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AB183273

DoubleStain IHC Kit: M&M on rodent tissue (DAB & AP/Red)

5

(1 Review)

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(1 Publication)

DoubleStain IHC Kit: M&M on rodent tissue (DAB & AP/Red) (ab183273) is designed to be used with supplied mouse primary antibodies on mouse and rat tissue or cell samples. Suitable with formalin-fixed, paraffin-embedded (FFPE) or frozen tissue.

- Contains a HRP Polymer anti-Mouse IgG, an AP-Polymer anti-Mouse IgG and two distinct chromogens: DAB (Brown) and Permanent Red (Red)
- Designed to prevent non-specific binding of endogenous biotin
- Aqueous Mounting Medium provided
- Available in different sizes to fit your experimental needs

Key facts

Sample types

Tissue

Assay type

Polymer Multiplex

Reacts with

Mouse, Rat

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Doublestain IHC Kit (ab183273) is designed to use with two user supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples. Specimens can be frozen or paraffin embedded, or freshly prepared monolayer cell smears.

Double staining is a common method used in immunohistochemistry that allows for detection of two distinct antigens in a single tissue. This kit uses an HRP or AP polymer based technology combined with a proprietary blocking buffer system that achieves ultra sensitivity with no background or cross reactivity. Abcam's Doublestain IHC Kit supplies the user with primer system to enhance the two polymer enzyme conjugates Mouse HRP Polymer and Mouse AP Polymer with two distinct substrates/chromogens, Permanent Red and DAB. Permanent Red reacts with Mouse AP Polymer conjugate to produce a red color. DAB chromogen reacts with Mouse HRP Polymer conjugate to produce a brown color. Abcam's Doublestain IHC Kit is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 31:886-903.e8 PubMed38733994

2024

Metabolic regulator ERRγ governs gastric stem cell differentiation into acid-secreting parietal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mahliyah Adkins-Threats,Sumimasa Arimura,Yang-Zhe Huang,Margarita Divenko,Sarah To,Heather Mao,Yongji Zeng,Jenie Y Hwang,Joseph R Burclaff,Shilpa Jain,Jason C Mills
View all publications
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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