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AB210061

DoubleStain IHC Kit: M&R on human tissue (HRP/Green & AP/Red)

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(1 Review)

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(6 Publications)

DoubleStain IHC Kit: M&R on human tissue (HRP/Green & AP/Red)( ab210061) is designed to be used with supplied mouse and rabbit primary antibodies on human tissue or cell samples. Suitable with formalin-fixed, paraffin-embedded (FFPE) or frozen tissue.

- Contains a HRP Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG and two distinct chromogens: Emerald Chromogen (Green) and Permanent Red (Red)
- Designed to prevent non-specific binding of endogenous biotin
- Organic Mounting Medium provided
- Available in different sizes to fit your experimental needs

Key facts

Sample types

Tissue

Reacts with

Human

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DoubleStain IHC Kit: M&R on human tissue (HRP/Green & AP/Red) (ab210061) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.

Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, AP-Polymer anti-Rabbit IgG, and two distinct chromogens, Emerald (green) and Permanent Red (red). User may apply the two enzyme conjugates onto the specimen at the same time and mix them on the slide. Simplified steps offer user much faster and quicker protocol than a sequential procedure. A second advantage is it allows the researcher to visualize when two proteins are co-localized because of the color change when the chromogens overlap that can be semi-quantitative. For example, if the area of co-localization stains blue, the antigen indicated by Emerald is expressed at higher concentration in the cell and if the color is purple, the antigen indicated by Permanent-Red is expressed at higher concentrations DoubleStain IHC Kit: M&R on human tissue (HRP/Green &AP/Red) is a non-biotin system that avoids endogenous biotin non-specific binding.

The Organic Mounting Medium is not supplied with the 120 mL kit.

The Permanent Red working solution is not stable. It must be used (freshly) immediately after it is made.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Pathogens (Basel, Switzerland) 13: PubMed39599571

2024

The Skin Test Predicts Clinic-Immunologic and Therapeutic Outcomes in Cutaneous Leishmaniasis.

Applications

Unspecified application

Species

Unspecified reactive species

Luiz H Guimarães,Evelyn Zacarias,Sandra T Nolasco,Almério N Filho,Jamile Lago,Paulo R L Machado,Joyce Oliveira,Lucas P Carvalho,Augusto Carvalho,Edgar M Carvalho,Sérgio Arruda

Indian journal of surgical oncology 14:186-193 PubMed36891422

2023

Detection, Role and Prognostic Value of Cancer-associated Fibroblasts in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Alexandrovna Oleynikova,I A Mikhailov,O A Kharlova,T Yu Zavidnyi,N V Danilova,P G Mal Kov

Parasite immunology 45:e12971 PubMed36695719

2023

Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen M Sandoval Pacheco,Gabriela V Araujo Flores,Aurea F Ferreira,Vânia L R da Matta,Claudia M de Castro Gomes,Wilfredo H Sosa-Ochoa,Concepción Zúniga,Fernando T Silveira,Carlos E P Corbett,Márcia D Laurenti

Journal of immunology research 2021:5596876 PubMed33937417

2021

Macrophage Polarization in the Skin Lesion Caused by Neotropical Species of sp.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen M Sandoval Pacheco,Gabriela V Araujo Flores,Kadir Gonzalez,Claudia M de Castro Gomes,Luiz F D Passero,Thaise Y Tomokane,Wilfredo Sosa-Ochoa,Concepción Zúniga,Jose Calzada,Azael Saldaña,Carlos E P Corbett,Fernando T Silveira,Marcia D Laurenti

British journal of haematology 184:578-593 PubMed30408155

2018

Mesenchymal stem cells gene signature in high-risk myeloma bone marrow linked to suppression of distinct IGFBP2-expressing small adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Syed J Mehdi,Sarah K Johnson,Joshua Epstein,Maurizio Zangari,Pingping Qu,Antje Hoering,Frits van Rhee,Carolina Schinke,Sharmilan Thanendrarajan,Bart Barlogie,Faith E Davies,Gareth J Morgan,Shmuel Yaccoby

Gastroenterology 155:892-908.e6 PubMed29864419

2018

Cigarette Smoke Induces Stem Cell Features of Pancreatic Cancer Cells via PAF1.

Applications

Unspecified application

Species

Unspecified reactive species

Rama Krishna Nimmakayala,Parthasarathy Seshacharyulu,Imayavaramban Lakshmanan,Satyanarayana Rachagani,Seema Chugh,Saswati Karmakar,Sanchita Rauth,Raghupathy Vengoji,Pranita Atri,Geoffrey A Talmon,Subodh M Lele,Lynette M Smith,Ishwor Thapa,Dhundy Bastola,Michel M Ouellette,Surinder K Batra,Moorthy P Ponnusamy
View all publications
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