Skip to main content

Mouse specific HRP/DAB IHC Detection Kit ab64259 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

15 mL
Components
50x DAB Chromogen
1 x 0.5 mL
Biotinylated goat anti-mouse IgG(H+L) (ab64255)
1 x 15 mL
DAB substrate
1 x 15 mL
Hydrogen Peroxide Block
1 x 15 mL
Protein block
1 x 15 mL
Streptavidin Peroxidase
1 x 15 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Mouse specific HRP/DAB IHC Detection Kit ab64259 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse specific HRP/DAB IHC Detection Kit ab64259 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.

It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.

In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.

For alternative streptavidin-biotin IHC detection kits see: - Anti-Mouse / anti-Rabbit HRP-DAB IHC kit ab64264 - Anti-Rabbit HRP-DAB IHC Kit ab64261 For high sensitivity HRP micro-polymer IHC detection kits see: - Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466 - Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469 Alternatively, see . For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055. Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com