Mouse on mouse IHC kit allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.
- Cited in over 10 publications
- HRP polymer for strong, specific detection
- Minimal cross-reactivity with human/rabbit
- Compatible with standard DAB staining
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Publications
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- Biology of sex differences 14:892023Sex and interspecies differences in ESR2-expressing cell distributions in mouse and rat brains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasahiro Morishita,Shimpei Higo,Kinuyo Iwata,Hirotaka IshiiPubMed 38111056
- PloS one 18:e02910832023Preclinical safety evaluation of continuous UV-A lighting in an operative setting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRachael Guenter,Rui Zheng-Pywell,Brendon Herring,Madisen Murphy,Jeremy Foote,Kevin Benner,J Bart RosePubMed 37976243
- Cell death discovery 9:3602023PTP4A1 promotes oral squamous cell carcinoma (OSCC) metastasis through altered mitochondrial metabolic reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Liu,Wen Si,Bo Wei,Xuan Zhang,Peng ChenPubMed 37773151
- Cell death & disease 14:6122023KLF4 regulates skeletal muscle development and regeneration by directly targeting P57 and Myomixer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShufang Cai,Xiaoyu Wang,Rong Xu,Ziyun Liang,Qi Zhu,Meilin Chen,Zhuhu Lin,Chenggan Li,Tianqi Duo,Xian Tong,Enru Li,Zuyong He,Xiaohong Liu,Yaosheng Chen,Delin MoPubMed 37723138
- Cancers 15:2023α-Mangostin Promotes In Vitro and In Vivo Degradation of Androgen Receptor and AR-V7 Splice Variant in Prostate Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMirielle C Nauman,Jong Hoon Won,Sakina M Petiwala,Bhaskar Vemu,Hyun Lee,Maria Sverdlov,Jeremy J JohnsonPubMed 37046780
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e226122022Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoseanne Rosario,Hazel L Stewart,Nila Roy Choudhury,Gracjan Michlewski,Nicholas Charlet-Berguerand,Richard A AndersonPubMed 36250920
- European cells & materials 43:228-2512022Histology of tendon and enthesis - suitable techniques for specific research questions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesN Angrisani,E Willbold,A Kampmann,A Derksen,J ReifenrathPubMed 35608562
- Experimental dermatology 31:1355-13632022Autologously transplanted dermis-derived cells alleviated monobenzone-induced vitiligo in mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAslı Erdoğan,Hasan Serdar Mutlu,Seyhun SolakoğluPubMed 35538739
- PloS one 17:e02642662022Protocol for chronic hepatitis B virus infection mouse model development by patient-derived orthotopic xenografts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksey M Nagornykh,Marina A Tyumentseva,Aleksandr I Tyumentsev,Vasiliy G AkimkinPubMed 35196351
- Oxidative medicine and cellular longevity 2022:179289420225-Lipoxygenase Inhibition Protects Retinal Pigment Epithelium from Sodium Iodate-Induced Ferroptosis and Prevents Retinal Degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJong-Jer Lee,Guo-Ping Chang-Chien,Sufan Lin,Yu-Ting Hsiao,Mu-Chan Ke,Alexander Chen,Tsu-Kung LinPubMed 35251467
Key facts
- Sample type
- Tissue
- Reactive species
- Mouse
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
Mouse on mouse IHC kit allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.
- Cited in over 10 publications
- HRP polymer for strong, specific detection
- Minimal cross-reactivity with human/rabbit
- Compatible with standard DAB staining
Key facts
- Sample type
- Tissue
- Reactive species
- Mouse
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse on mouse polymer IHC kit ab269452 allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.
The mouse on mouse kit includes an Fab'anti-mouse IgG(H+L) blocking reagent and an HRP polymer conjugated Fab'anti-mouse IgG(H+L) detection reagent.
This kit replaces mouse on mouse kit ab127055. The reagents used in this kit have been carefully optimized to match the performance of ab127055 (see validation images). This kit does not include hydrogen peroxide blocking reagent: we recommend hydrogen peroxide blocking reagent Hydrogen Peroxide Blocking Reagent ab64218.
In the mouse on mouse IHC protocol:
- Cover the tissue section with the blocking reagent (before the addition of the mouse primary antibody) for 30-60 minutes. Wash 2-3 times.
The blocking reagent binds to endogenous mouse antibody in the tissue and prevents the binding of anti-mouse secondary antibody to those sites.
- Incubate with primary antibody of your choice raised in mouse. Wash 2-3 times.
- Incubate with HRP polymer detector reagent for ~15 minutes. Wash 2-3 times.
- Stain with a chromogen (usually DAB, eg. DAB Substrate Kit ab64238) for detection.
Otherwise, follow a conventional IHC protocol for DAB staining.
The detector and blocker have <1% reactivity to human and rabbit antibodies.
Other products for IHC
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (ab269452)
Mouse on Mouse IHC test with comparison to ab127055.
Mouse on mouse polymer IHC kit ab269452 (panel B) was tested using an anti-actin mouse monoclonal on formalin-fixed paraffin embedded mouse tissue sections.
Equivalent staining was seen using the same antibody and serial tissue section with ab127055 (panel C).
A control without primary antibody showed no staining (panel A).
Antigen retrieval was using 1mM EDTA, pH 9.0 for 15 minutes. The detection reagents in the mouse on mouse kits were incubated with the section for 15 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (ab269452)
Mouse on Mouse IHC test with comparison to ab127055.
Mouse on mouse polymer IHC kit ab269452 (panel B) was tested using an anti-actin mouse monoclonal on formalin-fixed paraffin embedded mouse tissue sections.
Equivalent staining was seen using the same antibody and serial tissue section with ab127055 (panel C).
A control without primary antibody showed no staining (panel A).
Antigen retrieval was using 1mM EDTA, pH 9.0 for 15 minutes. The detection reagents in the mouse on mouse kits were incubated with the section for 15 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (ab269452)
Mouse on Mouse IHC test with comparison to ab127055.
Mouse on mouse polymer IHC kit ab269452 (panel B) was tested using an anti-actin mouse monoclonal on formalin-fixed paraffin embedded mouse tissue sections.
Equivalent staining was seen using the same antibody and serial tissue section with ab127055 (panel C).
A control without primary antibody showed no staining (panel A).
Antigen retrieval was using 1mM EDTA, pH 9.0 for 15 minutes. The detection reagents in the mouse on mouse kits were incubated with the section for 15 minutes.
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