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AB281990

Human IL-5 ELISpot Pair

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Human IL-5 ELISpot Pair (ab281990) is part of the assay kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

View Alternative Names

Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5

Key facts

Detection method

Colorimetric

Sample types

Suspension cells

Reacts with

Human

Assay type

ELISpot

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISPOT": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-5 ELISpot Antibody Pair (ab281990) is an antibody pair designed for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level by the ELISpot method.

For the quantitative determination of the frequency of Human IL-5 -producing cells.

For research use only - not intended for diagnostic use.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "18.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "16.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "37.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "32.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.7" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab281990-10x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human IL-5 detection antibody", "number":"AB281990-CMP01", "productcode":"" }, { "size":"2 x 0.5 mL", "name":"Human IL-5 Capture Antibody", "number":"AB281990-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 2653458, PubMed : 9010276). Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed : 1495999, PubMed : 22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed : 7613138).
See full target information IL5
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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