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Human TNF alpha ELISPOT Kit (with un-coated plates) is a ELISPOT kit for the sensitive detection of Human TNF alpha production in a single cell suspension.

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Detection method

Colorimetric

Sample types

Suspension cells

Assay type

Sandwich (qualitative)

Reactive species

Human, Macaque monkey

Reactivity data

Application
ELISPOT
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

What's included?

5 x 96 Tests
Components
96 PVDF-bottomed-well plates.
4 x 1 Unit
Bovine Serum Albumin
1 x 1 g
Ready-to-use BCIP/NBT substrate buffer
1 x 50 mL
Streptavidin - Alkaline Phosphatase conjugated
1 x 50 µL
TNFα Biotinylated detection antibody (clone B-C7)
1 x 1 Vial
TNFα Capture antibody (clone B-F7)
1 x 500 µL

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Human TNF alpha ELISPOT Kit (with un-coated plates) is a ELISPOT kit for the sensitive detection of Human TNF alpha production in a single cell suspension.

Key facts

Detection method

Colorimetric

Sample types

Suspension cells

Assay type

Sandwich (qualitative)

Reactive species

Human, Macaque monkey

Assay Platform

Microplate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

The Human TNF alpha ELISPOT Kit (with un-coated plates) is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

This ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Principal:

After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

Recognizes natural human TNF alpha

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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