Rabbit specific HRP/AEC Detection IHC Kit
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(3 Publications)
- Red final stain is created when the HRP is bound to site of the primary antibody by reacting with AEC
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific HRP/AEC Detection IHC Kit (AB64260)
Human tonsil stained with anti-CD3 antibody using Anti Rabbit HRP/AEC Detection Kit (ab64260)
Reactivity data
Product details
Rabbit specific HRP/AEC IHC Detection Kit ab64260 is a complete kit to detect a primary antibody raised in rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC.
For alternative AEC streptavidin-biotin IHC detection kits see:
- anti-Mouse / anti-Rabbit HRP-AEC IHC kit ab93705
- Anti-Mouse HRP-AEC IHC Kit ab64258
For high sensitivity AEC HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467
- Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468
Alternatively, see HRP-DAB IHC detection kits.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
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Publications (3)
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Proceedings of SPIE--the International Society for Optical Engineering 12039: PubMed37817877
2022
Applications
Unspecified application
Species
Unspecified reactive species
Cancer immunology, immunotherapy : CII 69:1461-1475 PubMed32285171
2020
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Unspecified application
Species
Unspecified reactive species
PloS one 12:e0181507 PubMed28746417
2017
Applications
IHC-P
Species
Rat
Product promise
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