Skip to main content

This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC).

Detection method

Colorimetric

Assay type

Micro-Polymer

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

15 mL
Components
50x DAB Chromogen
1 x 0.5 mL
DAB substrate
1 x 15 mL
Goat anti-rabbit HRP Conjugate
1 x 15 mL
Hydrogen Peroxide Block
1 x 15 mL
Mouse Specifying Reagent (Complement)
1 x 15 mL
Protein block
1 x 15 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC).

Key facts

Detection method

Colorimetric

Assay type

Micro-Polymer

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.

HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.

The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.

The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold). **Other IHC detection products** For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469, and . For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055. Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com