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Mouse specific HRP/AEC IHC Detection Kit ab64258 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse Specific HRP/AEC Detection IHC kit (AB64258), expandable thumbnail

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

What's included?

15 mL
Components
AEC Single Solution
1 x 15 mL
Biotinylated goat anti-mouse IgG(H+L) (ab64255)
1 x 15 mL
Hydrogen Peroxide Block
1 x 15 mL
Protein block
1 x 15 mL
Streptavidin Peroxidase
1 x 15 mL

Recommended products

Mouse specific HRP/AEC IHC Detection Kit ab64258 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Mouse specific HRP/AEC IHC Detection Kit ab64258 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.

It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.

In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC.

For alternative AEC streptavidin-biotin IHC detection kits see:
- anti-Mouse / anti-Rabbit HRP-AEC IHC kit Mouse and Rabbit Specific HRP/AEC Detection IHC Kit ab93705
- Anti-Rabbit HRP-AEC IHC Kit Rabbit specific HRP/AEC Detection IHC Kit ab64260

For high sensitivity AEC HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer ab236467
- Anti-Rabbit HRP-AEC Micro-polymer IHC Kit Rabbit specific HRP/AEC IHC Detection Kit - Micro-polymer ab236468

Alternatively, see HRP-DAB IHC detection kits.

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse Specific HRP/AEC Detection IHC kit (ab64258), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse Specific HRP/AEC Detection IHC kit (ab64258)

    Immunohistochemical analysis of Bcl-2 expression in Human tonsil, using ab64258 Detection Kit.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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