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AB183275

DoubleStain Co-Localization IHC Kit: M&M on rodent tissue (Green/HRP & AP/Red)

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DoubleStain IHC Kit: M&M on rodent tissue (Green/HRP & AP/Red)( ab183275) is designed to be used with supplied 2x mouse primary antibodies on mouse or rat tissue or cell samples. Suitable with formalin-fixed, paraffin-embedded (FFPE) or frozen tissue.

- Contains a HRP Polymer anti-Mouse IgG, AP Polymer anti-Mouse IgG and two distinct chromogens: Permanent Red (Red) and Emerald Chromogen (Green)
- Designed to prevent non-specific binding of endogenous biotin
- Non-Aqueous Mounting Medium provided
- Available in different sizes to fit your experimental needs

Key facts

Assay type

Polymer Multiplex

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Doublestain IHC Kit (ab183275) is designed to use with two user supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples. The advantage of this kit series is that it will allow you to visualize when two proteins are co localized by producing a third color blue purple. Specimens can be frozen or paraffin embedded, or freshly prepared monolayer cell smears.

Double staining is a common method used in immunohistochemistry that allows for detection of two distinct antigens in a single tissue. This kit uses an HRP or AP polymer based technology combined with a proprietary blocking buffer system that achieves ultra sensitivity with no background or cross reactivity. Abcam's Doublestain IHC Kit (ab183275) supplies the user with primer system to enhance the two polymer enzyme conjugates; Mouse HRP Polymer and Mouse AP Polymer with two distinct substrates/chromogens, Permanent Red and Emerald. Permanent Red reacts with Mouse AP Polymer conjugate to produce a red color. Emerald chromogen reacts with Mouse HRP Polymer conjugate to produce a green color. However when the chromogens are produced in the same place the color appears blue to purple in color. Abcam's Doublestain IHC Kit (ab183275) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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