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AB183280

DoubleStain IHC Kit: G&Rt on human/mouse tissue (DAB & AP/Red)

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DoubleStain IHC kit: G&Rt on human/mouse tissue (DAB & AP/Red)(ab183280) is designed to be used with supplied goat and rat primary antibodies on human or mouse tissue or cell samples. Suitable with formalin-fixed, paraffin-embedded (FFPE) or frozen tissue.

- Contains AP-Polymer anti-Rat IgG, HRP-Polymer anti-Goat IgG and two distinct chromogens: Permanent Red (Red) and DAB (brown)
- Designed to prevent non-specific binding of endogenous biotin
- Aqueous Mounting Medium provided
- Available in different sizes to fit your experimental needs

Key facts

Assay type

Polymer Multiplex

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DoubleStain IHC kit (ab183280) is designed to use with user supplied goat and rat primary antibodies to detect two distinct antigens on human or mouse tissue or cell samples. . This kit has been tested on paraffin embedded tissue, however it can also be used on frozen specimens or freshly prepared monolayer cell smears.

Double staining is one of most common methods used in immunohistostaining allowing the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183280) supplies the user with two polymer enzyme conjugates: HRP polymer anti-Goat IgG and AP polymer anti-Rat IgG and two distinct substrates/chromogens, DAB (brown) and Permanent Red (red). Users will sequentially apply the two enzyme conjugates onto the specimen. When two proteins are present a brown/red color will develop depending on the presence and location of the antigens the two colors should be distinct. If only the anti-goat antigen is present only the DAB brown chromogen will be seen and if only the Rat antigen is present only the Permanent Red chromogen will be visible. The Kit is a non-biotin system avoiding endogenous biotin non-specific binding.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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