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Human IL-10 ELISPOT Set is a ELISPOT Set for the sensitive detection of Human IL-10 production in a single cell suspension.

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Key facts

Sample types

Suspension cells

Assay type

Sandwich (qualitative)

Reactive species

Human

Reactivity data

Application

ELISPOT

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

Alternative names

What's included?

5 x 96 Tests
Components
Biotinylated detection antibody, clone B-T10
1 x 1 Vial
Bovine Serum Albumin
1 x 1 g
Human IL-10 Capture antibody
1 x 0.5 mL
Ready-to-use BCIP/NBT substrate buffer
1 x 50 mL
Streptavidin - Alkaline Phosphatase conjugated
1 x 50 µL

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Human IL-10 ELISPOT Set is a ELISPOT Set for the sensitive detection of Human IL-10 production in a single cell suspension.

Key facts

Sample types

Suspension cells

Assay type

Sandwich (qualitative)

Reactive species

Human

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Abcam's Human IL-10 ELISPOT Set is an in vitro ELISPOT assay designed for the qualitative measurement of IL-10 production and secretion in a single cell suspension.

The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

The ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

Recognizes natural human IL10

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