Mouse specific HRP/DAB IHC Detection Kit ab64259 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.
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- NPJ biofilms and microbiomes 10:32024Nisin lantibiotic prevents NAFLD liver steatosis and mitochondrial oxidative stress following periodontal disease by abrogating oral, gut and liver dysbiosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyutaro Kuraji,Changchang Ye,Chuanjiang Zhao,Li Gao,April Martinez,Yukihiro Miyashita,Allan Radaic,Pachiyappan Kamarajan,Charles Le,Ling Zhan,Helene Range,Masataka Sunohara,Yukihiro Numabe,Yvonne L KapilaPubMed 38233485
- iScience 26:1083842023Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKumarjeet Banerjee,Shekhar Saha,Shaoli Das,Suman Ghosal,Indranil Ghosh,Abhimanyu Basu,Siddhartha S JanaPubMed 38047082
- International journal of molecular sciences 24:2023Human Non-Small Cell Lung Cancer-Chicken Embryo Chorioallantoic Membrane Tumor Models for Experimental Cancer Treatments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Li,Tereza Brachtlova,Ida H van der Meulen-Muileman,Stijn Kleerebezem,Chang Liu,Peiyu Li,Victor W van BeusechemPubMed 37895104
- eNeuro 10:2023Single-Nucleus Profiling Identifies Accelerated Oligodendrocyte Precursor Cell Senescence in a Mouse Model of Down Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBianca Rusu,Bharti Kukreja,Taiyi Wu,Sophie J Dan,Min Yi Feng,Brian T KalishPubMed 37491366
- Journal of biomechanics 148:1114722023Toward defining the role of the synovium in mitigating normal articular cartilage wear and tear.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew J Pellicore,Lianna R Gangi,Lance A Murphy,Andy J Lee,Timothy Jacobsen,Hagar M Kenawy,Roshan P Shah,Nadeen O Chahine,Gerard A Ateshian,Clark T HungPubMed 36753853
- Cell biology international 46:2232-22452022Apoptotic susceptibility of pancreatic alpha cells to environmentally relevant dose levels of bisphenol-A versus dibutyl phthalate is mediated by HSP60/caspase-3 expression in male albino rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed A Morsi,Ezat A Mersal,Ahmed O Alsabih,Ahmed M Abdelmoneim,Eman M Sakr,Shaimaa Alakabawy,Riham G Elfawal,Mohammed Naji,Hatem J Aljanfawe,Farah H Alshateb,Tamer M ShawkyPubMed 36168861
- Cell death & disease 13:6032022Lomitapide, a cholesterol-lowering drug, is an anticancer agent that induces autophagic cell death via inhibiting mTOR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoah Lee,Seung Ju Park,Seulgi Lee,Jinwook Lee,Eunbeol Lee,Eun-Seon Yoo,Won-Suk Chung,Jong-Woo Sohn,Byung-Chul Oh,Seyun KimPubMed 35831271
- Journal of lasers in medical sciences 13:e212022Effect of Simvastatin and Low-Level Laser Therapy on Sutural Bone Formation After Expansion in Rats: Biomechanical, Computed Tomography and Immunohistochemical Assessment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArash Farzan,Ali Moshiri,Sina Andalib,Mostafa Shamsi,Nima MotamedPubMed 35996495
- Frontiers in pharmacology 12:7146832021Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNemanja Jovicic,Ivica Petrovic,Nada Pejnovic,Biljana Ljujic,Marina Miletic Kovacevic,Sladjana Pavlovic,Ilija Jeftic,Aleksandar Djukic,Ivan Srejovic,Vladimir Jakovljevic,Miodrag L LukicPubMed 34803672
- Experimental biology and medicine (Maywood, N.J.) 246:1668-16792021Strategies for intra-amniotic administration of fetal therapy in a rabbit model of intrauterine growth restriction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMari Kinoshita,Fàtima Crispi,Carla Loreiro,Eduard Gratacós,Míriam Illa,Mònica ZamoraPubMed 33794699
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
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Mouse specific HRP/DAB IHC Detection Kit ab64259 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.
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- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse specific HRP/DAB IHC Detection Kit ab64259 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.
For alternative streptavidin-biotin IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-DAB IHC kit Mouse and Rabbit Specific HRP/DAB Detection IHC kit ab64264
- Anti-Rabbit HRP-DAB IHC Kit Rabbit specific HRP/DAB Detection IHC Kit ab64261
For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer ab236469
Alternatively, see HRP-AEC IHC detection kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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2 product images
Jovicic N et al., PLoS One 10(7). Fig 6D & E. DOI: 10.1371/journal.pone.0134089. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse specific HRP/DAB Detection IHC Kit (ab64259)
Immunohistochemical analysis staining SMA or CD68 in paraffin embedded mouse liver tissue sections Samples were incubated with anti-α- SMA (Anti-alpha smooth muscle Actin antibody [1A4] ab7817) or anti-CD68 (ab49777). A Mouse specific HRP/DAB Detection IHC Kit (ab64259 was used as a stain and Mayer's hematoxylin was used as a counterstain. Top panel of images staining CD68 and bottom panel staining α-SMA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse specific HRP/DAB Detection IHC Kit (ab64259)
Human colon carcinoma stained with anti p53 antibody using Anti Mouse HRP/DAB Detection Kit (ab64259).
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