Mouse and Rabbit specific HRP/DAB Detection IHC Kit ab64264 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.
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Publications
- International journal of biological sciences 20:880-8962024Nε-(1-Carboxymethyl)-L-lysine/RAGE Signaling Drives Metastasis and Cancer Stemness through ERK/NFκB axis in Osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing-Yu Chang,Kuo-Cheng Lan,Chia-Hung Wu,Meei-Ling Sheu,Rong-Sen Yang,Shing-Hwa LiuPubMed 38250151
- Reproduction (Cambridge, England) 167:2024Decreased NSD2 impairs stromal cell proliferation in human endometrium via reprogramming H3K36me2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan-Mei Qin,Xiao-Wei Wei,Jia-Yi Wu,Xue-Qing Liu,Yi LinPubMed 38236723
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
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Mouse and Rabbit specific HRP/DAB Detection IHC Kit ab64264 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.
Storage
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Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse and Rabbit specific HRP/DAB Detection IHC Kit ab64264 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.
For alternative streptavidin-biotin IHC detection kits see: - Anti-Rabbit HRP-DAB IHC kit Rabbit specific HRP/DAB Detection IHC Kit ab64261 - Anti-Mouse HRP-DAB IHC Kit Mouse specific HRP/DAB Detection IHC Kit ab64259 For high sensitivity HRP micro-polymer IHC detection kits see: - Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer ab236466 - Anti-Rabbit HRP-DAB Micro-polymer IHC Kit Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer ab236469 Alternatively, see . For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055. Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the .
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB Detection IHC kit (ab64264)
Staining of human estrogen receptor in breast carcinoma tissue using Anti-Estrogen Receptor alpha antibody [SP1] ab16660 - Rabbit monoclonal [SP1] to Estrogen Receptor, and ab64246 - Mouse & Rabbit HRP/DAB Detection Kit.
Yu S.H., PLoS One 9 (12), Fig 7c. doi: 10.1371/journal.pone.0114649 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB Detection IHC kit (ab64264)
Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).
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