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AB93697

Mouse and Rabbit Specific HRP Plus Detection IHC Kit (Ready To Use)

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(28 Publications)

The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP Plus Detection IHC Kit (Ready To Use) (AB93697)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP Plus Detection IHC Kit (Ready To Use) (AB93697)

Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.

Image from McGee-Lawrence ME et al., Biochem Biophys Res Commun. 2013;411(4):886-90. Fig 3.; doi: 10.1016/j.bbrc.2013.10.155 with permission from Elsevier.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

ab93697 does not contain chromogen or substrate.

Reacts with Mouse and Rabbit IgG (H+L).

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 15: PubMed40075982

2025

Immunohistochemical Characterization of Feline Giant Cell Tumor of Bone (GCTb): What We Know and What We Can Learn from the Human Counterpart.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Porcellato,Giuseppe Giglia,Leonardo Leonardi

Bratislavske lekarske listy 124:842-847 PubMed37874807

2023

The effect of protocatechuic acid on ovarian histopathology and reserve in rat ovarian torsion model.

Applications

Unspecified application

Species

Unspecified reactive species

Sevilay Erimsah,Ayhan Cetinkaya

International journal of molecular sciences 24: PubMed36835176

2023

Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Bosco,Lorenza Guarnieri,Saverio Nucera,Miriam Scicchitano,Stefano Ruga,Antonio Cardamone,Samantha Maurotti,Cristina Russo,Anna Rita Coppoletta,Roberta Macrì,Irene Bava,Federica Scarano,Fabio Castagna,Maria Serra,Rosamaria Caminiti,Jessica Maiuolo,Francesca Oppedisano,Sara Ilari,Filomena Lauro,Luigi Giancotti,Carolina Muscoli,Cristina Carresi,Ernesto Palma,Micaela Gliozzi,Vincenzo Musolino,Vincenzo Mollace

International journal of molecular sciences 24: PubMed36768133

2023

Irisin Modulates Inflammatory, Angiogenic, and Osteogenic Factors during Fracture Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Oranger,Roberta Zerlotin,Cinzia Buccoliero,Lorenzo Sanesi,Giuseppina Storlino,Ernestina Schipani,Kenneth Michael Kozloff,Giorgio Mori,Graziana Colaianni,Silvia Colucci,Maria Grano

Viruses 14: PubMed36146800

2022

West Nile Virus and Usutu Virus: A Post-Mortem Monitoring Study in Wild Birds from Rescue Centers, Central Italy.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Giglia,Giulia Mencattelli,Elvio Lepri,Gianfilippo Agliani,Marco Gobbi,Andrea Gröne,Judith M A van den Brand,Giovanni Savini,Maria Teresa Mandara

AMB Express 12:113 PubMed36057049

2022

Relationship between mucosal TNF-α expression and Th1, Th17, Th22 and Treg responses in Helicobacter pylori infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ghorbanali Rahimian,Milad Shahini Shams Abadi,Yousef Mirzaei,Ali Hussein Mer,Reza Ahmadi,Fatemeh Azadegan-Dehkordi

Molecular and cellular endocrinology 542:111519 PubMed34843900

2021

GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis.

Applications

Unspecified application

Species

Unspecified reactive species

Matheus Felipe Fonseca Gonçalves,Samyra Maria Dos Santos Nassif Lacerda,Nathália de Lima E Martins Lara,Carolina Felipe Alves de Oliveira,André Felipe Almeida Figueiredo,Marcos Rocha Gouvêa Brener,Marina Alcântara Cavalcante,Anderson Kenedy Santos,Gabriel Henrique Campolina-Silva,Vivian Vasconcelos Costa,Ana Clara Campideli Santana,Roberta Araújo Lopes,Raphael Escorsim Szawka,Guilherme Mattos Jardim Costa

International journal of experimental pathology 102:249-259 PubMed34762773

2021

Correlation between aryl hydrocarbon receptor and IL-17 and Foxp3 T-cell infiltration in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Soheila Fattahi,Monireh Karimi,Mahdi Ghatreh-Samani,Fatemeh Taheri,Hedayatollah Shirzad,Faramarz Mohammad Alibeigi,Maryam Anjomshoa,Nader Bagheri

Indian journal of ophthalmology 69:1219-1223 PubMed33913864

2021

Investigating the effects of carvacrol in rats using oxygen-induced retinopathy model.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulgani Kaymaz,Fatih Ulaş,Ayhan Çetinkaya,Sevilay Erimşah

Cancer management and research 13:3113-3122 PubMed33859497

2021

Clinical Value of Detecting Tumor Endothelial Marker 8 (ANTXR1) as a Biomarker in the Diagnosis and Prognosis of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Łukasz Pietrzyk,Agnieszka Korolczuk,Małgorzata Matysek,Marcin B Arciszewski,Kamil Torres
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