AB64264

Mouse and Rabbit Specific HRP/DAB Detection IHC kit

Name: HRP/DAB detection IHC kit, mouse & rabbit specific - 15 mL (ab64264) | Abcam
Brand: Abcam Limited
SKU: AB64264
Rating: 5 (3 reviews)

(3 Reviews)

(277 Publications)

HRP/DAB detection IHC kit, mouse & rabbit specific (ab64264) is an easy-to-use complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.

- Proven performance: cited in over 220 publications

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB Detection IHC kit (AB64264)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB Detection IHC kit (AB64264)

Staining of human estrogen receptor in breast carcinoma tissue using ab16660 - Rabbit monoclonal [SP1] to Estrogen Receptor, and ab64246 - Mouse & Rabbit HRP/DAB Detection Kit.

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB Detection IHC kit (AB64264)

Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1 : 450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).

Yu S.H., PLoS One 9 (12), Fig 7c. doi: 10.1371/journal.pone.0114649 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Key facts

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse and Rabbit specific HRP/DAB Detection IHC Kit ab64264 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.

It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.

In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.

For alternative streptavidin-biotin IHC detection kits see:
- Anti-Rabbit HRP-DAB IHC kit ab64261
- Anti-Mouse HRP-DAB IHC Kit ab64259

For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469

Alternatively, see HRP-AEC IHC detection kits.

For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

style

left-column

What's included?

{ "values": { "15mL": { "sellingSize": "15 mL", "publicAssetCode":"ab64264-15mL", "assetComponentDetails": [ { "size":"1 x 0.5 mL", "name":"50x DAB Chromogen", "number":"AB64264-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Streptavidin Peroxidase", "number":"AB64264-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"Biotinylated Goat Anti-Polyvalent", "number":"AB64264-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"DAB substrate", "number":"AB64264-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"Hydrogen Peroxide Block", "number":"AB64264-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"Protein block", "number":"AB64264-CMP05", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

style

left-column

Publications (277)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40459937

2025

Mutant THAP11 causes cerebellar neurodegeneration and triggers TREM2-mediated microglial activation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Eshu Ruan,Jingpan Lin,Zhao Chen,Qianai Sheng,Laiqiang Chen,Jiating He,Xuezhi Duan,Yiyang Qin,Tingting Xing,Sitong Yang,Mingtian Pan,Xiangyu Guo,Peng Yin,Xiao-Jiang Li,Hong Jiang,Shihua Li,Su Yang

Molecular medicine (Cambridge, Mass.) 31:207 PubMed40419958

2025

Impact of intermittent fasting versus vitamin D on high fat fructose-induced pancreatic steatosis: possible role of aquaporins.

Applications

Unspecified application

Species

Unspecified reactive species

Basma Adel Khattab,Maha Osman Hammad,Zienab Helmy Eldken,Doaa Hellal,Sherin Zohdy Mohamed,Noha Hammad Sakr

Cancers 17: PubMed40361322

2025

Analysis of Genotype and Expression of FTO and ALKBH5 in a MENA-Region Renal Cell Carcinoma Cohort.

Applications

Unspecified application

Species

Unspecified reactive species

Muna Abdalla Alhammadi,Burcu Yener Ilce,Poorna Manasa Bhamidimarri,Amal Bouzid,Nival Ali,Reem Sami Alhamidi,Alaa Mohamed Hamad,Mona Mahfood,Abdelaziz Tlili,Iman M Talaat,Rifat Hamoudi

Anatomia, histologia, embryologia 54:e70038 PubMed40251900

2025

Exploration of Dentition Development and Replacement in Two Forms of Mexican Tetra (Astyanax mexicanus).

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Fang,Devi Atukorallaya

Signal transduction and targeted therapy 10:109 PubMed40199859

2025

The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chandana Thimme Gowda,Mallikarjuna Siraganahalli Eshwaraiah,Jiaohong Wang,Youngyi Lim,Maria Lauda Tomasi,Nirmala Mavila,Komal Ramani

The Journal of clinical investigation 135: PubMed40091830

2025

Activating antiviral immune responses potentiates immune checkpoint inhibition in glioblastoma models.

Applications

Unspecified application

Species

Unspecified reactive species

Deepa Seetharam,Jay Chandar,Christian K Ramsoomair,Jelisah F Desgraves,Alexandra Alvarado Medina,Anna Jane Hudson,Ava Amidei,Jesus R Castro,Vaidya Govindarajan,Sarah Wang,Yong Zhang,Adam M Sonabend,Mynor J Mendez Valdez,Dragan Maric,Vasundara Govindarajan,Sarah R Rivas,Victor M Lu,Ritika Tiwari,Nima Sharifi,Emmanuel Thomas,Marcus Alexander,Catherine DeMarino,Kory Johnson,Macarena I De La Fuente,Ruham Alshiekh Nasany,Teresa Maria Rosaria Noviello,Michael E Ivan,Ricardo J Komotar,Antonio Iavarone,Avindra Nath,John Heiss,Michele Ceccarelli,Katherine B Chiappinelli,Maria E Figueroa,Defne Bayik,Ashish H Shah

Frontiers in pharmacology 16:1519066 PubMed40046735

2025

The clinically applied PARP inhibitor talazoparib ameliorates imiquimod-induced psoriasis in mice without reducing skin inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Petra Molnár,Máté Ágoston Demény,Beáta Várkonyi,Zsuzsanna Polgár,Ágnes Pór,Ilona Kovács,Andrea Szegedi,Attila Gábor Szöllősi,Magdolna Szántó

Journal of translational medicine 23:189 PubMed39956898

2025

MicroRNA-27a transfected dental pulp stem cells undergo odonto/osteogenic differentiation via targeting DKK3 and SOSTDC1 in Wnt/BMP signaling in vitro and enhance bone formation in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ziniu Yu,Nobuyuki Kawashima,Keisuke Sunada-Nara,Shihan Wang,Peifeng Han,Thoai Quoc Kieu,Chunmei Ren,Sonoko Noda,Kento Tazawa,Takashi Okiji

Nature communications 16:1650 PubMed39952912

2025

Expression of foetal gene Pontin is essential in protecting heart against pathological remodelling and cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Bayu Lestari,Ardiansah Bayu Nugroho,Thuy Anh Bui,Binh Nguyen,Nicholas Stafford,Sukhpal Prehar,Min Zi,Ryan Potter,Efta Triastuti,Florence M Baudoin,Alicia D'Souza,Xin Wang,Elizabeth J Cartwright,Delvac Oceandy

Biotechnologia 105:367-376 PubMed39844874

2025

Gliclazide protects ionizing radiation-induced intestinal injury in mice by inhibiting oxidative stress and caspase-3.

Applications

Unspecified application

Species

Unspecified reactive species

Soroush Arzani,Soghra Farzipour,Fereshteh Talebpour Amiri,Seyed Jalal Hosseinimehr

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse and Rabbit Specific HRP/DAB Detection IHC kit

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB Detection IHC kit (AB64264)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB Detection IHC kit (AB64264)

Key facts

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (277)

Complementary Products

Secondary Antibodies

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Product promise