AB64212

Avidin/Biotin Blocking Kit

Name: Avidin/biotin blocking kit - 15 mL (ab64212) | Abcam
Brand: Abcam Limited
SKU: AB64212
Price: 140 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(39 Publications)

Avidin/biotin blocking kit (ab64212) blocks and minimizes non-specific staining caused by endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection.

- Supplied as ready-to-use reagents- no dilution required
- Cited in over 35 publications

41 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of CD55 staining in a section of formalin-fixed paraffin-embedded human normal lung*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323679 at 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of IFNG staining in a section of formalin-fixed paraffin-embedded human normal tonsil*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322914 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of HLA A staining in a section of formalin-fixed paraffin-embedded human normal small intestine*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322986 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of CD11c staining in a section of formalin-fixed paraffin-embedded human normal tonsil*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322302 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Cytokeratin 8 staining in a section of formalin-fixed paraffin-embedded human normal stomach*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322309 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of THY1 staining in a section of formalin-fixed paraffin-embedded human colon carcinoma*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323685 at 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of APOE staining in a section of formalin-fixed paraffin-embedded human normal liver*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323532 at 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

Low expression staining of SLC2A1 in a section of formalin-fixed paraffin-embedded human normal pancreas*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323677 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Cytokeratin 8 staining in a section of formalin-fixed paraffin-embedded human breast ductal carcinoma.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322309 at 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of CYP3A4 staining in a section of formalin-fixed paraffin-embedded human normal liver*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323530 at 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Tau staining in a section of formalin-fixed paraffin-embedded human normal cerebral cortex*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322984 at 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of KRT14 staining in a section of formalin-fixed paraffin-embedded human normal skin*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323529 at 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of TRBC1 staining in a section of formalin-fixed paraffin-embedded human normal tonsil*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323531 at 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of INS staining in a section of formalin-fixed paraffin-embedded human normal pancreas*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323678 at 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of CD5 staining in a section of formalin-fixed paraffin-embedded human tonsil*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab318315 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Langerin staining in a section of formalin-fixed paraffin-embedded human normal skin*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322985 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of formalin-fixed paraffin-embedded human breast cancer sections* tested on a Leica BOND^TM.

Sections were pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The sections were then incubated with antibody (ab317077, Rabbit monoclonal to ISG15 at 5ug/ml) or isotype control (ab320073, Rabbit IgG, at 5ug/ml) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX. The background control image is taken from an identical assay without primary antibody or isotype control.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of CD21 staining in a section of formalin-fixed paraffin-embedded human normal tonsil*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322303 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of COX2 / Cyclooxygenase 2 staining in a section of formalin-fixed paraffin-embedded human normal small intestine*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322299 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of CD68 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*.

Performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab317601 at 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of CD11b staining in a section of formalin-fixed paraffin-embedded human normal spleen*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322298 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of DPP4 staining in a section of formalin-fixed paraffin-embedded human normal liver*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab318313 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunocytochemistry/ Immunofluorescence - Avidin/Biotin Blocking Kit (AB64212)

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Immunocytochemistry/ Immunofluorescence - Avidin/Biotin Blocking Kit (AB64212)

IHC image of SLC7A5/LAT1 staining in a section of formalin-fixed paraffin-embedded human lung squamous carcinoma*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab317767 at 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of IgG2 staining in a section of formalin-fixed paraffin-embedded human normal tonsil*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322913 at 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of TIMP1 staining in a section of formalin-fixed paraffin-embedded human prostate carcinoma*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab317768 at 5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Bcl6 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab317765 at 10 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

Negative staining of TIMP1 in a section of formalin-fixed paraffin-embedded human normal brain*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab317768 at 5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of SLC2A1 staining in a section of formalin-fixed paraffin-embedded human normal liver*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323677 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Myeloperoxidase staining in a section of formalin-fixed paraffin-embedded human normal spleen*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322301 at 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of C3/C3b staining in a section of formalin-fixed paraffin-embedded mouse testis.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with a322300 at 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Tau staining in a section of formalin-fixed paraffin-embedded mouse normal brain.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322982 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of C3/C3b staining in a section of formalin-fixed paraffin-embedded rat testis.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab322300 at 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Lipocalin-2 staining in a section of formalin-fixed paraffin-embedded mouse spleen.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab318314 at 2μg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of DPP4 staining in a section of formalin-fixed paraffin-embedded normal rat liver.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab317766 at 0.5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Uteroglobin staining in a section of formalin-fixed paraffin-embedded mouse lung.

Performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab321782 at 5μg/ml (1/100), for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of Uteroglobin staining in a section of formalin-fixed paraffin-embedded mouse lung.

Performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab321781 at 0.1 μg/ml (1/5000), for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

Negative staining of KRT14 in a section of formalin-fixed paraffin-embedded human normal liver*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323529 at 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

Micrographs showing T-cells infiltrationMulti-spectral, multicolor Immunofluorescence for T-cells infiltration in lymph nodes samples : dMMR tumor. (200x magnification). Multiplex sequential IF staining on 3-μm sections from FFPE tissue; Tissue sections were treated with an avidin/biotin blocking kit (ab64212) before using a biotin conjugated Foxp3 antibody. Other antibodies were conjugated to fluorochroms. CD4 : red, CD8 : green, CD4/Foxp3 : tile, CD8 blue, EPCAM : yellow

Immunohistochemistry (Frozen sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Avidin/Biotin Blocking Kit (AB64212)

Immunohistochemical analysis staining HTRA1 in mouse bone tissue sections. Tissue sections were dewaxed, rehydrated and treated with Endogenous Avidin/Biotin Blocking Kit (ab64212), 3% H₂O₂ and normal swine serum. Tissue sections were then incubated with a polyclonal anti-HTRA1 antibody for 1 hour at 37°C. After PBS wash, tissue sections are incubated with biotinylated swine anti-rabbit IgG for 45 minutes at 37°C and further incubated for 30 minutes after washing. With Vectastatin. Sections were developed using DAB and counterstained using Harris modified hematoxylin.

Image from Filliat G., PLoS One 12(7). Fig 6a & b. doi: 10.1371/journal.pone.0181600. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

Negative staining of RFP in a section of formalin-fixed paraffin-embedded HEK293T cells transfected with an empty vector containing a his tag.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323934 at 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Avidin/Biotin Blocking Kit (AB64212)

IHC image of RFP staining in a section of formalin-fixed paraffin-embedded HEK293T cells transfected with a Red fluorescent protein expression vector containing a his tag.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323934 at 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Product details

Avidin Biotin Blocking Kit ab64212 blocks signal from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection (eg. with ABC IHC detection kits).

When using the kit, firstly an excess of avidin is added to the sample to bind endogenous biotin, that avidin is then blocked with an excess of biotin. Excess biotin and avidin is washed away.

The kit is often used with cells and tissues containing high levels of biotin. This can be indicated by blocking sections with hydrogen peroxide, and then incubating sections with streptavidin-HRP and then DAB; brown DAB staining indicates endogenous biotin. Kidney, liver, spleen especially contain high levels of biotin.

This kit was previously called Endogenous Avidin/Biotin Blocking Kit.

IHC protocol suitable for use with Avidin Biotin Blocking Kit ab64212:
For frozen sections, skip steps 1 and 2.

1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.

3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, block for endogenous biotin by incubating with avidin block for 15 mins, washing twice, incubating with biotin block for 15 mins, and washing twice.

4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.

5. Apply primary antibody in antibody diluent and incubate.

6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.

7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.

8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC Substrate and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.

9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.

10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.

Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

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What's included?

{ "values": { "15mL": { "sellingSize": "15 mL", "publicAssetCode":"ab64212-15mL", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"Avidin Block", "number":"AB64212-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Biotin Block", "number":"AB64212-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:2061 PubMed38267530

2024

Dose-dependent reduction of somatic expansions but not Htt aggregates by di-valent siRNA-mediated silencing of MSH3 in HdhQ111 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rachelle Driscoll,Lucas Hampton,Neeta A Abraham,J Douglas Larigan,Nadine F Joseph,Juan C Hernandez-Vega,Sarah Geisler,Fu-Chia Yang,Matthew Deninger,David T Tran,Natasha Khatri,Bruno M D C Godinho,Garth A Kinberger,Daniel R Montagna,Warren D Hirst,Catherine L Guardado,Kelly E Glajch,H Moore Arnold,Corrie L Gallant-Behm,Andreas Weihofen

Scientific reports 13:19183 PubMed37932336

2023

Low oral dose of 4-methylumbelliferone reduces glial scar but is insufficient to induce functional recovery after spinal cord injury.

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Species

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Kateřina Štepánková,Milada Chudíčková,Zuzana Šimková,Noelia Martinez-Varea,Šárka Kubinová,Lucia Machová Urdzíková,Pavla Jendelová,Jessica C F Kwok

Frontiers in medicine 10:1191205 PubMed37706027

2023

Laser microdissection, proteomics, and multiplex immunohistochemistry: a bumpy ride into the study of paraffin-embedded fetal and pediatric lung tissues.

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Species

Unspecified reactive species

Luis M Cardoso Dos Santos,Yannick Avila,Domitille Schvartz,Anne-Laure Rougemont,Marie-Luce Bochaton-Piallat,Isabelle Ruchonnet-Metrailler

Veterinary medicine and science 9:1426-1437 PubMed36920334

2023

Evaluation of the in vitro and in vivo effect of liposomal doxorubicin along with oncolytic Newcastle disease virus on 4T1 cell line: Animal preclinical research.

Applications

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Species

Unspecified reactive species

Pooya Faranoush,Alireza Jahandideh,Reza Nekouian,Pejman Mortazavi

International journal of molecular sciences 24: PubMed36835210

2023

4-Methylumbeliferone Treatment at a Dose of 1.2 g/kg/Day Is Safe for Long-Term Usage in Rats.

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Species

Unspecified reactive species

Kateřina Štěpánková,Dana Mareková,Kristýna Kubášová,Radek Sedláček,Karolína Turnovcová,Irena Vacková,Šárka Kubinová,Pavol Makovický,Michaela Petrovičová,Jessica C F Kwok,Pavla Jendelová,Lucia Machová Urdzíková

Experimental and therapeutic medicine 25:132 PubMed36845951

2023

Sclerosing extramedullary hematopoietic tumor of the colon: A case report and literature review.

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Species

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Zhibo Zuo,Qi Zhang,Wanxin Wu,Xin Li,Lin Zhang,Jingyu Wang,Zhiqin Guo,Shaoqing Hu,Hongyan Liu

Scientific reports 12:21825 PubMed36528753

2022

Periportal steatosis in mice affects distinct parameters of pericentral drug metabolism.

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Species

Unspecified reactive species

Mohamed Albadry,Sebastian Höpfl,Nadia Ehteshamzad,Matthias König,Michael Böttcher,Jasna Neumann,Amelie Lupp,Olaf Dirsch,Nicole Radde,Bruno Christ,Madlen Christ,Lars Ole Schwen,Hendrik Laue,Robert Klopfleisch,Uta Dahmen

Nature communications 13:6237 PubMed36284108

2022

Cell-specific bioorthogonal tagging of glycoproteins.

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Species

Unspecified reactive species

Anna Cioce,Beatriz Calle,Tatiana Rizou,Sarah C Lowery,Victoria L Bridgeman,Keira E Mahoney,Andrea Marchesi,Ganka Bineva-Todd,Helen Flynn,Zhen Li,Omur Y Tastan,Chloe Roustan,Pablo Soro-Barrio,Mahmoud-Reza Rafiee,Acely Garza-Garcia,Aristotelis Antonopoulos,Thomas M Wood,Tessa Keenan,Peter Both,Kun Huang,Fabio Parmeggian,Ambrosius P Snijders,Mark Skehel,Svend Kjær,Martin A Fascione,Carolyn R Bertozzi,Stuart M Haslam,Sabine L Flitsch,Stacy A Malaker,Ilaria Malanchi,Benjamin Schumann

Experimental and therapeutic medicine 24:631 PubMed36160893

2022

Mediastinal cavernous angioleiomyoma: A case report and review of literature.

Applications

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Species

Unspecified reactive species

Zhibo Zuo,Wanxin Wu,Xin Li,Lin Zhang,Jingyu Wang,Zhiqin Guo,Shaoqing Hu,Qi Zhang

Liver international : official journal of the International Association for the Study of the Liver 43:208-220 PubMed35924447

2022

DKK1 drives immune suppressive phenotypes in intrahepatic cholangiocarcinoma and can be targeted with anti-DKK1 therapeutic DKN-01.

Applications

Unspecified application

Species

Unspecified reactive species

Edward J Jarman,Marta Horcas-Lopez,Scott H Waddell,Stephanie MacMaster,Konstantinos Gournopanos,Daniel Y H Soong,Kamila I Musialik,Panagiota Tsokkou,Minn-E Ng,William A Cambridge,David H Wilson,Michael H Kagey,Walter Newman,Jeffrey W Pollard,Luke Boulter

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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