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AB281964

Human IL-13 ELISpot Kit

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Human IL-13 ELISpot Kit is a ELISPOT kit for the sensitive detection of Human IL-13 production in a single cell suspension.

View Alternative Names

NC30, IL13, Interleukin-13, IL-13

Key facts

Detection method

Colorimetric

Sample types

Suspension cells

Reacts with

Human

Assay type

ELISpot

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISPOT": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-13 ELISpot Kit (ab281964) is a highly specific immunoassay for the quantitative determination of human IL-13 production and secretion from T-cells at a single cell level in conditions, closely comparable to the in-vivo environment with minimal cell manipulation.

A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

For research use only - not intended for diagnostic use.

Please note for 001PC (1 x 96 tests) detection antibody is provided in liquid form, for 005PC (5 x 96 tests) it is provided lyophilized.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "19.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "30.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "15.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" } ]
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab281964-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Biotinylated Human IL-13 Detection antibody (Lyophilised)", "number":"AB281964-CMP02" }, { "size":"5 x 1 Unit", "name":"Human IL-13 Pre-coated 96-well PDVF-bottomed plates", "number":"AB281964-CMP03" }, { "size":"1 x 50 µL", "name":"Streptavidin - Alkaline Phosphatase conjugated", "number":"AB281964-CMP05" }, { "size":"1 x 2 g", "name":"Bovine Serum Albumin", "number":"AB281964-CMP04" }, { "size":"1 x 25 mL", "name":"Ready-to-use BCIP/NBT substrate buffer", "number":"AB281964-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab281964-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human IL-13 Pre-coated 96-well PDVF-bottomed plates", "number":"AB281964-CMP03", "productcode":"" }, { "size":"1 x 2 g", "name":"Bovine Serum Albumin", "number":"AB281964-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Ready-to-use BCIP/NBT substrate buffer", "number":"AB281964-CMP06", "productcode":"" }, { "size":"1 x 10 µL", "name":"Streptavidin - Alkaline Phosphatase conjugated", "number":"AB281964-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated Human IL-13 Detection antibody", "number":"AB281964-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed : 8096327, PubMed : 8097324). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed : 8096327). Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (PubMed : 7903680, PubMed : 8759755). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (By similarity). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Also functions on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils (PubMed : 8639787). Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed : 9013879). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed : 21622864).
See full target information IL13
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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