Skip to main content

This IHC detection kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC).

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer (AB236467), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer (AB236467), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Assay type
Micro-Polymer

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

What's included?

15 mL
Components
AEC Single Solution
1 x 15 mL
Goat anti-rabbit HRP Conjugate
1 x 15 mL
Hydrogen Peroxide Block
1 x 15 mL
Mouse Specifying Reagent (Complement)
1 x 15 mL
Protein block
1 x 15 mL

Recommended products

This IHC detection kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC).

Key facts

Detection method
Colorimetric
Assay type
Micro-Polymer

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

This IHC detection kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.

HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.

The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and AEC chromogen. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.

Other IHC detection products

For alternative kits, see Anti-Rabbit HRP-AEC Micro-polymer IHC Kit Rabbit specific HRP/AEC IHC Detection Kit - Micro-polymer ab236468, and HRP-DAB micro-polymer IHC kits.

For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer (ab236467), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer (ab236467)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Immunohistochemical staining of p53 in human colon adenocarcinoma with anti-p53 antibody at a dilution of 1/400 and detected using ab93686 (this kit is identical to ab93686, except that it uses the same HRP micro-polymer antibody used by Abcam's R&D team for antibody validation in IHC.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer (ab236467), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer (ab236467)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Immunohistochemical staining of smooth muscle actin in human small intestine with Anti-alpha smooth muscle Actin antibody [1A4] ab7817 at a dilution of 1/200 and detected using ab93686 (this kit is identical to ab93686, except that it uses the HRP micro-polymer secondary antibody used by Abcam's R&D team for antibody validation in IHC).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com