AB64259

Mouse specific HRP/DAB Detection IHC Kit

Name: Mouse specific HRP/DAB Detection IHC Kit (ab64259) | Abcam
Brand: Abcam Limited
SKU: AB64259
Price: 335 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(56 Publications)

Mouse specific HRP/DAB IHC Detection Kit (ab64259) is a complete staining kit to detect a anti-mouse primary antibody using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody. Uses DAB Chromogen.

- Cited in over 45 publications.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse specific HRP/DAB Detection IHC Kit (AB64259)

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PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse specific HRP/DAB Detection IHC Kit (AB64259)

Immunohistochemical analysis staining SMA or CD68 in paraffin embedded mouse liver tissue sections Samples were incubated with anti-α- SMA (ab7817) or anti-CD68 (ab49777). A Mouse specific HRP/DAB Detection IHC Kit (ab64259 was used as a stain and Mayer's hematoxylin was used as a counterstain. Top panel of images staining CD68 and bottom panel staining α-SMA.

Jovicic N et al., PLoS One 10(7). Fig 6D & E. DOI: 10.1371/journal.pone.0134089. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

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Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse specific HRP/DAB Detection IHC Kit (AB64259)

Human colon carcinoma stained with anti p53 antibody using Anti Mouse HRP/DAB Detection Kit (ab64259).

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse specific HRP/DAB IHC Detection Kit (ab64259) is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.

It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.

How the kit works
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.

For alternative streptavidin-biotin IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-DAB IHC kit ab64264
- Anti-Rabbit HRP-DAB IHC Kit ab64261

For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469

Alternatively, see HRP-AEC IHC detection kits.

For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

For alternative streptavidin-biotin IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-DAB IHC kit ab64264
- Anti-Rabbit HRP-DAB IHC Kit ab64261

For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

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Publications (56)

Recent publications for all applications. Explore the full list and refine your search

Biomedical reports 23:119 PubMed40463397

2025

Relationship between the protein expression of ARID1A, ARID1B and ARID2 with the clinicopathological characteristics of colorectal cancer.

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Unspecified reactive species

Wariya Mongkolwat,Phattarapon Sonthi,Keerakarn Somsuan,Siripat Aluksanasuwan,Ratirath Samol,Natthiya Sakulsak,Sasithorn Wanna-Udom

Oncology research 33:641-651 PubMed40109863

2025

Astragalus polysaccharide enhances the therapeutic efficacy of cisplatin in triple-negative breast cancer through multiple mechanisms.

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Unspecified reactive species

L I Sun,Shichao Zhuo,Xiaoxin Li,Husheng Kong,Weiwei DU,Chong Zhou,Junxing Huang

Cells 13: PubMed39056783

2024

The EphA2 Receptor Regulates Invasiveness and Drug Sensitivity in Canine and Human Osteosarcoma Cells.

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Unspecified reactive species

Evelyn D Harris,Jessica C Sharpe,Timothy Strozen,Shabnam Abdi,Maya Kliewer,Malkon G Sanchez,Natacha S Hogan,Valerie MacDonald-Dickinson,Franco J Vizeacoumar,Behzad M Toosi

International journal of molecular sciences 25: PubMed38928267

2024

Arginine Vasotocin Directly Regulates Spermatogenesis in Adult Zebrafish () Testes.

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Maya Zanardini,Weimin Zhang,Hamid R Habibi

Biomolecules & therapeutics 32:432-441 PubMed38835111

2024

Extracellular Vesicles Derived from Adipose Stem Cells Alleviate Systemic Sclerosis by Inhibiting TGF-β Pathway.

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Unspecified reactive species

Eunae Kim,Hark Kyun Kim,Jae Hoon Sul,Jeongmi Lee,Seung Hyun Baek,Yoonsuk Cho,Jihoon Han,Junsik Kim,Sunyoung Park,Jae Hyung Park,Yong Woo Cho,Dong-Gyu Jo

Neurotrauma reports 5:95-116 PubMed38404523

2024

Blood-Brain Barrier Dysfunction Predicts Microglial Activation After Traumatic Brain Injury in Juvenile Rats.

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Tabitha R F Green,Tina Nguyen,Veronika Dunker,Danielle Ashton,J Bryce Ortiz,Sean M Murphy,Rachel K Rowe

NPJ biofilms and microbiomes 10:3 PubMed38233485

2024

Nisin lantibiotic prevents NAFLD liver steatosis and mitochondrial oxidative stress following periodontal disease by abrogating oral, gut and liver dysbiosis.

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Ryutaro Kuraji,Changchang Ye,Chuanjiang Zhao,Li Gao,April Martinez,Yukihiro Miyashita,Allan Radaic,Pachiyappan Kamarajan,Charles Le,Ling Zhan,Helene Range,Masataka Sunohara,Yukihiro Numabe,Yvonne L Kapila

iScience 26:108384 PubMed38047082

2023

Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs.

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Unspecified reactive species

Kumarjeet Banerjee,Shekhar Saha,Shaoli Das,Suman Ghosal,Indranil Ghosh,Abhimanyu Basu,Siddhartha S Jana

International journal of molecular sciences 24: PubMed37895104

2023

Human Non-Small Cell Lung Cancer-Chicken Embryo Chorioallantoic Membrane Tumor Models for Experimental Cancer Treatments.

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Unspecified reactive species

Jing Li,Tereza Brachtlova,Ida H van der Meulen-Muileman,Stijn Kleerebezem,Chang Liu,Peiyu Li,Victor W van Beusechem

American journal of physiology. Lung cellular and molecular physiology 325:L568-L579 PubMed37697923

2023

Sex-specific lung inflammation and mitochondrial damage in a model of electronic cigarette exposure in asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Ae Song,Ji Young Kim,Matthew W Gorr,Roy A Miller,Manjula Karpurapu,Jackie Nguyen,Devki Patel,Kellie J Archer,Navjot Pabla,Peter G Shields,Loren E Wold,John W Christman,Sangwoon Chung

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse specific HRP/DAB Detection IHC Kit

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse specific HRP/DAB Detection IHC Kit (AB64259)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse specific HRP/DAB Detection IHC Kit (AB64259)

Key facts

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (56)

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