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Human Interferon gamma + IL-10 ELISPOT Set is a ELISPOT Set for the sensitive detection of Human Interferon gamma + IL-10 production in a single cell suspension.

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Sample types

Suspension cells

Assay type

Sandwich (qualitative)

Reactive species

Human

Reactivity data

Application
ELISPOT
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

What's included?

20 x 96 Tests
Components
10 x concentrate buffer for the preparation of AEC buffer
4 x 5 mL
50 x concentrate AEC substrate buffer
4 x 1 mL
Anti-FITC antibody HRP conjugate
4 x 100 µL
Biotinylated detection antibody, clone B-T10
4 x 1 Vial
Bovine Serum Albumin
4 x 1 g
Human IFN Gamma Capture antibody
4 x 0.5 mL
Human IL-10 Capture antibody
4 x 0.5 mL
IFNγ FITC conjugated detection antibody
4 x 0.55 mL
Ready-to-use BCIP/NBT substrate buffer
4 x 50 mL
Streptavidin - Alkaline Phosphatase conjugated
4 x 50 µL

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Human Interferon gamma + IL-10 ELISPOT Set is a ELISPOT Set for the sensitive detection of Human Interferon gamma + IL-10 production in a single cell suspension.

Key facts

Sample types

Suspension cells

Assay type

Sandwich (qualitative)

Reactive species

Human

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.

The ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

The dual colour ELISPOT allows you to monitor the production of two cytokines simultaneously in the same well.

Principle

After cell stimulation, locally produced cytokines are captured by IFN gamma and IL10 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti-IFN gamma FITC conjugated antibody and a biotinylated anti-IL-10 antibody. Those are in turn recognised by anti-FITC HRP and streptavidin-AP conjugates. PVDF-bottomed-well plates are then incubated first with AEC substrate buffer, washed and subsequently incubated with BCIP/NBT. Coloured red/brownish spots indicate IFN gamma production while IL-10 is revealed by blue/purple spots.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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