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Abcam's Doublestain IHC Kit (ab183273) is designed to use with two user supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples.

Sample type

Tissue

Assay type

Polymer Multiplex

Reactive species

Mouse, Rat

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

What's included?

60 mL
Components
Antibody Blocker (40x)
1 x 100 mL
Aqueous Mounting Medium
1 x 60 mL
Blocker A
1 x 60 mL
Blocker B
1 x 60 mL
DAB Chromogen (20x)
1 x 3.5 mL
DAB Substrate
1 x 120 mL
Mouse AP Polymer
1 x 60 mL
Mouse Antibody Enhancer
1 x 60 mL
Mouse HRP Polymer
1 x 60 mL
Mouse Primer
1 x 60 mL
Permanent Red Activator (5x)
1 x 14 mL
Permanent Red Chromogen (100x)
1 x 700 µL
Permanent Red Substrate
1 x 120 mL

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Abcam's Doublestain IHC Kit (ab183273) is designed to use with two user supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples.

Key facts

Sample type

Tissue

Assay type

Polymer Multiplex

Reactive species

Mouse, Rat

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Abcam's Doublestain IHC Kit (ab183273) is designed to use with two user supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples. Specimens can be frozen or paraffin embedded, or freshly prepared monolayer cell smears.


Double staining is a common method used in immunohistochemistry that allows for detection of two distinct antigens in a single tissue. This kit uses an HRP or AP polymer based technology combined with a proprietary blocking buffer system that achieves ultra sensitivity with no background or cross reactivity. Abcam's Doublestain IHC Kit supplies the user with primer system to enhance the two polymer enzyme conjugates Mouse HRP Polymer and Mouse AP Polymer with two distinct substrates/chromogens, Permanent Red and DAB. Permanent Red reacts with Mouse AP Polymer conjugate to produce a red color. DAB chromogen reacts with Mouse HRP Polymer conjugate to produce a brown color. Abcam's Doublestain IHC Kit is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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