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Abcam's Human on human IHC kit (HRP/DAB) (ab214749) is designed for staining human primary antibodies on human tissues without background staining.

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Reactive species

Human

Reactivity data

Application
IHC
Reactivity
Reacts
Dilution info
-
Notes

-

What's included?

6 mL
Components
20X DAB Chromogen
1 x 1.5 mL
5X Quenching Buffer
1 x 1.5 mL
Blocking Buffer A
1 x 6 mL
Blocking Buffer B
1 x 6 mL
DAB Substrate Buffer
1 x 12 mL
Human HRP Polymer
1 x 6 mL
Human Primer
1 x 6 mL

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Abcam's Human on human IHC kit (HRP/DAB) (ab214749) is designed for staining human primary antibodies on human tissues without background staining.

Key facts

Reactive species

Human

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Abcam's Human on human IHC kit (HRP/DAB) (ab214749) is designed for staining human primary antibodies on human tissues without background staining.

Antigen detection using a primary antibody of the same species as the test tissue yields high background when an indirect detection method is used. This severely limits the ability to screen human antibodies on human tissues. Abcam's Human on human IHC kit (HRP/DAB) (ab214749) is a ready to use system that results in excellent sensitivity and high specificity using a simple protocol. Furthermore, as the protocol uses a polymer-based, biotin-free system, it does not require blocking of endogenous biotin.  

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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