This IHC detection kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC).
Application | Reactivity | Dilution info | Notes |
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This IHC detection kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC).
This IHC detection kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and AEC chromogen. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.
Other IHC detection products
For alternative kits, see Anti-Rabbit HRP-AEC Micro-polymer IHC Kit Rabbit specific HRP/AEC IHC Detection Kit - Micro-polymer ab236468, and HRP-DAB micro-polymer IHC kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Terms & Conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Immunohistochemical staining of p53 in human colon adenocarcinoma with anti-p53 antibody at a dilution of 1/400 and detected using ab93686 (this kit is identical to ab93686, except that it uses the same HRP micro-polymer antibody used by Abcam's R&D team for antibody validation in IHC.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Immunohistochemical staining of smooth muscle actin in human small intestine with Anti-alpha smooth muscle Actin antibody [1A4] ab7817 at a dilution of 1/200 and detected using ab93686 (this kit is identical to ab93686, except that it uses the HRP micro-polymer secondary antibody used by Abcam's R&D team for antibody validation in IHC).
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