Skip to main content

VILIP1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of VILIP1 in Serum, Cerebral Spinal Fluid samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - VILIP1 ELISA Kit (AB300324), expandable thumbnail
  • Sandwich ELISA - VILIP1 ELISA Kit (AB300324), expandable thumbnail
  • Sandwich ELISA - VILIP1 ELISA Kit (AB300324), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Serum, Cerebral Spinal Fluid

Assay type

Sandwich (quantitative)

Reactive species

Cow, Human

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Sensitivity

= 18.144 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.

Alternative names

What's included?

96 Tests
Components
10X VILIP1 Capture Antibody
1 x 600 µL
10X VILIP1 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 96 Wells
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL
VILIP1 Lyophilized Recombinant Protein
2 x 1 Vial

Recommended products

VILIP1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of VILIP1 in Serum, Cerebral Spinal Fluid samples.

Key facts

Detection method

Colorimetric

Sample types

Serum, Cerebral Spinal Fluid

Assay type

Sandwich (quantitative)

Reactive species

Cow, Human

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 18.144 pg/mL

Precision

Intra assay

Sample

Serum

n
8
mean
-
SD
-
C.V.

6.7

Inter assay

Sample

Serum

n
3
mean
-
SD
-
C.V.

14.7

Recovery

Sample specific recovery

Sample type

Cerebral Spinal Fluid

Average %

= 99

Range

93 - 103 %

Sample type

Serum

Average %

= 82

Range

80 - 83 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Visinin-like protein 1 commonly abbreviated as VILIP1 is a neuronal calcium sensor protein. It has a mass of about 22 kDa and consists of four EF-hand motifs that bind calcium ions. VILIP1 is primarily expressed in the central nervous system including regions such as the hippocampus and cerebral cortex. This protein interacts with membranes and other intracellular molecular targets in response to changes in calcium levels influencing cellular activities associated with calcium signaling.

Biological function summary

Visinin-like protein 1 modulates various physiological processes within neurons. It regulates signal transduction pathways by mediating calcium-dependent interactions with other proteins. VILIP1 may be part of complexes with other signaling proteins that participate in neurotransmitter release and synaptic plasticity. Its ability to modulate the phosphorylation status of its binding partners can influence neuronal excitability and synaptic communication.

Pathways

Various studies highlight how visinin-like protein 1 participates in critical signaling modules. This protein contributes to the regulation of the cAMP signaling pathway where it can affect the activation of adenylate cyclase. Moreover VILIP1 interacts with signaling pathways related to nicotinic acetylcholine receptors affecting the transmission of nerve impulses. It influences the dynamics between calcium signaling and cAMP affecting processes like learning and memory.

Associated diseases and disorders

Visinin-like protein 1 relates significantly to neurodegenerative diseases. Research findings associate elevated VILIP1 levels with Alzheimer's disease where it can serve as a biomarker for disease progression. Furthermore it connects to Parkinson's disease given its role in neuronal calcium homeostasis a critical factor in disease pathology. In Alzheimer's disease VILIP1 levels can correlate with beta-amyloid plaque development and tau protein phosphorylation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - VILIP1 ELISA Kit (ab300324), expandable thumbnail

    Sandwich ELISA - VILIP1 ELISA Kit (ab300324)

    Example of VILIP1 standard curve in Sample Diluent NS.

    Example of VILIP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - VILIP1 ELISA Kit (ab300324), expandable thumbnail

    Sandwich ELISA - VILIP1 ELISA Kit (ab300324)

    Interpolated concentrations of spiked VILIP1 in bovine cerebrospinal fluid (CSF) and human serum.

    Recombinant VILIP1 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows: human serum 25% and bovine CSF 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - VILIP1 ELISA Kit (ab300324), expandable thumbnail

    Sandwich ELISA - VILIP1 ELISA Kit (ab300324)

    Interpolated concentrations of native VILIP1 in bovine and human CSF.

    Interpolated concentrations of native VILIP1 were measured in duplicate in 50% bovine and human CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com