AB210059

DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red)

Name: DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059) | Abcam
Brand: Abcam Limited
SKU: AB210059
Price: 1995 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(15 Publications)

DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059) is designed to be used with supplied mouse and rabbit primary antibodies on human tissue or cell samples. Suitable with formalin-fixed, paraffin-embedded (FFPE) or frozen tissue.

- Contains a HRP Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG and two distinct chromogens: DAB (Brown) and Permanent Red (Red)
- Designed to prevent non-specific binding of endogenous biotin
- Aqueous Mounting Medium provided
- Available in different sizes to fit your experimental needs

Key facts

Sample types

Tissue

Reacts with

Human

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.

Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, and two distinct chromogens. The DAB chromogen (brown color) is used with the HRP-Polymer anti-Mouse IgG and Permanent Red (red color) is used with the AP-Polymer anti-Rabbit IgG. DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Immunologic research 73:27 PubMed39777574

2025

Inactivation of ATG13 stimulates chronic demyelinating pathologies in muscle-serving nerves and spinal cord.

Applications

Unspecified application

Species

Unspecified reactive species

Molly E Drosen,Sarojini Bulbule,Gunnar Gottschalk,Daniel Peterson,Linda Adrienne Allen,Leggy A Arnold,Avik Roy

American journal of cancer research 14:3083-3103 PubMed39005689

2024

platform to functional precision medicine: clinical relevance in gynecological cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Raed Sulaiman,Jennifer C Aske,Luis Rojas Espaillat,Xiaoqian Lin,David Starks,Adam Dale,Kris Gaster,Pradip De,Nandini Dey

International journal of molecular sciences 25: PubMed38542414

2024

Atlas of Tumor and Tumor Microenvironment Cells of Lymphovascular Space Invasion (LVSI) in High-Grade Serous Endometrial Adenocarcinoma: A Case Study.

Applications

Unspecified application

Species

Unspecified reactive species

Raed Sulaiman,Adam Dale,Xiaoqian Lin,Jennifer C Aske,Kris Gaster,David Starks,Luis Rojas Espaillat,Pradip De,Nandini Dey

International journal of molecular sciences 24: PubMed37446260

2023

Tumor-TME Bipartite Landscape of PD-1/PD-L1 in Endometrial Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Raed Sulaiman,Pradip De,Jennifer C Aske,Xiaoqian Lin,Adam Dale,Nischal Koirala,Kris Gaster,Luis Rojas Espaillat,David Starks,Nandini Dey

PloS one 18:e0284296 PubMed37053156

2023

Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels.

Applications

Unspecified application

Species

Unspecified reactive species

Bruna Stuqui,Paola Jocelan Scarin Provazzi,Maria Leticia Duarte Lima,Ágata Silva Cabral,Ellen Cristina Rivas Leonel,Natalia Maria Candido,Sebastião Roberto Taboga,Márcia Guimarães da Silva,Flávio de Oliveira Lima,Patrícia Pereira Dos Santos Melli,Silvana Maria Quintana,Marilia de Freitas Calmon,Paula Rahal

Frontiers in oncology 12:1051148 PubMed36465358

2022

TGF-β1 promotes human breast cancer angiogenesis and malignant behavior by regulating endothelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Xiong Li,Jie-Xin Chen,Ze-Jun Zheng,Wang-Jing Cai,Xiong-Bin Yang,Yuan-Yuan Huang,Yao Gong,Feng Xu,Yong-Song Chen,Ling Lin

Cancers 14: PubMed35740537

2022

A Laboratory-Friendly CTC Identification: Comparable Double-Immunocytochemistry with Triple-Immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Raed Sulaiman,Pradip De,Jennifer C Aske,Xiaoqian Lin,Adam Dale,Ethan Vaselaar,Nischal Koirala,Cheryl Ageton,Kris Gaster,Joshua Plorde,Benjamin Solomon,Bradley Thaemert,Paul Meyer,Luis Rojas Espaillat,David Starks,Nandini Dey

Pathology oncology research : POR 28:1610230 PubMed35570844

2022

The Diagnostic Significance of CXCL13 in M2 Tumor Immune Microenvironment of Human Astrocytoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Jyuan Chang,Chia-Te Chao,Aij-Lie Kwan,Chee-Yin Chai

Cancer immunology, immunotherapy : CII 71:57-69 PubMed34009409

2021

CD74 macrophages are associated with favorable prognosis and immune contexture in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Xiao,Kangshuai Li,Xiaodong Zhu,Bin Xu,Xuefeng Liu,Ming Lei,Hui-Chuan Sun

PloS one 16:e0246393 PubMed33690604

2021

Linking bacterial enterotoxins and alpha defensin 5 expansion in the Crohn's colitis: A new insight into the etiopathogenetic and differentiation triggers driving colonic inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tanu Rana,Olga Y Korolkova,Girish Rachakonda,Amanda D Williams,Alexander T Hawkins,Samuel D James,Amos M Sakwe,Nian Hui,Li Wang,Chang Yu,Jeffrey S Goodwin,Michael G Izban,Regina S Offodile,Mary K Washington,Billy R Ballard,Duane T Smoot,Xuan-Zheng Shi,Digna S Forbes,Anil Shanker,Amosy E M'Koma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red)

Key facts

Sample types

Reacts with

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (15)

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Product promise