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AB213327

anti-Zika virus IgM ELISA kit (μ-capture)

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(12 Publications)

anti-Zika virus IgM ELISA kit (?-capture) is an indirect ELISA for the qualitative detection of IgM class antibodies against Zika virus in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (qualitative)

Sensitivity

= 100 %

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Zika virus IgM ELISA (Enzyme-Linked Immunosorbent Assay) kit detects IgM antibodies specific to Zika virus in human serum and plasma. ELISA protocols are available in Spanish and English.

Microplates are coated with anti-human IgM-class antibodies to bind the corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP) labelled Zika virus antigen is added. This antigen-conjugate binds to the captured specific IgM antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.

The intensity of this product is proportional to the amount of specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour.

Absorbance at 450/620 nm is read using an ELISA microwell plate reader.

Zika virus (ZIKV) is a single-stranded RNA virus of the Flaviviridae family (genus Flavivirus). It was first isolated in 1947 from a sentinel rhesus monkey during a yellow fever study in the Zika forest of Uganda.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.65" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.59" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.92" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.02" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.7" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.17" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213327-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Zika Virus IgM Negative Control", "number":"AB213327-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"Zika Virus IgM Cut-off Control", "number":"AB213327-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB213327-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"Zika Virus Conjugate", "number":"AB213327-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB213327-CMP01", "productcode":"" }, { "size":"1 x 96 Test", "name":"Zika Virus IgM Microplate", "number":"AB213327-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB213327-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB213327-CMP04", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB213327-CMP03", "productcode":"" }, { "size":"1 x 2 mL", "name":"Zika Virus IgM Positive Control", "number":"AB213327-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IgM also known as immunoglobulin M is a type of antibody with a molecular mass of approximately 970 kDa. It is the largest antibody present in the human circulatory system mostly found in plasma and lymphatic tissue. IgM is secreted primarily by B cells as a pentamer with a J chain providing a high avidity for antigens. When bound to an antigen IgM can activate the complement system a part of the immune system that helps clear pathogens from an organism.
Biological function summary

IgM acts as the first line of defense during the initial stages of an immune response. It plays an important part in agglutination and neutralization of pathogens forming a complex that alerts other components of the immune system to take action. IgM exists before class-switching to IgG providing immediate protection. It also plays a role in antibody-dependent cellular cytotoxicity. Although not typically a long-term solution its presence is critical for providing initial protection until more specific antibodies like IgG and IgA are produced.

Pathways

The immune response that involves IgM begins with the activation of the classical complement pathway. This pathway bridges the innate and adaptive immunity ensuring the communication and coordination of immune responses. The pathway involves C1q protein binding to IgM-antigen complexes. IgM also interacts with antigen-presenting cells facilitating the processing of antigens and interaction with T cells. These interactions connect IgM with other proteins like IgG and IgA influencing various immune response pathways.

IgM is associated with conditions such as Waldenström's macroglobulinemia and rheumatoid arthritis. Waldenström's macroglobulinemia involves the overproduction of IgM by clonal B cells leading to symptoms like anemia bleeding and neuropathy. Rheumatoid arthritis shows increased IgM levels as part of the autoimmune response affecting joints. In these diseases the IgM antibody interacts with rheumatoid factor and other immune proteins involved in inflammation demonstrating its significant role in autoimmunity and abnormal immune responses.
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Product protocols

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Target data

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of medical virology 97:e70384 PubMed40400258

2025

Seroprevalence and Placental Transfer of Zika and Dengue Virus Antibodies in Postpartum Women in Southeast Brazil.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Rahal Guaragna Machado,Marcella Sanches Peres,Samuel Santos Pereira,Ralyria Mello,Danielle Bastos Araujo,Camila Soares Pereira,Boris Pastorino,Danielle Bruna Leal Oliveira,Michele da Silva Jordan Faleiros,Rossana Pulcineli Vieira Franscisco,Carlos Tadashi Yoshizaki,Silvia Maria Ibidi,Xavier de Lamballerie,Luís Carlos de Sousa Ferreira,Edison Luiz Durigon

PLoS pathogens 19:e1011282 PubMed36976812

2023

Frequent first-trimester pregnancy loss in rhesus macaques infected with African-lineage Zika virus.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna R Rosinski,Lauren E Raasch,Patrick Barros Tiburcio,Meghan E Breitbach,Phoenix M Shepherd,Keisuke Yamamoto,Elaina Razo,Nicholas P Krabbe,Mason I Bliss,Alexander D Richardson,Morgan A Einwalter,Andrea M Weiler,Emily L Sneed,Kerri B Fuchs,Xiankun Zeng,Kevin K Noguchi,Terry K Morgan,Alexandra J Alberts,Kathleen M Antony,Sabrina Kabakov,Karla K Ausderau,Ellie K Bohm,Julia C Pritchard,Rachel V Spanton,James N Ver Hoove,Charlene B Y Kim,T Michael Nork,Alex W Katz,Carol A Rasmussen,Amy Hartman,Andres Mejia,Puja Basu,Heather A Simmons,Jens C Eickhoff,Thomas C Friedrich,Matthew T Aliota,Emma L Mohr,Dawn M Dudley,David H O'Connor,Christina M Newman

PLoS pathogens 18:e1010386 PubMed35969617

2022

Early and mid-gestation Zika virus (ZIKV) infection in the olive baboon (Papio anubis) leads to fetal CNS pathology by term gestation.

Applications

Unspecified application

Species

Unspecified reactive species

Sunam Gurung,Darlene Reuter,Abby Norris,Molly Dubois,Marta Maxted,Krista Singleton,Marisol Castillo-Castrejon,James F Papin,Dean A Myers

PLoS neglected tropical diseases 16:e0010623 PubMed35926066

2022

Fetal loss in pregnant rhesus macaques infected with high-dose African-lineage Zika virus.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren E Raasch,Keisuke Yamamoto,Christina M Newman,Jenna R Rosinski,Phoenix M Shepherd,Elaina Razo,Chelsea M Crooks,Mason I Bliss,Meghan E Breitbach,Emily L Sneed,Andrea M Weiler,Xiankun Zeng,Kevin K Noguchi,Terry K Morgan,Nicole A Fuhler,Ellie K Bohm,Alexandra J Alberts,Samantha J Havlicek,Sabrina Kabakov,Ann M Mitzey,Kathleen M Antony,Karla K Ausderau,Andres Mejia,Puja Basu,Heather A Simmons,Jens C Eickhoff,Matthew T Aliota,Emma L Mohr,Thomas C Friedrich,Thaddeus G Golos,David H O'Connor,Dawn M Dudley

Frontiers in immunology 12:703887 PubMed34367162

2021

Adapting Rapid Diagnostic Tests to Detect Historical Dengue Virus Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Echegaray,Peter Laing,Samantha Hernandez,Sully Marquez,Amanda Harris,Ian Laing,Adam Chambers,Neil McLennan,Victor A Sugiharto,Hua-Wei Chen,Sandra Vivero Villagran,Abigail Collingwood,Magelda Montoya,Fausto Bustos Carrillo,Mark P Simons,Philip J Cooper,Andrea Lopez,Gabriel Trueba,Joseph Eisenberg,Shuenn-Jue Wu,William Messer,Eva Harris,Josefina Coloma,Leah C Katzelnick

PloS one 15:e0235877 PubMed33091010

2020

Quantitative definition of neurobehavior, vision, hearing and brain volumes in macaques congenitally exposed to Zika virus.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle R Koenig,Elaina Razo,Ann Mitzey,Christina M Newman,Dawn M Dudley,Meghan E Breitbach,Matthew R Semler,Laurel M Stewart,Andrea M Weiler,Sierra Rybarczyk,Kathryn M Bach,Mariel S Mohns,Heather A Simmons,Andres Mejia,Michael Fritsch,Maria Dennis,Leandro B C Teixeira,Michele L Schotzko,T Michael Nork,Carol A Rasmussen,Alex Katz,Veena Nair,Jiancheng Hou,Amy Hartman,James Ver Hoeve,Charlene Kim,Mary L Schneider,Karla Ausderau,Sarah Kohn,Anna S Jaeger,Matthew T Aliota,Jennifer M Hayes,Nancy Schultz-Darken,Jens Eickhoff,Kathleen M Antony,Kevin Noguchi,Xiankun Zeng,Sallie Permar,Vivek Prabhakaran,Saverio Capuano,Thomas C Friedrich,Thaddeus G Golos,David H O'Connor,Emma L Mohr

Journal of virology 94: PubMed32188737

2020

Maternal Zika Virus (ZIKV) Infection following Vaginal Inoculation with ZIKV-Infected Semen in Timed-Pregnant Olive Baboons.

Applications

Unspecified application

Species

Unspecified reactive species

Sunam Gurung,Hugh Nadeau,Marta Maxted,Jamie Peregrine,Darlene Reuter,Abby Norris,Rodney Edwards,Kimberly Hyatt,Krista Singleton,James F Papin,Dean A Myers

Journal of virology 94: PubMed31597777

2019

Zika Virus Infection, Reproductive Organ Targeting, and Semen Transmission in the Male Olive Baboon.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie Peregrine,Sunam Gurung,Mark C Lindgren,Sanam Husain,Michael T Zavy,Dean A Myers,James F Papin

PLoS pathogens 15:e1007507 PubMed30657788

2019

Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon.

Applications

Unspecified application

Species

Unspecified reactive species

Sunam Gurung,Nicole Reuter,Alisha Preno,Jamie Dubaut,Hugh Nadeau,Kimberly Hyatt,Krista Singleton,Ashley Martin,W Tony Parks,James F Papin,Dean A Myers

Journal of virology 92: PubMed29875247

2018

Translational Model of Zika Virus Disease in Baboons.

Applications

Unspecified application

Species

Unspecified reactive species

Sunam Gurung,Alisha N Preno,Jamie P Dubaut,Hugh Nadeau,Kimberly Hyatt,Nicole Reuter,Bharti Nehete,Roman F Wolf,Pramod Nehete,Dirk P Dittmer,Dean A Myers,James F Papin
View all publications
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