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Human IL-5 ELISpot Pair is a ELISpot Pair for the sensitive detection of Human IL-5 production in a single cell suspension.

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Key facts

Detection method
Colorimetric
Sample types
Suspension cells
Assay type
ELISpot
Reactive species
Human

Reactivity data

Application
ELISPOT
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:2653458, PubMed:9010276). Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed:1495999, PubMed:22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed:7613138).

Alternative names

Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5

What's included?

10 x 96 Tests
Components
Biotinylated Human IL-5 detection antibody
2 x 1 Vial
Human IL-5 Capture Antibody
2 x 0.5 mL

Recommended products

Human IL-5 ELISpot Pair is a ELISpot Pair for the sensitive detection of Human IL-5 production in a single cell suspension.

Key facts

Detection method
Colorimetric
Sample types
Suspension cells
Assay type
ELISpot
Reactive species
Human
Assay Platform
Microplate (12 x 8 well strips)

Precision

Intra assay

Sample
Overall
n
6
C.V.
18.1
Sample
Overall
n
6
C.V.
16.5
Sample
Overall
n
6
C.V.
37.3
Sample
Overall
n
6
C.V.
32.4
Sample
Overall
n
6
C.V.
11.7

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Human IL-5 ELISpot Antibody Pair (ab281990) is an antibody pair designed for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level by the ELISpot method.

For the quantitative determination of the frequency of Human IL-5 -producing cells.

For research use only - not intended for diagnostic use.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com