ELISPOT (Enzyme-Linked ImmunoSpot) is a sensitive immunological assay closely related to the ELISA technique that was developed to detect and quantify secreted proteins, such as cytokines, growth factors, or antibodies, at the single-cell level. ELISPOT assays allow visualization of the secretory product of individually activated or responding cells. Each spot that develops in the assay represents a single reactive cell. They provide valuable insights into the immune response at the single-cell level and are essential in immunology and vaccine research.
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