Human IL-5 ELISpot Kit is a ELISpot Kit for the sensitive detection of Human IL-5 production in a single cell suspension.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISPOT | Reactivity Reacts | Dilution info - | Notes - |
Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:2653458, PubMed:9010276). Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed:1495999, PubMed:22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed:7613138).
Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5
Human IL-5 ELISpot Kit is a ELISpot Kit for the sensitive detection of Human IL-5 production in a single cell suspension.
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 6 | C.V. 32.4 |
Sample Overall | n 6 | C.V. 11.7 |
Sample Overall | n 6 | C.V. 18.1 |
Sample Overall | n 6 | C.V. 16.5 |
Sample Overall | n 6 | C.V. 37.3 |
Human IL-5 ELISpot Kit (ab281967) is a highly specific immunoassay for the quantitative determination of human IL-5 production and secretion from T-cells at a single cell level in conditions, closely comparable to the in-vivo environment with minimal cell manipulation.
A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.
For research use only - not intended for diagnostic use.
Please note for 001PC (1 x 96 tests) detection antibody is provided in liquid form, for 005PC (5 x 96 tests) it is provided lyophilized.
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