of Prostacyclin in urine, 6-keto-Prostaglandin F1 alpha (6k-PGF1 alpha) and 2,3-dinor-6-keto-Prostaglandin F1 alpha (2,3d-6k-PGF1 alpha).

A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Urinary Prostacyclin antigen and a polyclonal sheep antibody specific to Urinary Prostacyclin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Urinary Prostacyclin captured in the plate.

Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6k-PGF1α and 2,3d-6k- PGF1α. 6-keto- PGF1α is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6k- PGF1α.

Cross Reactivity

The cross reactivity for a number of related eicosanoid compounds was determined.

Compound Cross Reactivity
2,3-dinor-6-keto-PGF1α 100%
6-keto-PGF1α 89.14%
PGF1α 13.21%
PGF2α 4.99%
13,14 dihydro-PGF1α 2.55%
15-keto-PGF2α 0.32%
PGE1 0.08%
PGD2 0.03%
6,15-diketo-13,14 dihydro-PGF1α 0.03%
PGB1 0.01%
PGE2 0.01%
PGA2 0.01%
TXB2 0.01%
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Precision

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 8, "mean": "223 pg/mL", "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 8, "mean": "23 pg/mL", "standardDeviation": null, "coefficientOfVariability": "9.6" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 8, "mean": "72 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 24, "mean": "200 pg/mL", "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 24, "mean": "23 pg/mL", "standardDeviation": null, "coefficientOfVariability": "9.1" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 24, "mean": "66 pg/mL", "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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Recovery

[ { "sample": "Tissue Culture Media", "range": null, "average": "= 109.2" }, { "sample": "Urine", "range": null, "average": "= 101.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133032-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB133032-CMP02", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133032-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Donkey anti-sheep IgG Microplate (12 x 8 wells)", "number":"AB133032-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"Urinary Prostacyclin Alkaline Phosphatase Conjugate", "number":"AB133032-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"Urinary Prostacyclin Antibody", "number":"AB133032-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133032-CMP03", "productcode":"" }, { "size":"1 x 500 µL", "name":"Human Urinary Prostacyclin Standard", "number":"AB133032-CMP07", "productcode":"" }, { "size":"1 x 27 mL", "name":"Assay Buffer", "number":"AB133032-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133032-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostacyclin also known as PGI2 is a member of the prostaglandin family and functions primarily as a vasodilator and inhibitor of platelet aggregation. This compound is synthesized by the enzyme prostacyclin synthase which has a molecular weight of approximately 50 kDa. Prostacyclin is expressed in endothelial cells lining the blood vessels and is quickly released upon synthesis to exert its effects on smooth muscle cells and platelets.
Biological function summary

Prostacyclin plays important roles in vascular homeostasis and hemostasis. It works by increasing cyclic adenosine monophosphate (cAMP) levels in the target cells leading to smooth muscle relaxation and reduced platelet activation. It is not a part of a traditional protein complex but its actions are tightly regulated through interactions with its receptor the prostacyclin receptor (IP receptor) on target cells.

Pathways

Prostacyclin is an integral element of the arachidonic acid pathway which is key in inflammation and thrombosis. It is synthesized from prostaglandin H2 (PGH2) by the action of prostacyclin synthase. This pathway also includes other prostaglandins such as thromboxane A2 which counteracts the effects of prostacyclin by promoting platelet aggregation and vasoconstriction. These prostaglandins work in tandem to maintain vascular balance.

Prostacyclin is implicated in cardiovascular diseases particularly in the pathophysiology of pulmonary hypertension where its vasodilatory properties are beneficial. Loss of prostacyclin production is observed in conditions such as atherosclerosis leading to increased risk of thrombosis and vessel constriction. Related proteins including thromboxane A2 are often studied alongside prostacyclin to understand the balance between vasodilation and vasoconstriction in cardiovascular health.
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Product protocols

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Target data

See full target information Prostacyclin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0161334 PubMed27529478

2016

Enhanced Pulmonary Vascular and Alveolar Development via Prenatal Administration of a Slow-Release Synthetic Prostacyclin Agonist in Rat Fetal Lung Hypoplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Umeda,Shigeru Miyagawa,Satsuki Fukushima,Noriko Oda,Atsuhiro Saito,Yoshiki Sakai,Yoshiki Sawa,Hiroomi Okuyama
View all publications
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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