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Rabbit specific HRP/DAB IHC Detection Kit ab64261 is a complete kit to detect a primary antibody raised in rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit secondary antibody.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Rabbit specific HRP/DAB IHC Detection Kit ab64261 is a complete kit to detect a primary antibody raised in rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit secondary antibody.
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Rabbit specific HRP/DAB IHC Detection Kit ab64261 is a complete kit to detect a primary antibody raised in rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.
For alternative streptavidin-biotin IHC detection kits see: - Anti-Mouse / anti-Rabbit HRP-DAB IHC kit ab64264 - Anti-Mouse HRP-DAB IHC Kit ab64259 For high sensitivity HRP micro-polymer IHC detection kits see: - Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466 - Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469 Alternatively, see . Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the .
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