AB269452

Mouse on Mouse Polymer IHC Kit

Name: Mouse on mouse IHC kit (polymer) - 15, 60 mL (ab269452) | Abcam
Brand: Abcam Limited
SKU: AB269452
Price: 650 USD
Availability: InStock

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(20 Publications)

Mouse on Mouse Polymer IHC Kit (ab269452) contains ready-to-use HRP polymer detector and Mouse on Mouse (MOM) blocking reagent. Allows mouse antibodies to be used on mouse tissues in IHC applications.

- Prevents high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues
- Available in different sizes to fit your experimental needs
- Proven performance: cited in over 20 publications

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (AB269452)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (AB269452)

Mouse on Mouse IHC test with comparison to ab127055.

Mouse on mouse polymer IHC kit ab269452 (panel B) was tested using an anti-actin mouse monoclonal on formalin-fixed paraffin embedded mouse tissue sections.

Equivalent staining was seen using the same antibody and serial tissue section with ab127055 (panel C).

A control without primary antibody showed no staining (panel A).

Antigen retrieval was using 1mM EDTA, pH 9.0 for 15 minutes. The detection reagents in the mouse on mouse kits were incubated with the section for 15 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (AB269452)

Mouse on Mouse IHC test with comparison to ab127055.

Mouse on mouse polymer IHC kit ab269452 (panel B) was tested using an anti-actin mouse monoclonal on formalin-fixed paraffin embedded mouse tissue sections.

Equivalent staining was seen using the same antibody and serial tissue section with ab127055 (panel C).

A control without primary antibody showed no staining (panel A).

Antigen retrieval was using 1mM EDTA, pH 9.0 for 15 minutes. The detection reagents in the mouse on mouse kits were incubated with the section for 15 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (AB269452)

Mouse on Mouse IHC test with comparison to ab127055.

Mouse on mouse polymer IHC kit ab269452 (panel B) was tested using an anti-actin mouse monoclonal on formalin-fixed paraffin embedded mouse tissue sections.

Equivalent staining was seen using the same antibody and serial tissue section with ab127055 (panel C).

A control without primary antibody showed no staining (panel A).

Antigen retrieval was using 1mM EDTA, pH 9.0 for 15 minutes. The detection reagents in the mouse on mouse kits were incubated with the section for 15 minutes.

Key facts

Sample types

Tissue

Reacts with

Mouse

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse on mouse polymer IHC kit ab269452 allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.

The mouse on mouse kit includes an Fab'anti-mouse IgG(H+L) blocking reagent and an HRP polymer conjugated Fab'anti-mouse IgG(H+L) detection reagent.

This kit replaces mouse on mouse kit ab127055. The reagents used in this kit have been carefully optimized to match the performance of ab127055 (see validation images). This kit does not include hydrogen peroxide blocking reagent: we recommend hydrogen peroxide blocking reagent ab64218.

In the mouse on mouse IHC protocol:

- Cover the tissue section with the blocking reagent (before the addition of the mouse primary antibody) for 30-60 minutes. Wash 2-3 times.
The blocking reagent binds to endogenous mouse antibody in the tissue and prevents the binding of anti-mouse secondary antibody to those sites.

- Incubate with primary antibody of your choice raised in mouse. Wash 2-3 times.

- Incubate with HRP polymer detector reagent for ~15 minutes. Wash 2-3 times.

- Stain with a chromogen (usually DAB, eg. ab64238) for detection.

Otherwise, follow a conventional IHC protocol for DAB staining.

The detector and blocker have <1% reactivity to human and rabbit antibodies.

Other products for IHC

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112267 PubMed40248123

2025

Parp3 assists muscle function and skeletal muscle differentiation by selectively adjusting H3K27me3 enrichment.

Applications

Unspecified application

Species

Unspecified reactive species

Zuleyha Yildirim,Aurélia Noll,Kathline Martin-Hernandez,Jean-Christophe Amé,Najat Hanini,Nadia Messaddeq,Isabelle Robert,Bernardo Reina San Martin,Gunn Hildrestrand,Magnar Bjoras,Françoise Dantzer

Nature communications 15:9334 PubMed39472558

2024

Protein O-GlcNAcylation coupled to Hippo signaling drives vascular dysfunction in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Lei,Qiangyun Liu,Binggui Chen,Fangfang Wu,Yiming Li,Xue Dong,Nina Ma,Ziru Wu,Yanfang Zhu,Lu Wang,Yuxin Fu,Yuming Liu,Yinting Song,Mei Du,Heng Zhang,Jidong Zhu,Timothy J Lyons,Ting Wang,Junhao Hu,Heping Xu,Mei Chen,Hua Yan,Xiaohong Wang

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39406519

2024

The Excessive Tonic Inhibition of the Peri-infarct Cortex Depresses Low Gamma Rhythm Power During Poststroke Recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Alasoadura,Juliette Leclerc,Mahmoud Hazime,Jérôme Leprince,David Vaudry,Julien Chuquet

Science advances 10:eadh4435 PubMed38489371

2024

Increased genomic instability and reshaping of tissue microenvironment underlie oncogenic properties of mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro D'Ambrosio,Davide Bressan,Elisa Ferracci,Francesco Carbone,Patrizia Mulè,Federico Rossi,Caterina Barbieri,Elisa Sorrenti,Gaia Fiaccadori,Thomas Detone,Elena Vezzoli,Salvatore Bianchi,Chiara Sartori,Simona Corso,Akihisa Fukuda,Giovanni Bertalot,Andrea Falqui,Mattia Barbareschi,Alessandro Romanel,Diego Pasini,Fulvio Chiacchiera

Biology of sex differences 14:89 PubMed38111056

2023

Sex and interspecies differences in ESR2-expressing cell distributions in mouse and rat brains.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiro Morishita,Shimpei Higo,Kinuyo Iwata,Hirotaka Ishii

PloS one 18:e0291083 PubMed37976243

2023

Preclinical safety evaluation of continuous UV-A lighting in an operative setting.

Applications

Unspecified application

Species

Unspecified reactive species

Rachael Guenter,Rui Zheng-Pywell,Brendon Herring,Madisen Murphy,Jeremy Foote,Kevin Benner,J Bart Rose

Cell death discovery 9:360 PubMed37773151

2023

PTP4A1 promotes oral squamous cell carcinoma (OSCC) metastasis through altered mitochondrial metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liu,Wen Si,Bo Wei,Xuan Zhang,Peng Chen

Cell death & disease 14:612 PubMed37723138

2023

KLF4 regulates skeletal muscle development and regeneration by directly targeting P57 and Myomixer.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang Cai,Xiaoyu Wang,Rong Xu,Ziyun Liang,Qi Zhu,Meilin Chen,Zhuhu Lin,Chenggan Li,Tianqi Duo,Xian Tong,Enru Li,Zuyong He,Xiaohong Liu,Yaosheng Chen,Delin Mo

Cancers 15: PubMed37046780

2023

α-Mangostin Promotes In Vitro and In Vivo Degradation of Androgen Receptor and AR-V7 Splice Variant in Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mirielle C Nauman,Jong Hoon Won,Sakina M Petiwala,Bhaskar Vemu,Hyun Lee,Maria Sverdlov,Jeremy J Johnson

Cancer research 83:195-218 PubMed36409826

2022

Aberrant L-Fucose Accumulation and Increased Core Fucosylation Are Metabolic Liabilities in Mesenchymal Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Pieri,Alberto L Gallotti,Denise Drago,Manuela Cominelli,Ilaria Pagano,Valentina Conti,Silvia Valtorta,Angela Coliva,Sara Lago,Daniela Michelatti,Luca Massimino,Federica Ungaro,Laura Perani,Antonello Spinelli,Antonella Castellano,Andrea Falini,Alessio Zippo,Pietro L Poliani,Rosa Maria Moresco,Annapaola Andolfo,Rossella Galli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse on Mouse Polymer IHC Kit

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (AB269452)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (AB269452)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (AB269452)

Key facts

Sample types

Reacts with

Complementary Products

Secondary Antibodies

Reagents, Controls & Accessories

Sample Prep & Detection Kits

Primary Antibodies

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (20)

Product promise