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Abcam's DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies.

Sample type

Tissue

Reactive species

Human

Reactivity data

Application
IHC
Reactivity
Reacts
Dilution info
-
Notes

-

What's included?

18 mL
Components
Aqueous Mounting Medium
1 x 18 mL
DAB Chromogen (20X)
1 x 2 mL
DAB Substrate
1 x 36 mL
Mouse HRP polymer
1 x 18 mL
Permanent Red Activator (5X)
1 x 7.2 mL
Permanent Red Chromogen (100X)
1 x 360 µL
Permanent Red Substrate (RTU)
1 x 36 mL
Rabbit AP polymer
1 x 18 mL

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Abcam's DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies.

Key facts

Sample type

Tissue

Reactive species

Human

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Abcam's DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.

Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, and two distinct chromogens. The DAB chromogen (brown color) is used with the HRP-Polymer anti-Mouse IgG and Permanent Red (red color) is used with the AP-Polymer anti-Rabbit IgG. DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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