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AB281966

Human IL-4 ELISpot Kit

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Human IL-4 ELISpot Kit is a ELISPOT kit for the sensitive detection of Human IL-4 production in a single cell suspension.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4

Key facts

Detection method

Colorimetric

Sample types

Suspension cells

Reacts with

Human

Assay type

ELISpot

Assay Platform

Pre-coated microplate (6 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISPOT": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-4 ELISpot Kit (ab281966) is a highly specific immunoassay for the quantitative determination of human IL-4 production and secretion from T-cells at a single cell level in conditions, closely comparable to the in-vivo environment with minimal cell manipulation.

A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

For research use only - not intended for diagnostic use.

Please note for 001PC (1 x 96 tests) detection antibody is provided in liquid form, for 005PC (5 x 96 tests) it is provided lyophilized.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" } ]
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab281966-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Biotinylated Human IL-4 Detection antibody (Lyophilised)", "number":"AB281966-CMP01" }, { "size":"5 x 2 Unit", "name":"Human IL-4 Precoated 96 PVDF-bottomed-well plates", "number":"AB281966-CMP03" }, { "size":"1 x 50 µL", "name":"Streptavidin - Alkaline Phosphatase conjugated", "number":"AB281966-CMP05" }, { "size":"1 x 25 mL", "name":"Ready-to-use BCIP/NBT substrate buffer", "number":"AB281966-CMP06" }, { "size":"1 x 2 g", "name":"Bovine Serum Albumin", "number":"AB281966-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab281966-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 Unit", "name":"Human IL-4 Precoated 96 PVDF-bottomed-well plates", "number":"AB281966-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated Human IL-4 Detection antibody", "number":"AB281966-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"Streptavidin - Alkaline Phosphatase conjugated", "number":"AB281966-CMP05", "productcode":"" }, { "size":"1 x 2 g", "name":"Bovine Serum Albumin", "number":"AB281966-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Ready-to-use BCIP/NBT substrate buffer", "number":"AB281966-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
See full target information IL4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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