This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC).
Colorimetric
Micro-Polymer
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC).
Colorimetric
Micro-Polymer
Blue Ice
+4°C
+4°C
+4°C
This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.
The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold). **Other IHC detection products** For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer ab236469, and . For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055. Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the .
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skin. (A) The upper panel shows representative MMP-2 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX, and the lower panel shows bar graphs representing the expression of MMP-2 in the skin of CTRL and MFS mice at 12 weeks of age with or without DOX. (B) The upper panel shows representative MMP-9 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX treatment. The lower panel shows bar graphs representing the expression of MMP-9 in the skin of CTRL and MFS mice at 12 weeks of age treated with or without DOX.
Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the aorta.Immunohistochemistry staining images and bar graphs representing the expression of (A) MMP-2 and (B) MMP-9 in the aortic roots of CTRL and MFS mice at 12 weeks of age treated with or without DOX.
ab236466 Mouse and Rabbit Specific HRP-DAB Detection IHC Kit on Human Tonsil staining CD20
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Anti-TMEM119 antibody [28-3] - Microglial marker ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with Anti-TMEM119 antibody [28-3] - Microglial marker ab209064 at 1/2000 dilution followed by the same HRP micro-polymer goat anti-rabbit secondary antibody used in this kit. Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ab1220 staining human kidney sections by IHC-P using EXPOSE IHC detection kit ab80436 (this kit is identical to ab80436, except that it uses the same HRP polymer secondary antibody used by Abcam's R&D team for antibody validation). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH 6) for 30 minutes. The section was incubated with ab1220, 5 μg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com