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Mouse on mouse polymer IHC kit ab269452 allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.

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Sample type

Tissue

Reactive species

Mouse

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

15 mL
Components
HRP polymer detector (ready to use)
1 x 15 mL
Mouse on mouse blocking reagent (ready to use)
1 x 15 mL

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Mouse on mouse polymer IHC kit ab269452 allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.

Key facts

Sample type

Tissue

Reactive species

Mouse

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse on mouse polymer IHC kit ab269452 allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.

The mouse on mouse kit includes an Fab'anti-mouse IgG(H+L) blocking reagent and an HRP polymer conjugated Fab'anti-mouse IgG(H+L) detection reagent.

This kit replaces mouse on mouse kit ab127055. The reagents used in this kit have been carefully optimized to match the performance of ab127055 (see validation images). This kit does not include hydrogen peroxide blocking reagent: we recommend hydrogen peroxide blocking reagent ab64218.

In the mouse on mouse IHC protocol:

- Cover the tissue section with the blocking reagent (before the addition of the mouse primary antibody) for 30-60 minutes. Wash 2-3 times.
The blocking reagent binds to endogenous mouse antibody in the tissue and prevents the binding of anti-mouse secondary antibody to those sites.

- Incubate with primary antibody of your choice raised in mouse. Wash 2-3 times.

- Incubate with HRP polymer detector reagent for ~15 minutes. Wash 2-3 times.

- Stain with a chromogen (usually DAB, eg. ab64238) for detection.

Otherwise, follow a conventional IHC protocol for DAB staining.

The detector and blocker have <1% reactivity to human and rabbit antibodies.

**Other products for IHC**Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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