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AB210060

DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC)

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DoubleStain IHC kit: M&R on human tissue (BCIP & AEC)( ab210060) is designed to be used with supplied mouse and rabbit primary antibodies on human tissue or cell samples. Suitable with formalin-fixed, paraffin-embedded (FFPE) or frozen tissue.

- Contains AP-Polymer anti-Rabbit IgG, HRP-Polymer anti-Mouse IgG and two distinct chromogens: BCIP/NBT (Purple) and AEC (Red)
- Designed to prevent non-specific binding of endogenous biotin
- Aqueous Mounting Medium provided
- Available in different sizes to fit your experimental needs

Key facts

Sample types

Tissue

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) (ab210060) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.

Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, and two distinct chromogens. The AEC chromogen (red color) is used with HRP Polymer anti-Mouse IgG and the BCIP/NBT chromogen (purple/blue color) is used with AP Polymer anti-Rabbit IgG. DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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