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AB281967

Human IL-5 ELISpot Kit

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Human IL-5 ELISpot Kit is a ELISPOT kit for the sensitive detection of Human IL-5 production in a single cell suspension.

View Alternative Names

Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5

Key facts

Detection method

Colorimetric

Sample types

Suspension cells

Reacts with

Human

Assay type

ELISpot

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISPOT": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-5 ELISpot Kit (ab281967) is a highly specific immunoassay for the quantitative determination of human IL-5 production and secretion from T-cells at a single cell level in conditions, closely comparable to the in-vivo environment with minimal cell manipulation.

A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

For research use only - not intended for diagnostic use.

Please note for 001PC (1 x 96 tests) detection antibody is provided in liquid form, for 005PC (5 x 96 tests) it is provided lyophilized.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "32.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "18.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "16.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "37.3" } ]
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab281967-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Biotinylated Human IL-5 Detection antibody (Lyophilised)", "number":"AB281967-CMP02" }, { "size":"5 x 1 Unit", "name":"Human IL-5 Precoated 96 PVDF-bottomed-well plates", "number":"AB281967-CMP03" }, { "size":"1 x 50 µL", "name":"Streptavidin - Alkaline Phosphatase conjugated", "number":"AB281967-CMP05" }, { "size":"1 x 25 mL", "name":"Ready-to-use BCIP/NBT substrate buffer", "number":"AB281967-CMP06" }, { "size":"1 x 2 g", "name":"Bovine Serum Albumin", "number":"AB281967-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab281967-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Human IL-5 Precoated 96 PVDF-bottomed-well plates", "number":"AB281967-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated Human IL-5 Detection antibody", "number":"AB281967-CMP01", "productcode":"" }, { "size":"1 x 10 µL", "name":"Streptavidin - Alkaline Phosphatase conjugated", "number":"AB281967-CMP05", "productcode":"" }, { "size":"1 x 2 g", "name":"Bovine Serum Albumin", "number":"AB281967-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Ready-to-use BCIP/NBT substrate buffer", "number":"AB281967-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 2653458, PubMed : 9010276). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed : 1495999, PubMed : 22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed : 7613138).
See full target information IL5
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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